Strain Fitness in Escherichia coli BW25113 around b1540

Experiment: Cisplatin 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdcp and ydfG are separated by 136 nucleotidesydfG and ydfH are separated by 88 nucleotidesydfH and ydfZ are separated by 176 nucleotidesydfZ and ydfI are separated by 34 nucleotides b1538: dcp - dipeptidyl carboxypeptidase II (NCBI), at 1,623,359 to 1,625,404 dcp b1539: ydfG - L-allo-threonine dehydrogenase, NAD(P)-binding (NCBI), at 1,625,541 to 1,626,287 ydfG b1540: ydfH - predicted DNA-binding transcriptional regulator (NCBI), at 1,626,376 to 1,627,062 ydfH b1541: ydfZ - hypothetical protein (NCBI), at 1,627,239 to 1,627,442 ydfZ b1542: ydfI - predicted mannonate dehydrogenase (NCBI), at 1,627,477 to 1,628,937 ydfI Position (kb) 1626 1627 1628Strain fitness (log2 ratio) -2 -1 0 1at 1625.380 kb on + strandat 1625.442 kb on - strandat 1625.443 kb on - strandat 1625.443 kb on - strandat 1625.681 kb on + strand, within ydfGat 1625.871 kb on + strand, within ydfGat 1625.871 kb on + strand, within ydfGat 1626.043 kb on - strand, within ydfGat 1626.461 kb on + strand, within ydfHat 1626.955 kb on + strand, within ydfHat 1627.253 kb on - strandat 1627.424 kb on - strandat 1627.446 kb on + strandat 1627.454 kb on - strandat 1627.556 kb on - strandat 1627.675 kb on - strand, within ydfIat 1627.873 kb on + strand, within ydfI

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml
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1,625,380 + -0.7
1,625,442 - +1.1
1,625,443 - +1.2
1,625,443 - +1.4
1,625,681 + ydfG b1539 0.19 -0.0
1,625,871 + ydfG b1539 0.44 +0.3
1,625,871 + ydfG b1539 0.44 +0.4
1,626,043 - ydfG b1539 0.67 -0.3
1,626,461 + ydfH b1540 0.12 +1.6
1,626,955 + ydfH b1540 0.84 +1.1
1,627,253 - -1.5
1,627,424 - -0.0
1,627,446 + +0.3
1,627,454 - +0.8
1,627,556 - -0.2
1,627,675 - ydfI b1542 0.14 +0.5
1,627,873 + ydfI b1542 0.27 -1.8

Or see this region's nucleotide sequence