Strain Fitness in Escherichia coli BW25113 around b1114

Experiment: Cisplatin 0.05 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycfS and mfd are separated by 143 nucleotidesmfd and ycfT are separated by 127 nucleotides b1113: ycfS - hypothetical protein (NCBI), at 1,168,635 to 1,169,597 ycfS b1114: mfd - transcription-repair coupling factor (NCBI), at 1,169,741 to 1,173,187 mfd b1115: ycfT - predicted inner membrane protein (NCBI), at 1,173,315 to 1,174,388 ycfT Position (kb) 1169 1170 1171 1172 1173 1174Strain fitness (log2 ratio) -2 -1 0 1 2at 1168.746 kb on + strand, within ycfSat 1168.766 kb on + strand, within ycfSat 1168.802 kb on + strand, within ycfSat 1168.805 kb on + strand, within ycfSat 1168.870 kb on + strand, within ycfSat 1168.925 kb on - strand, within ycfSat 1169.013 kb on + strand, within ycfSat 1169.180 kb on + strand, within ycfSat 1169.204 kb on + strand, within ycfSat 1169.204 kb on + strand, within ycfSat 1169.259 kb on - strand, within ycfSat 1169.270 kb on + strand, within ycfSat 1169.654 kb on - strandat 1169.664 kb on + strandat 1169.664 kb on + strandat 1169.666 kb on + strandat 1169.674 kb on + strandat 1169.674 kb on + strandat 1169.682 kb on - strandat 1170.000 kb on + strandat 1170.000 kb on + strandat 1170.148 kb on - strand, within mfdat 1170.304 kb on - strand, within mfdat 1170.667 kb on + strand, within mfdat 1170.747 kb on + strand, within mfdat 1170.747 kb on + strand, within mfdat 1170.806 kb on + strand, within mfdat 1170.880 kb on - strand, within mfdat 1170.970 kb on + strand, within mfdat 1170.970 kb on + strand, within mfdat 1171.193 kb on + strand, within mfdat 1171.218 kb on + strand, within mfdat 1171.228 kb on - strand, within mfdat 1171.228 kb on - strand, within mfdat 1171.252 kb on - strand, within mfdat 1171.252 kb on - strand, within mfdat 1171.294 kb on + strand, within mfdat 1171.294 kb on + strand, within mfdat 1171.403 kb on + strand, within mfdat 1171.451 kb on + strand, within mfdat 1171.655 kb on + strand, within mfdat 1171.939 kb on + strand, within mfdat 1172.040 kb on - strand, within mfdat 1172.040 kb on - strand, within mfdat 1172.210 kb on - strand, within mfdat 1172.574 kb on - strand, within mfdat 1172.758 kb on - strand, within mfdat 1172.772 kb on + strand, within mfdat 1172.872 kb on + strandat 1172.925 kb on + strandat 1172.941 kb on + strandat 1173.058 kb on + strandat 1173.058 kb on + strandat 1173.058 kb on + strandat 1173.058 kb on + strandat 1173.071 kb on + strandat 1173.089 kb on + strandat 1173.148 kb on - strandat 1173.173 kb on - strandat 1173.192 kb on + strandat 1173.218 kb on - strandat 1173.463 kb on - strand, within ycfTat 1173.506 kb on - strand, within ycfTat 1173.506 kb on - strand, within ycfTat 1173.541 kb on - strand, within ycfTat 1173.593 kb on + strand, within ycfTat 1173.602 kb on - strand, within ycfTat 1173.602 kb on - strand, within ycfTat 1173.710 kb on + strand, within ycfTat 1173.720 kb on + strand, within ycfTat 1173.720 kb on + strand, within ycfTat 1173.747 kb on + strand, within ycfTat 1173.757 kb on - strand, within ycfTat 1173.757 kb on - strand, within ycfTat 1173.767 kb on - strand, within ycfTat 1173.791 kb on + strand, within ycfTat 1173.791 kb on + strand, within ycfTat 1174.091 kb on - strand, within ycfTat 1174.091 kb on - strand, within ycfTat 1174.111 kb on + strand, within ycfTat 1174.176 kb on - strand, within ycfTat 1174.176 kb on - strand, within ycfT

