Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mrdA and ybeA are separated by 30 nucleotides ybeA and ybeB are separated by 3 nucleotides ybeB and cobC are separated by 259 nucleotides
b0635: mrdA - transpeptidase involved in peptidoglycan synthesis (penicillin-binding protein 2) (NCBI), at 665,539 to 667,440
mrdA
b0636: ybeA - hypothetical protein (NCBI), at 667,471 to 667,938
ybeA
b0637: ybeB - orf, hypothetical protein (VIMSS), at 667,942 to 668,259
ybeB
b0638: cobC - predicted alpha-ribazole-5'-P phosphatase (NCBI), at 668,519 to 669,130
cobC
Position (kb) 667
668 Strain fitness (log2 ratio) -1
0
1 at 667.444 kb on - strand at 667.495 kb on - strand at 667.495 kb on - strand at 667.495 kb on - strand at 667.524 kb on - strand, within ybeA at 667.524 kb on - strand, within ybeA at 667.588 kb on - strand, within ybeA at 667.590 kb on - strand, within ybeA at 667.665 kb on - strand, within ybeA at 667.665 kb on - strand, within ybeA at 667.703 kb on - strand, within ybeA at 667.703 kb on - strand, within ybeA at 667.762 kb on - strand, within ybeA at 667.762 kb on - strand, within ybeA at 667.852 kb on - strand, within ybeA at 667.852 kb on - strand, within ybeA at 668.424 kb on + strand at 668.466 kb on - strand at 668.466 kb on - strand at 668.601 kb on - strand, within cobC at 668.672 kb on + strand, within cobC at 668.680 kb on - strand, within cobC at 668.733 kb on + strand, within cobC at 668.733 kb on + strand, within cobC at 668.796 kb on - strand, within cobC at 668.796 kb on - strand, within cobC at 668.930 kb on + strand, within cobC
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 667,444 - -1.1 667,495 - -0.4 667,495 - -0.3 667,495 - +1.1 667,524 - ybeA b0636 0.11 -0.4 667,524 - ybeA b0636 0.11 -0.8 667,588 - ybeA b0636 0.25 -0.1 667,590 - ybeA b0636 0.25 -1.2 667,665 - ybeA b0636 0.41 -0.3 667,665 - ybeA b0636 0.41 +0.1 667,703 - ybeA b0636 0.50 -0.8 667,703 - ybeA b0636 0.50 -1.4 667,762 - ybeA b0636 0.62 -0.6 667,762 - ybeA b0636 0.62 +0.5 667,852 - ybeA b0636 0.81 -0.6 667,852 - ybeA b0636 0.81 -0.8 668,424 + +0.5 668,466 - +0.2 668,466 - -0.3 668,601 - cobC b0638 0.13 +0.5 668,672 + cobC b0638 0.25 +0.2 668,680 - cobC b0638 0.26 +0.3 668,733 + cobC b0638 0.35 -0.3 668,733 + cobC b0638 0.35 -0.7 668,796 - cobC b0638 0.45 -1.1 668,796 - cobC b0638 0.45 +1.0 668,930 + cobC b0638 0.67 +0.1
Or see this region's nucleotide sequence