Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyfH and hyfI overlap by 4 nucleotides hyfI and b2490 overlap by 8 nucleotides b2490 and hyfR are separated by 29 nucleotides
b2488: hyfH - hydrogenase 4, Fe-S subunit (NCBI), at 2,608,186 to 2,608,731
hyfH
b2489: hyfI - hydrogenase 4, Fe-S subunit (NCBI), at 2,608,728 to 2,609,486
hyfI
b2490: b2490 - putative protein processing element (VIMSS), at 2,609,479 to 2,609,892
b2490
b2491: hyfR - putative 2-component regulator, interaction with sigma 54 (VIMSS), at 2,609,922 to 2,611,934
hyfR
Position (kb)
2609
2610 Strain fitness (log2 ratio)
-1
0
1 at 2608.658 kb on - strand, within hyfH at 2608.658 kb on - strand, within hyfH at 2608.733 kb on - strand at 2608.733 kb on - strand at 2608.757 kb on - strand at 2609.013 kb on + strand, within hyfI at 2609.013 kb on + strand, within hyfI at 2609.095 kb on - strand, within hyfI at 2609.097 kb on + strand, within hyfI at 2609.290 kb on - strand, within hyfI at 2609.329 kb on + strand, within hyfI at 2609.329 kb on + strand, within hyfI at 2609.393 kb on - strand, within hyfI at 2609.393 kb on - strand, within hyfI at 2609.480 kb on + strand at 2609.482 kb on + strand at 2609.490 kb on - strand at 2609.490 kb on - strand at 2609.576 kb on - strand, within b2490 at 2609.576 kb on - strand, within b2490 at 2609.595 kb on - strand, within b2490 at 2609.671 kb on - strand, within b2490 at 2610.075 kb on - strand at 2610.080 kb on - strand at 2610.149 kb on - strand, within hyfR at 2610.261 kb on + strand, within hyfR at 2610.284 kb on - strand, within hyfR at 2610.286 kb on - strand, within hyfR at 2610.406 kb on + strand, within hyfR at 2610.411 kb on + strand, within hyfR at 2610.555 kb on + strand, within hyfR at 2610.555 kb on + strand, within hyfR at 2610.733 kb on - strand, within hyfR at 2610.768 kb on - strand, within hyfR at 2610.768 kb on - strand, within hyfR at 2610.852 kb on - strand, within hyfR at 2610.852 kb on - strand, within hyfR
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 2,608,658 - hyfH b2488 0.86 -0.3 2,608,658 - hyfH b2488 0.86 +1.5 2,608,733 - -0.1 2,608,733 - -0.9 2,608,757 - +0.2 2,609,013 + hyfI b2489 0.38 -0.0 2,609,013 + hyfI b2489 0.38 +0.3 2,609,095 - hyfI b2489 0.48 +0.1 2,609,097 + hyfI b2489 0.49 +0.7 2,609,290 - hyfI b2489 0.74 +0.5 2,609,329 + hyfI b2489 0.79 +1.1 2,609,329 + hyfI b2489 0.79 -0.1 2,609,393 - hyfI b2489 0.88 +0.5 2,609,393 - hyfI b2489 0.88 -0.0 2,609,480 + +0.1 2,609,482 + -0.4 2,609,490 - +0.3 2,609,490 - +0.4 2,609,576 - b2490 b2490 0.23 -0.7 2,609,576 - b2490 b2490 0.23 +0.7 2,609,595 - b2490 b2490 0.28 +1.5 2,609,671 - b2490 b2490 0.46 +0.4 2,610,075 - -0.5 2,610,080 - +0.6 2,610,149 - hyfR b2491 0.11 +0.7 2,610,261 + hyfR b2491 0.17 +1.1 2,610,284 - hyfR b2491 0.18 +1.3 2,610,286 - hyfR b2491 0.18 -0.4 2,610,406 + hyfR b2491 0.24 -0.9 2,610,411 + hyfR b2491 0.24 +1.5 2,610,555 + hyfR b2491 0.31 +0.4 2,610,555 + hyfR b2491 0.31 -0.3 2,610,733 - hyfR b2491 0.40 -0.8 2,610,768 - hyfR b2491 0.42 -1.0 2,610,768 - hyfR b2491 0.42 +0.3 2,610,852 - hyfR b2491 0.46 -1.1 2,610,852 - hyfR b2491 0.46 +0.1
Or see this region's nucleotide sequence