Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt citF and citE are separated by 10 nucleotides citE and citD overlap by 4 nucleotides citD and citC are separated by 14 nucleotides
b0615: citF - citrate lyase, citrate-ACP transferase (alpha) subunit (NCBI), at 647,262 to 648,794
citF
b0616: citE - citrate lyase beta chain (acyl lyase subunit) (VIMSS), at 648,805 to 649,713
citE
b0617: citD - citrate lyase, acyl carrier (gamma) subunit (NCBI), at 649,710 to 650,006
citD
b0618: citC - citrate lyase synthetase (citrate (pro-3S)-lyase ligase (VIMSS), at 650,021 to 651,079
citC
Position (kb) 648
649
650 Strain fitness (log2 ratio) -2
-1
0
1
2 at 648.197 kb on + strand, within citF at 648.197 kb on + strand, within citF at 648.551 kb on - strand, within citF at 648.551 kb on - strand, within citF at 648.610 kb on - strand, within citF at 648.645 kb on - strand at 648.676 kb on + strand at 648.676 kb on + strand at 648.810 kb on - strand at 649.122 kb on - strand, within citE at 649.238 kb on - strand, within citE at 649.238 kb on - strand, within citE at 649.327 kb on - strand, within citE at 649.479 kb on + strand, within citE at 649.540 kb on - strand, within citE at 649.569 kb on + strand, within citE at 649.595 kb on - strand, within citE at 649.595 kb on - strand, within citE at 649.607 kb on + strand, within citE at 649.642 kb on + strand at 649.665 kb on - strand at 649.828 kb on - strand, within citD at 649.828 kb on - strand, within citD at 649.831 kb on - strand, within citD at 649.947 kb on - strand, within citD at 650.199 kb on + strand, within citC at 650.221 kb on + strand, within citC at 650.310 kb on - strand, within citC at 650.326 kb on + strand, within citC at 650.332 kb on - strand, within citC at 650.332 kb on - strand at 650.456 kb on - strand, within citC at 650.577 kb on + strand, within citC
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 648,197 + citF b0615 0.61 -1.1 648,197 + citF b0615 0.61 +0.9 648,551 - citF b0615 0.84 -0.1 648,551 - citF b0615 0.84 -0.8 648,610 - citF b0615 0.88 +0.8 648,645 - +0.1 648,676 + -2.2 648,676 + -1.6 648,810 - +0.4 649,122 - citE b0616 0.35 -0.1 649,238 - citE b0616 0.48 -1.2 649,238 - citE b0616 0.48 +1.1 649,327 - citE b0616 0.57 +0.1 649,479 + citE b0616 0.74 -0.1 649,540 - citE b0616 0.81 -0.1 649,569 + citE b0616 0.84 +0.0 649,595 - citE b0616 0.87 +0.4 649,595 - citE b0616 0.87 +0.7 649,607 + citE b0616 0.88 +1.1 649,642 + -0.2 649,665 - -0.0 649,828 - citD b0617 0.40 +1.9 649,828 - citD b0617 0.40 -0.1 649,831 - citD b0617 0.41 +2.4 649,947 - citD b0617 0.80 -0.2 650,199 + citC b0618 0.17 +1.5 650,221 + citC b0618 0.19 -0.2 650,310 - citC b0618 0.27 +0.4 650,326 + citC b0618 0.29 -0.1 650,332 - citC b0618 0.29 +2.3 650,332 - -0.6 650,456 - citC b0618 0.41 +1.0 650,577 + citC b0618 0.53 +0.5
Or see this region's nucleotide sequence