Strain Fitness in Escherichia coli BW25113 around b3425

Experiment: Cisplatin 0.05 mg/ml

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntglpR and glpG are separated by 16 nucleotidesglpG and glpE are separated by 44 nucleotidesglpE and glpD are separated by 189 nucleotides b3423: glpR - DNA-binding transcriptional repressor (NCBI), at 3,557,870 to 3,558,628 glpR b3424: glpG - predicted intramembrane serine protease (NCBI), at 3,558,645 to 3,559,475 glpG b3425: glpE - thiosulfate sulfurtransferase (NCBI), at 3,559,520 to 3,559,846 glpE b3426: glpD - sn-glycerol-3-phosphate dehydrogenase, aerobic, FAD/NAD(P)-binding (NCBI), at 3,560,036 to 3,561,541 glpD Position (kb) 3559 3560Strain fitness (log2 ratio) -2 -1 0 1 2at 3558.523 kb on - strand, within glpRat 3558.572 kb on - strandat 3558.578 kb on + strandat 3558.578 kb on + strandat 3558.588 kb on - strandat 3558.606 kb on + strandat 3558.687 kb on + strandat 3558.753 kb on + strand, within glpGat 3558.766 kb on + strand, within glpGat 3558.774 kb on - strand, within glpGat 3558.776 kb on + strand, within glpGat 3558.776 kb on + strand, within glpGat 3558.791 kb on - strand, within glpGat 3558.794 kb on - strand, within glpGat 3558.816 kb on - strand, within glpGat 3558.851 kb on - strand, within glpGat 3558.855 kb on - strand, within glpGat 3558.867 kb on - strand, within glpGat 3558.867 kb on - strand, within glpGat 3558.919 kb on - strand, within glpGat 3558.921 kb on - strand, within glpGat 3558.921 kb on - strand, within glpGat 3558.927 kb on - strand, within glpGat 3558.975 kb on + strand, within glpGat 3558.991 kb on - strand, within glpGat 3559.003 kb on + strand, within glpGat 3559.041 kb on - strand, within glpGat 3559.041 kb on - strand, within glpGat 3559.049 kb on - strand, within glpGat 3559.056 kb on + strand, within glpGat 3559.056 kb on + strand, within glpGat 3559.058 kb on - strand, within glpGat 3559.058 kb on - strand, within glpGat 3559.117 kb on - strand, within glpGat 3559.127 kb on - strand, within glpGat 3559.127 kb on - strand, within glpGat 3559.129 kb on - strand, within glpGat 3559.160 kb on - strand, within glpGat 3559.180 kb on + strand, within glpGat 3559.180 kb on + strand, within glpGat 3559.185 kb on + strand, within glpGat 3559.193 kb on + strand, within glpGat 3559.223 kb on + strand, within glpGat 3559.223 kb on + strand, within glpGat 3559.234 kb on - strand, within glpGat 3559.259 kb on + strand, within glpGat 3559.262 kb on - strand, within glpGat 3559.262 kb on - strand, within glpGat 3559.325 kb on - strand, within glpGat 3559.342 kb on + strand, within glpGat 3559.350 kb on - strand, within glpGat 3559.350 kb on - strand, within glpGat 3559.350 kb on - strand, within glpGat 3559.350 kb on - strand, within glpGat 3559.370 kb on + strand, within glpGat 3559.370 kb on + strand, within glpGat 3559.383 kb on - strand, within glpGat 3559.405 kb on - strandat 3559.405 kb on - strandat 3559.419 kb on - strandat 3559.419 kb on - strandat 3559.439 kb on - strandat 3559.510 kb on + strandat 3559.510 kb on + strandat 3559.518 kb on - strandat 3559.583 kb on + strand, within glpEat 3559.584 kb on + strand, within glpEat 3559.587 kb on - strand, within glpEat 3559.592 kb on - strand, within glpEat 3559.592 kb on - strand, within glpEat 3559.603 kb on - strand, within glpEat 3559.624 kb on - strand, within glpEat 3559.661 kb on + strand, within glpEat 3559.663 kb on + strand, within glpEat 3559.663 kb on + strand, within glpEat 3559.715 kb on + strand, within glpEat 3559.719 kb on + strand, within glpEat 3559.719 kb on + strand, within glpEat 3559.735 kb on - strand, within glpEat 3559.737 kb on + strand, within glpEat 3559.741 kb on + strand, within glpEat 3559.745 kb on - strand, within glpEat 3559.822 kb on + strandat 3559.848 kb on - strandat 3559.891 kb on + strandat 3559.899 kb on - strandat 3559.922 kb on + strandat 3559.927 kb on + strandat 3559.927 kb on + strandat 3559.930 kb on - strandat 3560.077 kb on - strandat 3560.194 kb on + strand, within glpDat 3560.199 kb on + strand, within glpDat 3560.265 kb on - strand, within glpDat 3560.273 kb on + strand, within glpDat 3560.358 kb on - strand, within glpDat 3560.358 kb on - strand, within glpDat 3560.369 kb on + strand, within glpDat 3560.466 kb on + strand, within glpDat 3560.594 kb on + strand, within glpDat 3560.657 kb on - strand, within glpDat 3560.657 kb on - strand, within glpDat 3560.730 kb on + strand, within glpDat 3560.738 kb on - strand, within glpDat 3560.767 kb on + strand, within glpDat 3560.775 kb on - strand, within glpDat 3560.803 kb on + strand, within glpDat 3560.803 kb on + strand, within glpD

