Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yecC and yecS overlap by 4 nucleotides yecS and yedO are separated by 14 nucleotides yedO and fliY are separated by 104 nucleotides fliY and fliZ are separated by 87 nucleotides
b1917: yecC - predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 1,995,086 to 1,995,838
yecC
b1918: yecS - predicted transporter subunit: membrane component of ABC superfamily (NCBI), at 1,995,835 to 1,996,503
yecS
b1919: yedO - putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS), at 1,996,518 to 1,997,504
yedO
b1920: fliY - cystine transporter subunit (NCBI), at 1,997,609 to 1,998,409
fliY
b1921: fliZ - orf, hypothetical protein (VIMSS), at 1,998,497 to 1,999,048
fliZ
Position (kb)
1996
1997
1998 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1995.592 kb on + strand, within yecC at 1995.598 kb on - strand, within yecC at 1995.598 kb on - strand, within yecC at 1995.688 kb on + strand, within yecC at 1995.771 kb on - strand at 1995.777 kb on - strand at 1995.777 kb on - strand at 1995.779 kb on + strand at 1995.779 kb on + strand at 1995.779 kb on + strand at 1995.779 kb on + strand at 1995.880 kb on + strand at 1995.880 kb on + strand at 1995.968 kb on + strand, within yecS at 1995.976 kb on - strand, within yecS at 1996.355 kb on + strand, within yecS at 1996.445 kb on - strand at 1996.445 kb on - strand at 1996.446 kb on - strand at 1996.778 kb on - strand, within yedO at 1997.064 kb on - strand, within yedO at 1997.231 kb on - strand, within yedO at 1997.231 kb on - strand, within yedO at 1997.256 kb on - strand, within yedO at 1997.658 kb on + strand at 1997.811 kb on + strand, within fliY at 1997.811 kb on + strand, within fliY at 1997.931 kb on - strand, within fliY at 1998.266 kb on + strand, within fliY at 1998.375 kb on + strand at 1998.375 kb on + strand at 1998.387 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 1,995,592 + yecC b1917 0.67 +0.0 1,995,598 - yecC b1917 0.68 +1.9 1,995,598 - yecC b1917 0.68 +0.2 1,995,688 + yecC b1917 0.80 +0.0 1,995,771 - -0.3 1,995,777 - +1.1 1,995,777 - -0.0 1,995,779 + +0.3 1,995,779 + +0.4 1,995,779 + +0.3 1,995,779 + -0.8 1,995,880 + +0.2 1,995,880 + +1.0 1,995,968 + yecS b1918 0.20 +0.2 1,995,976 - yecS b1918 0.21 -0.2 1,996,355 + yecS b1918 0.78 +0.0 1,996,445 - +0.6 1,996,445 - +0.1 1,996,446 - +1.6 1,996,778 - yedO b1919 0.26 +0.8 1,997,064 - yedO b1919 0.55 +0.4 1,997,231 - yedO b1919 0.72 +1.0 1,997,231 - yedO b1919 0.72 +0.7 1,997,256 - yedO b1919 0.75 +0.2 1,997,658 + -0.0 1,997,811 + fliY b1920 0.25 +0.3 1,997,811 + fliY b1920 0.25 -0.7 1,997,931 - fliY b1920 0.40 -0.7 1,998,266 + fliY b1920 0.82 +0.1 1,998,375 + -2.3 1,998,375 + -0.8 1,998,387 - +0.2
Or see this region's nucleotide sequence