Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt proA and thrW are separated by 114 nucleotides thrW and ykfN are separated by 203 nucleotides ykfN and ykfI are separated by 115 nucleotides ykfI and yafW are separated by 20 nucleotides yafW and ykfH are separated by 18 nucleotides ykfH and ykfG are separated by 8 nucleotides
b0243: proA - gamma-glutamyl phosphate reductase (NCBI), at 260,727 to 261,980
proA
b0244: thrW - tRNA-Thr (NCBI), at 262,095 to 262,170
thrW
b4626: ykfN - no description, at 262,374 to 262,436
ykfN
b0245: ykfI - CP4-6 prophage; toxin of the YkfI-YafW toxin-antitoxin system (NCBI), at 262,552 to 262,893
ykfI
b0246: yafW - CP4-6 prophage; antitoxin of the YkfI-YafW toxin-antitoxin system (NCBI), at 262,914 to 263,231
yafW
b4504: ykfH - hypothetical protein (NCBI), at 263,250 to 263,471
ykfH
b0247: ykfG - CP4-6 prophage; predicted DNA repair protein (NCBI), at 263,480 to 263,956
ykfG
Position (kb) 262
263 Strain fitness (log2 ratio) -2
-1
0
1
2 at 261.583 kb on - strand, within proA at 261.583 kb on - strand, within proA at 261.605 kb on + strand, within proA at 261.656 kb on - strand, within proA at 261.670 kb on - strand, within proA at 261.670 kb on - strand, within proA at 261.746 kb on + strand, within proA at 261.759 kb on - strand, within proA at 261.759 kb on - strand, within proA at 261.824 kb on - strand, within proA at 261.824 kb on - strand, within proA at 261.869 kb on - strand at 261.916 kb on - strand at 261.916 kb on - strand at 261.960 kb on - strand at 261.960 kb on - strand at 262.045 kb on + strand at 262.091 kb on - strand at 262.113 kb on + strand, within thrW at 262.270 kb on + strand at 262.310 kb on + strand at 262.310 kb on + strand at 262.319 kb on + strand at 262.371 kb on + strand at 262.479 kb on - strand at 262.546 kb on - strand at 262.548 kb on + strand at 262.565 kb on + strand at 262.644 kb on - strand, within ykfI at 262.717 kb on - strand, within ykfI at 262.719 kb on + strand, within ykfI at 262.727 kb on - strand, within ykfI at 262.727 kb on - strand, within ykfI at 262.727 kb on - strand, within ykfI at 262.730 kb on + strand, within ykfI at 262.730 kb on + strand, within ykfI at 262.738 kb on - strand, within ykfI at 262.760 kb on + strand, within ykfI at 262.786 kb on - strand, within ykfI at 262.909 kb on - strand at 262.909 kb on - strand at 262.917 kb on + strand at 262.917 kb on + strand at 262.917 kb on + strand at 262.935 kb on - strand at 262.943 kb on + strand at 262.943 kb on + strand at 262.980 kb on + strand, within yafW at 262.980 kb on + strand, within yafW at 262.994 kb on + strand, within yafW at 262.998 kb on - strand, within yafW at 263.008 kb on - strand, within yafW at 263.008 kb on - strand, within yafW at 263.078 kb on - strand, within yafW at 263.151 kb on - strand, within yafW at 263.169 kb on + strand, within yafW at 263.192 kb on + strand, within yafW at 263.226 kb on + strand at 263.234 kb on - strand at 263.234 kb on - strand at 263.234 kb on - strand at 263.273 kb on + strand, within ykfH at 263.278 kb on + strand, within ykfH at 263.365 kb on - strand, within ykfH at 263.393 kb on - strand, within ykfH at 263.486 kb on - strand at 263.567 kb on + strand, within ykfG
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 261,583 - proA b0243 0.68 +0.8 261,583 - proA b0243 0.68 -0.2 261,605 + proA b0243 0.70 -1.0 261,656 - proA b0243 0.74 -0.3 261,670 - proA b0243 0.75 +1.2 261,670 - proA b0243 0.75 -0.0 261,746 + proA b0243 0.81 -0.1 261,759 - proA b0243 0.82 -0.2 261,759 - proA b0243 0.82 +0.2 261,824 - proA b0243 0.87 -0.0 261,824 - proA b0243 0.87 +1.0 261,869 - -2.2 261,916 - +0.9 261,916 - -1.7 261,960 - +0.6 261,960 - -0.8 262,045 + +0.6 262,091 - +0.3 262,113 + thrW b0244 0.24 +1.8 262,270 + -0.6 262,310 + -0.1 262,310 + -0.5 262,319 + +0.4 262,371 + -0.1 262,479 - +0.2 262,546 - +0.4 262,548 + -0.0 262,565 + +0.4 262,644 - ykfI b0245 0.27 +0.2 262,717 - ykfI b0245 0.48 +0.5 262,719 + ykfI b0245 0.49 +0.0 262,727 - ykfI b0245 0.51 +0.6 262,727 - ykfI b0245 0.51 +0.4 262,727 - ykfI b0245 0.51 +0.5 262,730 + ykfI b0245 0.52 -0.2 262,730 + ykfI b0245 0.52 +0.6 262,738 - ykfI b0245 0.54 -0.1 262,760 + ykfI b0245 0.61 -0.2 262,786 - ykfI b0245 0.68 +0.9 262,909 - +0.2 262,909 - +0.1 262,917 + -0.7 262,917 + -0.9 262,917 + +0.9 262,935 - -1.9 262,943 + +1.8 262,943 + -0.3 262,980 + yafW b0246 0.21 -1.0 262,980 + yafW b0246 0.21 +0.4 262,994 + yafW b0246 0.25 +0.2 262,998 - yafW b0246 0.26 +0.0 263,008 - yafW b0246 0.30 -0.1 263,008 - yafW b0246 0.30 -0.1 263,078 - yafW b0246 0.52 +1.3 263,151 - yafW b0246 0.75 +1.0 263,169 + yafW b0246 0.80 -0.1 263,192 + yafW b0246 0.87 +0.8 263,226 + +0.5 263,234 - +0.3 263,234 - -0.0 263,234 - +0.2 263,273 + ykfH b4504 0.10 -0.1 263,278 + ykfH b4504 0.13 -0.4 263,365 - ykfH b4504 0.52 +0.1 263,393 - ykfH b4504 0.64 -0.1 263,486 - +0.2 263,567 + ykfG b0247 0.18 -0.4
Or see this region's nucleotide sequence