Strain Fitness in Escherichia coli BW25113 around b2283

Experiment: Cisplatin 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnuoI and nuoH are separated by 14 nucleotidesnuoH and nuoG overlap by 4 nucleotidesnuoG and nuoF are separated by 52 nucleotides b2281: nuoI - NADH dehydrogenase subunit I (NCBI), at 2,393,930 to 2,394,472 nuoI b2282: nuoH - NADH dehydrogenase subunit H (NCBI), at 2,394,487 to 2,395,464 nuoH b2283: nuoG - NADH:ubiquinone oxidoreductase, chain G (RefSeq), at 2,395,461 to 2,398,187 nuoG b2284: nuoF - NADH:ubiquinone oxidoreductase, chain F (NCBI), at 2,398,240 to 2,399,577 nuoF Position (kb) 2395 2396 2397 2398 2399Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2394.482 kb on - strandat 2394.495 kb on - strandat 2394.495 kb on - strandat 2394.603 kb on + strand, within nuoHat 2394.603 kb on + strand, within nuoHat 2394.624 kb on + strand, within nuoHat 2394.634 kb on - strand, within nuoHat 2394.712 kb on - strandat 2394.804 kb on + strand, within nuoHat 2394.815 kb on + strand, within nuoHat 2394.834 kb on + strand, within nuoHat 2395.011 kb on + strand, within nuoHat 2395.154 kb on - strand, within nuoHat 2395.213 kb on + strand, within nuoHat 2395.213 kb on + strand, within nuoHat 2395.265 kb on + strand, within nuoHat 2395.375 kb on - strandat 2395.392 kb on - strandat 2395.406 kb on + strandat 2395.415 kb on - strandat 2395.451 kb on - strandat 2395.471 kb on + strandat 2395.711 kb on - strandat 2395.734 kb on + strand, within nuoGat 2395.793 kb on - strand, within nuoGat 2395.878 kb on + strand, within nuoGat 2395.916 kb on - strand, within nuoGat 2395.991 kb on - strandat 2396.001 kb on - strand, within nuoGat 2396.059 kb on + strand, within nuoGat 2396.067 kb on - strand, within nuoGat 2396.188 kb on + strand, within nuoGat 2396.228 kb on + strand, within nuoGat 2396.367 kb on - strand, within nuoGat 2396.424 kb on - strand, within nuoGat 2396.464 kb on - strand, within nuoGat 2396.492 kb on + strand, within nuoGat 2396.492 kb on + strandat 2396.570 kb on - strand, within nuoGat 2396.609 kb on + strand, within nuoGat 2396.697 kb on - strand, within nuoGat 2396.697 kb on - strand, within nuoGat 2396.780 kb on + strand, within nuoGat 2396.788 kb on - strand, within nuoGat 2396.793 kb on - strand, within nuoGat 2396.853 kb on - strand, within nuoGat 2396.853 kb on - strand, within nuoGat 2396.890 kb on - strand, within nuoGat 2396.914 kb on - strand, within nuoGat 2396.914 kb on - strand, within nuoGat 2396.919 kb on - strand, within nuoGat 2396.995 kb on - strand, within nuoGat 2397.033 kb on + strand, within nuoGat 2397.046 kb on - strand, within nuoGat 2397.046 kb on - strand, within nuoGat 2397.108 kb on - strand, within nuoGat 2397.147 kb on + strand, within nuoGat 2397.147 kb on + strand, within nuoGat 2397.147 kb on + strand, within nuoGat 2397.147 kb on + strand, within nuoGat 2397.180 kb on - strand, within nuoGat 2397.180 kb on - strand, within nuoGat 2397.247 kb on - strand, within nuoGat 2397.247 kb on - strand, within nuoGat 2397.264 kb on - strand, within nuoGat 2397.522 kb on + strand, within nuoGat 2397.522 kb on + strand, within nuoGat 2397.772 kb on - strand, within nuoGat 2397.785 kb on + strand, within nuoGat 2398.076 kb on - strandat 2398.076 kb on - strandat 2398.222 kb on - strandat 2398.338 kb on + strandat 2398.349 kb on + strandat 2398.351 kb on + strandat 2398.351 kb on + strandat 2398.359 kb on - strandat 2398.367 kb on + strandat 2398.449 kb on + strand, within nuoFat 2398.487 kb on + strand, within nuoFat 2398.570 kb on + strand, within nuoFat 2398.573 kb on - strand, within nuoFat 2398.610 kb on + strand, within nuoFat 2398.770 kb on + strand, within nuoFat 2398.778 kb on + strand, within nuoFat 2398.795 kb on - strand, within nuoFat 2398.831 kb on - strand, within nuoFat 2398.874 kb on - strand, within nuoFat 2398.876 kb on + strand, within nuoFat 2398.883 kb on + strand, within nuoFat 2398.914 kb on + strand, within nuoFat 2398.914 kb on + strandat 2398.964 kb on + strand, within nuoFat 2398.964 kb on + strand, within nuoFat 2399.113 kb on - strand, within nuoF

