Strain Fitness in Escherichia coli BW25113 around b1840

Experiment: Cisplatin 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpphA and ryeA are separated by 96 nucleotidesryeA and ryeB overlap by 151 nucleotidesryeB and yebY are separated by 80 nucleotidesyebY and yebZ are separated by 12 nucleotidesyebZ and yobA are separated by 3 nucleotidesyobA and holE are separated by 138 nucleotidesholE and yobB are separated by 101 nucleotides b1838: pphA - protein phosphatase 1 modulates phosphoproteins, signals protein misfolding (VIMSS), at 1,920,337 to 1,920,993 pphA b4432: ryeA - unknown RNA (NCBI), at 1,921,090 to 1,921,338 ryeA b4433: ryeB - unknown RNA (NCBI), at 1,921,188 to 1,921,308 ryeB b1839: yebY - hypothetical protein (NCBI), at 1,921,389 to 1,921,730 yebY b1840: yebZ - predicted inner membrane protein (NCBI), at 1,921,743 to 1,922,615 yebZ b1841: yobA - hypothetical protein (NCBI), at 1,922,619 to 1,922,993 yobA b1842: holE - DNA polymerase III, theta subunit (NCBI), at 1,923,132 to 1,923,362 holE b1843: yobB - hypothetical protein (NCBI), at 1,923,464 to 1,924,120 yobB Position (kb) 1921 1922 1923Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1920.775 kb on + strand, within pphAat 1920.775 kb on + strand, within pphAat 1920.812 kb on - strand, within pphAat 1921.054 kb on + strandat 1921.076 kb on - strandat 1921.222 kb on - strandat 1921.222 kb on - strandat 1921.224 kb on + strandat 1921.412 kb on + strandat 1921.546 kb on + strand, within yebYat 1921.621 kb on + strand, within yebYat 1921.670 kb on + strand, within yebYat 1921.670 kb on + strand, within yebYat 1921.682 kb on + strand, within yebYat 1921.828 kb on + strandat 1921.828 kb on + strandat 1921.828 kb on + strandat 1921.828 kb on + strandat 1921.846 kb on + strand, within yebZat 1922.093 kb on - strand, within yebZat 1922.107 kb on - strand, within yebZat 1922.363 kb on - strand, within yebZat 1922.363 kb on - strand, within yebZat 1922.430 kb on + strand, within yebZat 1922.430 kb on + strand, within yebZat 1922.438 kb on + strand, within yebZat 1922.446 kb on - strand, within yebZat 1922.482 kb on - strand, within yebZat 1922.528 kb on + strandat 1922.536 kb on - strandat 1922.642 kb on - strandat 1922.681 kb on + strand, within yobAat 1922.681 kb on + strand, within yobAat 1923.028 kb on + strandat 1923.059 kb on - strandat 1923.246 kb on - strand, within holEat 1923.246 kb on - strand, within holEat 1923.296 kb on + strand, within holEat 1923.364 kb on + strandat 1923.364 kb on + strandat 1923.493 kb on + strandat 1923.493 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml
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1,920,775 + pphA b1838 0.67 +0.7
1,920,775 + pphA b1838 0.67 +0.2
1,920,812 - pphA b1838 0.72 -0.4
1,921,054 + +2.0
1,921,076 - +0.4
1,921,222 - -0.1
1,921,222 - +0.8
1,921,224 + +0.9
1,921,412 + -0.6
1,921,546 + yebY b1839 0.46 +0.4
1,921,621 + yebY b1839 0.68 +0.3
1,921,670 + yebY b1839 0.82 -3.4
1,921,670 + yebY b1839 0.82 +1.4
1,921,682 + yebY b1839 0.86 -0.2
1,921,828 + -0.2
1,921,828 + +2.2
1,921,828 + +2.1
1,921,828 + -2.0
1,921,846 + yebZ b1840 0.12 -1.2
1,922,093 - yebZ b1840 0.40 +1.5
1,922,107 - yebZ b1840 0.42 +1.7
1,922,363 - yebZ b1840 0.71 +1.0
1,922,363 - yebZ b1840 0.71 +1.8
1,922,430 + yebZ b1840 0.79 +1.1
1,922,430 + yebZ b1840 0.79 +0.7
1,922,438 + yebZ b1840 0.80 +1.4
1,922,446 - yebZ b1840 0.81 +2.0
1,922,482 - yebZ b1840 0.85 +1.9
1,922,528 + +0.0
1,922,536 - +1.4
1,922,642 - +1.7
1,922,681 + yobA b1841 0.17 +1.5
1,922,681 + yobA b1841 0.17 +0.7
1,923,028 + +1.2
1,923,059 - -0.3
1,923,246 - holE b1842 0.49 -0.7
1,923,246 - holE b1842 0.49 +1.5
1,923,296 + holE b1842 0.71 -0.1
1,923,364 + -1.2
1,923,364 + +0.3
1,923,493 + -0.1
1,923,493 + -1.5

Or see this region's nucleotide sequence