Strain Fitness in Escherichia coli BW25113 around b1548

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttfaQ and stfQ overlap by 1 nucleotidesstfQ and nohA overlap by 8 nucleotidesnohA and ynfO are separated by 388 nucleotidesynfO and ydfO are separated by 57 nucleotides b1546: tfaQ - Qin prophage; predicted tail fibre assembly protein (NCBI), at 1,632,334 to 1,632,909 tfaQ b1547: stfQ - Qin prophage; predicted side tail fibre assembly protein (NCBI), at 1,632,909 to 1,633,871 stfQ b1548: nohA - Qin prophage; predicted packaging protein (RefSeq), at 1,633,864 to 1,634,391 nohA b4533: ynfO - Qin prophage; predicted protein (NCBI), at 1,634,780 to 1,635,013 ynfO b1549: ydfO - orf, hypothetical protein (VIMSS), at 1,635,071 to 1,635,481 ydfO Position (kb) 1633 1634 1635Strain fitness (log2 ratio) -1 0 1 2at 1632.995 kb on + strandat 1633.092 kb on - strand, within stfQat 1633.234 kb on + strand, within stfQat 1633.234 kb on + strand, within stfQat 1633.282 kb on - strand, within stfQat 1633.282 kb on - strand, within stfQat 1633.282 kb on - strand, within stfQat 1633.716 kb on + strand, within stfQat 1633.724 kb on - strand, within stfQat 1633.724 kb on - strand, within stfQat 1633.829 kb on - strandat 1634.153 kb on + strand, within nohAat 1634.153 kb on + strand, within nohAat 1634.884 kb on + strand, within ynfOat 1634.884 kb on + strand, within ynfOat 1634.916 kb on + strand, within ynfOat 1634.916 kb on + strand, within ynfO

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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1,632,995 + +0.3
1,633,092 - stfQ b1547 0.19 -0.1
1,633,234 + stfQ b1547 0.34 +0.6
1,633,234 + stfQ b1547 0.34 +0.9
1,633,282 - stfQ b1547 0.39 +0.5
1,633,282 - stfQ b1547 0.39 -0.4
1,633,282 - stfQ b1547 0.39 -0.0
1,633,716 + stfQ b1547 0.84 -1.2
1,633,724 - stfQ b1547 0.85 -1.4
1,633,724 - stfQ b1547 0.85 +0.7
1,633,829 - +0.7
1,634,153 + nohA b1548 0.55 +0.9
1,634,153 + nohA b1548 0.55 +0.1
1,634,884 + ynfO b4533 0.44 +0.1
1,634,884 + ynfO b4533 0.44 +0.6
1,634,916 + ynfO b4533 0.58 +2.7
1,634,916 + ynfO b4533 0.58 +0.5

Or see this region's nucleotide sequence