Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flxA and intK are separated by 436 nucleotides intK and ydfX are separated by 1 nucleotides ydfX and dicC overlap by 17 nucleotides dicC and dicA are separated by 83 nucleotides dicA and ydfA are separated by 166 nucleotides ydfA and ydfB are separated by 1 nucleotides ydfB and ydfC are separated by 29 nucleotides
b1566: flxA - Qin prophage; predicted protein (NCBI), at 1,644,429 to 1,644,761
flxA
b1567: intK - Qin prophage; predicted protein (RefSeq), at 1,645,198 to 1,645,380
intK
b1568: ydfX - Qin prophage; predicted protein (RefSeq), at 1,645,382 to 1,645,660
ydfX
b1569: dicC - Qin prophage; DNA-binding transcriptional regulator for DicB (NCBI), at 1,645,644 to 1,645,874
dicC
b1570: dicA - Qin prophage; predicted regulator for DicB (NCBI), at 1,645,958 to 1,646,365
dicA
b1571: ydfA - Qin prophage; predicted protein (NCBI), at 1,646,532 to 1,646,687
ydfA
b1572: ydfB - orf, hypothetical protein (VIMSS), at 1,646,689 to 1,646,817
ydfB
b1573: ydfC - Qin prophage; predicted protein (RefSeq), at 1,646,847 to 1,647,065
ydfC
Position (kb)
1645
1646 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1644.647 kb on + strand, within flxA at 1644.647 kb on + strand, within flxA at 1644.656 kb on + strand, within flxA at 1644.690 kb on + strand, within flxA at 1644.756 kb on + strand at 1644.756 kb on + strand at 1644.758 kb on + strand at 1644.774 kb on + strand at 1644.787 kb on - strand at 1644.792 kb on - strand at 1644.809 kb on - strand at 1644.809 kb on - strand at 1644.870 kb on - strand at 1644.870 kb on - strand at 1644.870 kb on - strand at 1644.890 kb on - strand at 1644.918 kb on - strand at 1644.986 kb on - strand at 1645.019 kb on + strand at 1645.019 kb on + strand at 1645.027 kb on - strand at 1645.027 kb on - strand at 1645.038 kb on - strand at 1645.060 kb on + strand at 1645.062 kb on + strand at 1645.087 kb on + strand at 1645.116 kb on - strand at 1645.144 kb on + strand at 1645.145 kb on + strand at 1645.174 kb on - strand at 1645.176 kb on - strand at 1645.280 kb on + strand, within intK at 1645.288 kb on - strand, within intK at 1645.295 kb on + strand, within intK at 1645.408 kb on - strand at 1645.408 kb on - strand at 1645.546 kb on + strand, within ydfX at 1645.640 kb on + strand at 1645.640 kb on + strand at 1645.650 kb on - strand at 1645.725 kb on - strand, within dicC at 1645.725 kb on - strand, within dicC at 1645.940 kb on + strand at 1646.751 kb on - strand, within ydfB
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 1,644,647 + flxA b1566 0.65 +0.4 1,644,647 + flxA b1566 0.65 -0.1 1,644,656 + flxA b1566 0.68 -0.9 1,644,690 + flxA b1566 0.78 +0.0 1,644,756 + -0.3 1,644,756 + -0.9 1,644,758 + -1.2 1,644,774 + -1.8 1,644,787 - +0.7 1,644,792 - +1.7 1,644,809 - -0.6 1,644,809 - -1.9 1,644,870 - -0.3 1,644,870 - +0.7 1,644,870 - -1.9 1,644,890 - -0.6 1,644,918 - +0.0 1,644,986 - +0.6 1,645,019 + -0.9 1,645,019 + -0.9 1,645,027 - +0.2 1,645,027 - -0.6 1,645,038 - +0.1 1,645,060 + -0.1 1,645,062 + -0.6 1,645,087 + -1.6 1,645,116 - +0.9 1,645,144 + -0.9 1,645,145 + +3.1 1,645,174 - -0.5 1,645,176 - -0.6 1,645,280 + intK b1567 0.45 +0.5 1,645,288 - intK b1567 0.49 -1.0 1,645,295 + intK b1567 0.53 +3.0 1,645,408 - +0.1 1,645,408 - -0.4 1,645,546 + ydfX b1568 0.59 -0.5 1,645,640 + -0.3 1,645,640 + +0.7 1,645,650 - -2.5 1,645,725 - dicC b1569 0.35 +0.6 1,645,725 - dicC b1569 0.35 +1.4 1,645,940 + +0.1 1,646,751 - ydfB b1572 0.48 +0.7
Or see this region's nucleotide sequence