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml
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1,168,746 + ycfS b1113 0.12 +0.1
1,168,766 + ycfS b1113 0.14 +2.3
1,168,802 + ycfS b1113 0.17 +0.0
1,168,805 + ycfS b1113 0.18 -0.2
1,168,870 + ycfS b1113 0.24 -0.6
1,168,925 - ycfS b1113 0.30 +0.0
1,169,013 + ycfS b1113 0.39 +0.7
1,169,180 + ycfS b1113 0.57 -0.0
1,169,204 + ycfS b1113 0.59 -0.1
1,169,204 + ycfS b1113 0.59 +1.2
1,169,259 - ycfS b1113 0.65 +1.1
1,169,270 + ycfS b1113 0.66 -0.5
1,169,654 - +0.5
1,169,664 + +0.0
1,169,664 + -0.0
1,169,666 + +0.2
1,169,674 + +0.1
1,169,674 + +0.2
1,169,682 - +0.1
1,170,000 + -1.2
1,170,000 + -0.3
1,170,148 - mfd b1114 0.12 -0.1
1,170,304 - mfd b1114 0.16 -1.9
1,170,667 + mfd b1114 0.27 -1.0
1,170,747 + mfd b1114 0.29 -0.1
1,170,747 + mfd b1114 0.29 -1.5
1,170,806 + mfd b1114 0.31 -0.5
1,170,880 - mfd b1114 0.33 -2.7
1,170,970 + mfd b1114 0.36 -2.0
1,170,970 + mfd b1114 0.36 +0.2
1,171,193 + mfd b1114 0.42 -0.5
1,171,218 + mfd b1114 0.43 -1.1
1,171,228 - mfd b1114 0.43 -1.4
1,171,228 - mfd b1114 0.43 -0.8
1,171,252 - mfd b1114 0.44 -0.8
1,171,252 - mfd b1114 0.44 +0.7
1,171,294 + mfd b1114 0.45 -1.8
1,171,294 + mfd b1114 0.45 +1.5
1,171,403 + mfd b1114 0.48 -1.7
1,171,451 + mfd b1114 0.50 -0.8
1,171,655 + mfd b1114 0.56 -1.1
1,171,939 + mfd b1114 0.64 -0.7
1,172,040 - mfd b1114 0.67 +0.2
1,172,040 - mfd b1114 0.67 -0.2
1,172,210 - mfd b1114 0.72 -1.7
1,172,574 - mfd b1114 0.82 -1.8
1,172,758 - mfd b1114 0.88 -1.7
1,172,772 + mfd b1114 0.88 -2.0
1,172,872 + -2.0
1,172,925 + -0.9
1,172,941 + -0.8
1,173,058 + -1.5
1,173,058 + -1.8
1,173,058 + -1.0
1,173,058 + -1.9
1,173,071 + -2.2
1,173,089 + -1.8
1,173,148 - -0.8
1,173,173 - +0.8
1,173,192 + -0.1
1,173,218 - -0.4
1,173,463 - ycfT b1115 0.14 -0.3
1,173,506 - ycfT b1115 0.18 +0.2
1,173,506 - ycfT b1115 0.18 +0.6
1,173,541 - ycfT b1115 0.21 +0.4
1,173,593 + ycfT b1115 0.26 -0.7
1,173,602 - ycfT b1115 0.27 -0.7
1,173,602 - ycfT b1115 0.27 -0.2
1,173,710 + ycfT b1115 0.37 -2.0
1,173,720 + ycfT b1115 0.38 -0.3
1,173,720 + ycfT b1115 0.38 -0.9
1,173,747 + ycfT b1115 0.40 +0.5
1,173,757 - ycfT b1115 0.41 -0.1
1,173,757 - ycfT b1115 0.41 +0.5
1,173,767 - ycfT b1115 0.42 +0.3
1,173,791 + ycfT b1115 0.44 +1.5
1,173,791 + ycfT b1115 0.44 +0.1
1,174,091 - ycfT b1115 0.72 +0.3
1,174,091 - ycfT b1115 0.72 +0.1
1,174,111 + ycfT b1115 0.74 -0.3
1,174,176 - ycfT b1115 0.80 -0.2
1,174,176 - ycfT b1115 0.80 -0.4

Or see this region's nucleotide sequence