Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pphA and ryeA are separated by 96 nucleotides ryeA and ryeB overlap by 151 nucleotides ryeB and yebY are separated by 80 nucleotides yebY and yebZ are separated by 12 nucleotides yebZ and yobA are separated by 3 nucleotides yobA and holE are separated by 138 nucleotides holE and yobB are separated by 101 nucleotides
b1838: pphA - protein phosphatase 1 modulates phosphoproteins, signals protein misfolding (VIMSS), at 1,920,337 to 1,920,993
pphA
b4432: ryeA - unknown RNA (NCBI), at 1,921,090 to 1,921,338
ryeA
b4433: ryeB - unknown RNA (NCBI), at 1,921,188 to 1,921,308
ryeB
b1839: yebY - hypothetical protein (NCBI), at 1,921,389 to 1,921,730
yebY
b1840: yebZ - predicted inner membrane protein (NCBI), at 1,921,743 to 1,922,615
yebZ
b1841: yobA - hypothetical protein (NCBI), at 1,922,619 to 1,922,993
yobA
b1842: holE - DNA polymerase III, theta subunit (NCBI), at 1,923,132 to 1,923,362
holE
b1843: yobB - hypothetical protein (NCBI), at 1,923,464 to 1,924,120
yobB
Position (kb)
1921
1922
1923 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1920.775 kb on + strand, within pphA at 1920.775 kb on + strand, within pphA at 1920.812 kb on - strand, within pphA at 1921.054 kb on + strand at 1921.076 kb on - strand at 1921.222 kb on - strand at 1921.222 kb on - strand at 1921.224 kb on + strand at 1921.412 kb on + strand at 1921.546 kb on + strand, within yebY at 1921.621 kb on + strand, within yebY at 1921.670 kb on + strand, within yebY at 1921.670 kb on + strand, within yebY at 1921.682 kb on + strand, within yebY at 1921.828 kb on + strand at 1921.828 kb on + strand at 1921.828 kb on + strand at 1921.828 kb on + strand at 1921.846 kb on + strand, within yebZ at 1922.093 kb on - strand, within yebZ at 1922.107 kb on - strand, within yebZ at 1922.363 kb on - strand, within yebZ at 1922.363 kb on - strand, within yebZ at 1922.430 kb on + strand, within yebZ at 1922.430 kb on + strand, within yebZ at 1922.438 kb on + strand, within yebZ at 1922.446 kb on - strand, within yebZ at 1922.482 kb on - strand, within yebZ at 1922.528 kb on + strand at 1922.536 kb on - strand at 1922.642 kb on - strand at 1922.681 kb on + strand, within yobA at 1922.681 kb on + strand, within yobA at 1923.028 kb on + strand at 1923.059 kb on - strand at 1923.246 kb on - strand, within holE at 1923.246 kb on - strand, within holE at 1923.296 kb on + strand, within holE at 1923.364 kb on + strand at 1923.364 kb on + strand at 1923.493 kb on + strand at 1923.493 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 1,920,775 + pphA b1838 0.67 +0.7 1,920,775 + pphA b1838 0.67 +0.2 1,920,812 - pphA b1838 0.72 -0.4 1,921,054 + +2.0 1,921,076 - +0.4 1,921,222 - -0.1 1,921,222 - +0.8 1,921,224 + +0.9 1,921,412 + -0.6 1,921,546 + yebY b1839 0.46 +0.4 1,921,621 + yebY b1839 0.68 +0.3 1,921,670 + yebY b1839 0.82 -3.4 1,921,670 + yebY b1839 0.82 +1.4 1,921,682 + yebY b1839 0.86 -0.2 1,921,828 + -0.2 1,921,828 + +2.2 1,921,828 + +2.1 1,921,828 + -2.0 1,921,846 + yebZ b1840 0.12 -1.2 1,922,093 - yebZ b1840 0.40 +1.5 1,922,107 - yebZ b1840 0.42 +1.7 1,922,363 - yebZ b1840 0.71 +1.0 1,922,363 - yebZ b1840 0.71 +1.8 1,922,430 + yebZ b1840 0.79 +1.1 1,922,430 + yebZ b1840 0.79 +0.7 1,922,438 + yebZ b1840 0.80 +1.4 1,922,446 - yebZ b1840 0.81 +2.0 1,922,482 - yebZ b1840 0.85 +1.9 1,922,528 + +0.0 1,922,536 - +1.4 1,922,642 - +1.7 1,922,681 + yobA b1841 0.17 +1.5 1,922,681 + yobA b1841 0.17 +0.7 1,923,028 + +1.2 1,923,059 - -0.3 1,923,246 - holE b1842 0.49 -0.7 1,923,246 - holE b1842 0.49 +1.5 1,923,296 + holE b1842 0.71 -0.1 1,923,364 + -1.2 1,923,364 + +0.3 1,923,493 + -0.1 1,923,493 + -1.5
Or see this region's nucleotide sequence