Strain Fitness in Escherichia coli BW25113 around b1372

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntinsH-5 and b1372 are separated by 322 nucleotidesb1372 and tfaR overlap by 1 nucleotidestfaR and pinR are separated by 97 nucleotides b1370: insH-5 - Rac prophage; IS5 transposase and trans-activator (NCBI), at 1,425,770 to 1,426,750 insH-5 b1372: b1372 - putative membrane protein (VIMSS), at 1,427,073 to 1,430,435 b1372 b1373: tfaR - Rac prophage; predicted tail fiber assembly protein (NCBI), at 1,430,435 to 1,431,010 tfaR b1374: pinR - Rac prophage; predicted site-specific recombinase (NCBI), at 1,431,108 to 1,431,698 pinR Position (kb) 1427 1428 1429 1430 1431Strain fitness (log2 ratio) -2 -1 0 1 2at 1426.405 kb on + strand, within insH-5at 1426.456 kb on - strand, within insH-5at 1426.658 kb on + strandat 1426.658 kb on + strandat 1426.738 kb on + strandat 1426.738 kb on + strandat 1426.746 kb on + strandat 1426.877 kb on + strandat 1426.954 kb on - strandat 1426.954 kb on - strandat 1427.144 kb on - strandat 1427.251 kb on - strandat 1427.764 kb on - strand, within b1372at 1427.764 kb on - strand, within b1372at 1427.937 kb on + strand, within b1372at 1428.125 kb on + strand, within b1372at 1428.482 kb on + strand, within b1372at 1428.485 kb on - strand, within b1372at 1428.485 kb on - strand, within b1372at 1428.506 kb on + strand, within b1372at 1428.633 kb on - strand, within b1372at 1428.633 kb on - strand, within b1372at 1428.681 kb on - strand, within b1372at 1428.681 kb on - strand, within b1372at 1428.751 kb on + strand, within b1372at 1428.807 kb on - strand, within b1372at 1428.972 kb on + strand, within b1372at 1429.180 kb on - strand, within b1372at 1429.180 kb on - strand, within b1372at 1429.248 kb on + strand, within b1372at 1429.418 kb on + strand, within b1372at 1429.429 kb on + strand, within b1372at 1429.429 kb on + strand, within b1372at 1429.526 kb on - strand, within b1372at 1429.542 kb on - strand, within b1372at 1429.679 kb on + strand, within b1372at 1429.832 kb on - strand, within b1372at 1430.281 kb on + strandat 1431.253 kb on - strand, within pinR

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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1,426,405 + insH-5 b1370 0.65 +0.0
1,426,456 - insH-5 b1370 0.70 -0.1
1,426,658 + -0.0
1,426,658 + -0.4
1,426,738 + +0.2
1,426,738 + +2.5
1,426,746 + +0.3
1,426,877 + -0.3
1,426,954 - -0.3
1,426,954 - +2.1
1,427,144 - +0.1
1,427,251 - -0.1
1,427,764 - b1372 b1372 0.21 +0.5
1,427,764 - b1372 b1372 0.21 -1.3
1,427,937 + b1372 b1372 0.26 +0.5
1,428,125 + b1372 b1372 0.31 +0.4
1,428,482 + b1372 b1372 0.42 +0.5
1,428,485 - b1372 b1372 0.42 +0.4
1,428,485 - b1372 b1372 0.42 +0.2
1,428,506 + b1372 b1372 0.43 +0.5
1,428,633 - b1372 b1372 0.46 +0.3
1,428,633 - b1372 b1372 0.46 -0.1
1,428,681 - b1372 b1372 0.48 -1.4
1,428,681 - b1372 b1372 0.48 -0.2
1,428,751 + b1372 b1372 0.50 +0.4
1,428,807 - b1372 b1372 0.52 +0.8
1,428,972 + b1372 b1372 0.56 -0.2
1,429,180 - b1372 b1372 0.63 +0.2
1,429,180 - b1372 b1372 0.63 +0.2
1,429,248 + b1372 b1372 0.65 -1.1
1,429,418 + b1372 b1372 0.70 +1.2
1,429,429 + b1372 b1372 0.70 +0.6
1,429,429 + b1372 b1372 0.70 +1.5
1,429,526 - b1372 b1372 0.73 +0.2
1,429,542 - b1372 b1372 0.73 -1.8
1,429,679 + b1372 b1372 0.77 +0.6
1,429,832 - b1372 b1372 0.82 +1.1
1,430,281 + +0.2
1,431,253 - pinR b1374 0.25 -0.2

Or see this region's nucleotide sequence