Strain Fitness in Escherichia coli BW25113 around b3271

Experiment: Cisplatin 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyhdY and yhdZ are separated by 7 nucleotidesyhdZ and rrfF are separated by 228 nucleotidesrrfF and thrV are separated by 37 nucleotidesthrV and rrfD are separated by 12 nucleotidesrrfD and rrlD are separated by 92 nucleotides b3270: yhdY - putative transport system permease protein (VIMSS), at 3,419,347 to 3,420,450 yhdY b3271: yhdZ - predicted amino-acid transporter subunit (NCBI), at 3,420,458 to 3,421,216 yhdZ b3272: rrfF - 5S ribosomal RNA (NCBI), at 3,421,445 to 3,421,564 rrfF b3273: thrV - tRNA-Thr (NCBI), at 3,421,602 to 3,421,677 thrV b3274: rrfD - 5S ribosomal RNA (NCBI), at 3,421,690 to 3,421,809 rrfD b3275: rrlD - 23S ribosomal RNA (NCBI), at 3,421,902 to 3,424,805 rrlD Position (kb) 3420 3421 3422Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3419.462 kb on - strand, within yhdYat 3419.462 kb on - strand, within yhdYat 3419.500 kb on + strand, within yhdYat 3419.578 kb on + strand, within yhdYat 3419.578 kb on + strand, within yhdYat 3419.578 kb on + strand, within yhdYat 3419.580 kb on - strand, within yhdYat 3419.580 kb on - strand, within yhdYat 3419.671 kb on + strand, within yhdYat 3419.671 kb on + strand, within yhdYat 3419.671 kb on + strand, within yhdYat 3419.671 kb on + strand, within yhdYat 3419.674 kb on - strand, within yhdYat 3419.674 kb on - strand, within yhdYat 3419.741 kb on + strand, within yhdYat 3419.805 kb on - strand, within yhdYat 3419.814 kb on + strand, within yhdYat 3419.868 kb on + strand, within yhdYat 3419.881 kb on + strand, within yhdYat 3419.911 kb on - strand, within yhdYat 3419.911 kb on - strand, within yhdYat 3419.979 kb on + strand, within yhdYat 3420.064 kb on - strand, within yhdYat 3420.083 kb on - strand, within yhdYat 3420.117 kb on + strand, within yhdYat 3420.256 kb on + strand, within yhdYat 3420.306 kb on + strand, within yhdYat 3420.423 kb on + strandat 3420.423 kb on + strandat 3420.431 kb on - strandat 3420.434 kb on + strandat 3420.434 kb on + strandat 3420.627 kb on + strand, within yhdZat 3420.627 kb on + strand, within yhdZat 3420.734 kb on + strand, within yhdZat 3420.764 kb on - strand, within yhdZat 3420.786 kb on - strand, within yhdZat 3420.846 kb on + strand, within yhdZat 3420.849 kb on + strand, within yhdZat 3420.857 kb on - strand, within yhdZat 3420.857 kb on - strand, within yhdZat 3420.857 kb on - strand, within yhdZat 3420.924 kb on + strand, within yhdZat 3421.053 kb on - strand, within yhdZat 3421.176 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml
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3,419,462 - yhdY b3270 0.10 -1.2
3,419,462 - yhdY b3270 0.10 -2.9
3,419,500 + yhdY b3270 0.14 -0.1
3,419,578 + yhdY b3270 0.21 +0.7
3,419,578 + yhdY b3270 0.21 -0.3
3,419,578 + yhdY b3270 0.21 -0.2
3,419,580 - yhdY b3270 0.21 +1.8
3,419,580 - yhdY b3270 0.21 -0.2
3,419,671 + yhdY b3270 0.29 -0.3
3,419,671 + yhdY b3270 0.29 -0.2
3,419,671 + yhdY b3270 0.29 +0.4
3,419,671 + yhdY b3270 0.29 -0.2
3,419,674 - yhdY b3270 0.30 +1.7
3,419,674 - yhdY b3270 0.30 -0.1
3,419,741 + yhdY b3270 0.36 -1.5
3,419,805 - yhdY b3270 0.41 +1.0
3,419,814 + yhdY b3270 0.42 -0.4
3,419,868 + yhdY b3270 0.47 -1.1
3,419,881 + yhdY b3270 0.48 -0.2
3,419,911 - yhdY b3270 0.51 -0.3
3,419,911 - yhdY b3270 0.51 -0.2
3,419,979 + yhdY b3270 0.57 -0.1
3,420,064 - yhdY b3270 0.65 +0.3
3,420,083 - yhdY b3270 0.67 +0.2
3,420,117 + yhdY b3270 0.70 -2.7
3,420,256 + yhdY b3270 0.82 -0.9
3,420,306 + yhdY b3270 0.87 -0.2
3,420,423 + +0.1
3,420,423 + +1.4
3,420,431 - -0.1
3,420,434 + -0.5
3,420,434 + -0.6
3,420,627 + yhdZ b3271 0.22 +0.1
3,420,627 + yhdZ b3271 0.22 -0.5
3,420,734 + yhdZ b3271 0.36 +1.0
3,420,764 - yhdZ b3271 0.40 +1.0
3,420,786 - yhdZ b3271 0.43 +0.6
3,420,846 + yhdZ b3271 0.51 +0.3
3,420,849 + yhdZ b3271 0.52 +1.0
3,420,857 - yhdZ b3271 0.53 +2.7
3,420,857 - yhdZ b3271 0.53 -1.2
3,420,857 - yhdZ b3271 0.53 -0.9
3,420,924 + yhdZ b3271 0.61 +2.4
3,421,053 - yhdZ b3271 0.78 -0.6
3,421,176 - +1.2

Or see this region's nucleotide sequence