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml
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2,394,482 - +1.7
2,394,495 - -0.4
2,394,495 - -3.0
2,394,603 + nuoH b2282 0.12 +2.1
2,394,603 + nuoH b2282 0.12 +0.7
2,394,624 + nuoH b2282 0.14 +0.7
2,394,634 - nuoH b2282 0.15 +0.4
2,394,712 - +0.9
2,394,804 + nuoH b2282 0.32 +0.6
2,394,815 + nuoH b2282 0.34 -1.1
2,394,834 + nuoH b2282 0.35 +0.5
2,395,011 + nuoH b2282 0.54 +2.4
2,395,154 - nuoH b2282 0.68 -0.1
2,395,213 + nuoH b2282 0.74 +0.1
2,395,213 + nuoH b2282 0.74 -1.1
2,395,265 + nuoH b2282 0.80 -1.7
2,395,375 - +1.1
2,395,392 - +1.4
2,395,406 + +2.1
2,395,415 - +1.8
2,395,451 - +0.9
2,395,471 + -0.7
2,395,711 - -0.7
2,395,734 + nuoG b2283 0.10 +0.5
2,395,793 - nuoG b2283 0.12 +2.4
2,395,878 + nuoG b2283 0.15 -0.1
2,395,916 - nuoG b2283 0.17 +0.7
2,395,991 - +1.5
2,396,001 - nuoG b2283 0.20 +2.1
2,396,059 + nuoG b2283 0.22 +2.0
2,396,067 - nuoG b2283 0.22 -0.1
2,396,188 + nuoG b2283 0.27 +2.8
2,396,228 + nuoG b2283 0.28 +0.7
2,396,367 - nuoG b2283 0.33 -1.6
2,396,424 - nuoG b2283 0.35 -0.9
2,396,464 - nuoG b2283 0.37 +0.1
2,396,492 + nuoG b2283 0.38 +1.9
2,396,492 + -0.1
2,396,570 - nuoG b2283 0.41 -1.9
2,396,609 + nuoG b2283 0.42 +0.1
2,396,697 - nuoG b2283 0.45 +2.3
2,396,697 - nuoG b2283 0.45 +2.6
2,396,780 + nuoG b2283 0.48 +0.2
2,396,788 - nuoG b2283 0.49 -0.1
2,396,793 - nuoG b2283 0.49 +1.1
2,396,853 - nuoG b2283 0.51 +1.9
2,396,853 - nuoG b2283 0.51 -1.9
2,396,890 - nuoG b2283 0.52 +1.6
2,396,914 - nuoG b2283 0.53 +1.1
2,396,914 - nuoG b2283 0.53 -1.4
2,396,919 - nuoG b2283 0.53 -0.6
2,396,995 - nuoG b2283 0.56 -0.7
2,397,033 + nuoG b2283 0.58 +2.1
2,397,046 - nuoG b2283 0.58 +0.9
2,397,046 - nuoG b2283 0.58 -2.3
2,397,108 - nuoG b2283 0.60 +1.9
2,397,147 + nuoG b2283 0.62 +2.2
2,397,147 + nuoG b2283 0.62 +0.9
2,397,147 + nuoG b2283 0.62 +0.1
2,397,147 + nuoG b2283 0.62 +0.4
2,397,180 - nuoG b2283 0.63 +0.6
2,397,180 - nuoG b2283 0.63 +0.1
2,397,247 - nuoG b2283 0.65 +0.5
2,397,247 - nuoG b2283 0.65 +2.1
2,397,264 - nuoG b2283 0.66 -0.1
2,397,522 + nuoG b2283 0.76 -0.7
2,397,522 + nuoG b2283 0.76 +1.4
2,397,772 - nuoG b2283 0.85 +1.1
2,397,785 + nuoG b2283 0.85 +0.4
2,398,076 - +1.7
2,398,076 - -1.1
2,398,222 - +1.9
2,398,338 + +1.5
2,398,349 + +1.6
2,398,351 + +1.5
2,398,351 + +2.2
2,398,359 - +0.9
2,398,367 + -0.4
2,398,449 + nuoF b2284 0.16 +2.3
2,398,487 + nuoF b2284 0.18 -0.9
2,398,570 + nuoF b2284 0.25 +1.8
2,398,573 - nuoF b2284 0.25 +0.5
2,398,610 + nuoF b2284 0.28 +0.1
2,398,770 + nuoF b2284 0.40 +0.1
2,398,778 + nuoF b2284 0.40 +2.0
2,398,795 - nuoF b2284 0.41 -1.1
2,398,831 - nuoF b2284 0.44 -1.4
2,398,874 - nuoF b2284 0.47 +0.1
2,398,876 + nuoF b2284 0.48 +1.5
2,398,883 + nuoF b2284 0.48 -0.1
2,398,914 + nuoF b2284 0.50 +0.9
2,398,914 + +3.5
2,398,964 + nuoF b2284 0.54 +0.9
2,398,964 + nuoF b2284 0.54 +2.5
2,399,113 - nuoF b2284 0.65 +0.5

Or see this region's nucleotide sequence