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml
remove
3,558,523 - glpR b3423 0.86 -0.5
3,558,572 - -0.8
3,558,578 + -0.3
3,558,578 + +0.2
3,558,588 - +0.2
3,558,606 + +0.1
3,558,687 + +1.4
3,558,753 + glpG b3424 0.13 -0.5
3,558,766 + glpG b3424 0.15 -0.7
3,558,774 - glpG b3424 0.16 -0.4
3,558,776 + glpG b3424 0.16 -1.2
3,558,776 + glpG b3424 0.16 +0.5
3,558,791 - glpG b3424 0.18 -0.2
3,558,794 - glpG b3424 0.18 +0.2
3,558,816 - glpG b3424 0.21 -0.4
3,558,851 - glpG b3424 0.25 +0.4
3,558,855 - glpG b3424 0.25 -1.0
3,558,867 - glpG b3424 0.27 -0.5
3,558,867 - glpG b3424 0.27 +0.9
3,558,919 - glpG b3424 0.33 -0.2
3,558,921 - glpG b3424 0.33 -0.4
3,558,921 - glpG b3424 0.33 -0.3
3,558,927 - glpG b3424 0.34 +0.3
3,558,975 + glpG b3424 0.40 +0.6
3,558,991 - glpG b3424 0.42 -0.1
3,559,003 + glpG b3424 0.43 +0.1
3,559,041 - glpG b3424 0.48 -1.3
3,559,041 - glpG b3424 0.48 -0.4
3,559,049 - glpG b3424 0.49 -0.2
3,559,056 + glpG b3424 0.49 -0.8
3,559,056 + glpG b3424 0.49 -0.1
3,559,058 - glpG b3424 0.50 -0.9
3,559,058 - glpG b3424 0.50 -0.2
3,559,117 - glpG b3424 0.57 +0.0
3,559,127 - glpG b3424 0.58 +0.6
3,559,127 - glpG b3424 0.58 +1.0
3,559,129 - glpG b3424 0.58 -1.0
3,559,160 - glpG b3424 0.62 -1.0
3,559,180 + glpG b3424 0.64 -0.9
3,559,180 + glpG b3424 0.64 +1.0
3,559,185 + glpG b3424 0.65 -0.9
3,559,193 + glpG b3424 0.66 -0.7
3,559,223 + glpG b3424 0.70 -0.6
3,559,223 + glpG b3424 0.70 -0.5
3,559,234 - glpG b3424 0.71 +0.3
3,559,259 + glpG b3424 0.74 -0.3
3,559,262 - glpG b3424 0.74 -0.2
3,559,262 - glpG b3424 0.74 -0.4
3,559,325 - glpG b3424 0.82 -0.4
3,559,342 + glpG b3424 0.84 +0.1
3,559,350 - glpG b3424 0.85 +0.0
3,559,350 - glpG b3424 0.85 -0.1
3,559,350 - glpG b3424 0.85 -0.3
3,559,350 - glpG b3424 0.85 +0.8
3,559,370 + glpG b3424 0.87 +0.7
3,559,370 + glpG b3424 0.87 -0.3
3,559,383 - glpG b3424 0.89 +0.9
3,559,405 - -0.9
3,559,405 - +0.1
3,559,419 - +0.3
3,559,419 - +1.1
3,559,439 - -0.3
3,559,510 + -0.3
3,559,510 + -0.6
3,559,518 - +0.6
3,559,583 + glpE b3425 0.19 +0.4
3,559,584 + glpE b3425 0.20 +0.3
3,559,587 - glpE b3425 0.20 +0.1
3,559,592 - glpE b3425 0.22 +0.0
3,559,592 - glpE b3425 0.22 -1.9
3,559,603 - glpE b3425 0.25 +0.9
3,559,624 - glpE b3425 0.32 +0.3
3,559,661 + glpE b3425 0.43 +1.0
3,559,663 + glpE b3425 0.44 +0.7
3,559,663 + glpE b3425 0.44 +0.7
3,559,715 + glpE b3425 0.60 -0.1
3,559,719 + glpE b3425 0.61 -0.1
3,559,719 + glpE b3425 0.61 +0.3
3,559,735 - glpE b3425 0.66 +0.8
3,559,737 + glpE b3425 0.66 +0.0
3,559,741 + glpE b3425 0.68 -2.0
3,559,745 - glpE b3425 0.69 +2.0
3,559,822 + +1.3
3,559,848 - -0.5
3,559,891 + -0.2
3,559,899 - -0.5
3,559,922 + +0.1
3,559,927 + -0.3
3,559,927 + +0.3
3,559,930 - -0.1
3,560,077 - -1.3
3,560,194 + glpD b3426 0.10 +1.0
3,560,199 + glpD b3426 0.11 +0.7
3,560,265 - glpD b3426 0.15 +1.1
3,560,273 + glpD b3426 0.16 +0.7
3,560,358 - glpD b3426 0.21 -0.3
3,560,358 - glpD b3426 0.21 -0.9
3,560,369 + glpD b3426 0.22 -0.6
3,560,466 + glpD b3426 0.29 +1.3
3,560,594 + glpD b3426 0.37 +0.1
3,560,657 - glpD b3426 0.41 +0.1
3,560,657 - glpD b3426 0.41 +0.7
3,560,730 + glpD b3426 0.46 +0.7
3,560,738 - glpD b3426 0.47 +0.7
3,560,767 + glpD b3426 0.49 -0.7
3,560,775 - glpD b3426 0.49 -2.3
3,560,803 + glpD b3426 0.51 -0.3
3,560,803 + glpD b3426 0.51 +0.7

Or see this region's nucleotide sequence