Strain Fitness in Escherichia coli BW25113 around b1512

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydeV and lsrR are separated by 78 nucleotideslsrR and lsrA are separated by 248 nucleotides b1511: ydeV - putative kinase (VIMSS), at 1,596,641 to 1,598,233 ydeV b1512: lsrR - lsr operon transcriptional repressor (NCBI), at 1,598,312 to 1,599,265 lsrR b1513: lsrA - fused AI2 transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 1,599,514 to 1,601,049 lsrA Position (kb) 1598 1599 1600Strain fitness (log2 ratio) -1 0 1 2at 1597.556 kb on - strand, within ydeVat 1597.602 kb on + strand, within ydeVat 1598.188 kb on - strandat 1598.354 kb on - strandat 1598.354 kb on - strandat 1598.443 kb on + strand, within lsrRat 1598.582 kb on + strand, within lsrRat 1598.950 kb on + strand, within lsrRat 1598.950 kb on + strand, within lsrRat 1598.997 kb on - strand, within lsrRat 1599.098 kb on - strand, within lsrRat 1599.110 kb on - strand, within lsrRat 1599.478 kb on + strandat 1599.478 kb on + strandat 1599.628 kb on + strandat 1599.697 kb on - strand, within lsrAat 1599.697 kb on - strand, within lsrAat 1599.928 kb on - strand, within lsrAat 1599.928 kb on - strand, within lsrA

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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1,597,556 - ydeV b1511 0.57 +0.7
1,597,602 + ydeV b1511 0.60 +0.8
1,598,188 - +0.3
1,598,354 - +0.5
1,598,354 - -0.4
1,598,443 + lsrR b1512 0.14 +0.7
1,598,582 + lsrR b1512 0.28 +0.7
1,598,950 + lsrR b1512 0.67 -1.5
1,598,950 + lsrR b1512 0.67 -1.3
1,598,997 - lsrR b1512 0.72 -0.0
1,599,098 - lsrR b1512 0.82 +0.5
1,599,110 - lsrR b1512 0.84 +0.3
1,599,478 + +0.0
1,599,478 + +2.1
1,599,628 + -0.4
1,599,697 - lsrA b1513 0.12 +0.1
1,599,697 - lsrA b1513 0.12 +0.2
1,599,928 - lsrA b1513 0.27 -0.3
1,599,928 - lsrA b1513 0.27 +0.1

Or see this region's nucleotide sequence