Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt proC and yaiI are separated by 119 nucleotides yaiI and aroL are separated by 182 nucleotides aroL and yaiA are separated by 49 nucleotides yaiA and aroM are separated by 257 nucleotides
b0386: proC - pyrroline-5-carboxylate reductase (NCBI), at 404,059 to 404,868
proC
b0387: yaiI - orf, hypothetical protein (VIMSS), at 404,988 to 405,446
yaiI
b0388: aroL - shikimate kinase II (NCBI), at 405,629 to 406,153
aroL
b0389: yaiA - hypothetical protein (NCBI), at 406,203 to 406,394
yaiA
b0390: aroM - hypothetical protein (NCBI), at 406,652 to 407,329
aroM
Position (kb) 405
406
407 Strain fitness (log2 ratio) -1
0
1
2
3 at 404.717 kb on + strand, within proC at 404.717 kb on + strand, within proC at 404.778 kb on + strand, within proC at 405.063 kb on - strand, within yaiI at 405.237 kb on + strand, within yaiI at 405.352 kb on - strand, within yaiI at 405.370 kb on - strand, within yaiI at 405.370 kb on - strand, within yaiI at 405.370 kb on - strand, within yaiI at 405.584 kb on - strand at 405.584 kb on - strand at 405.675 kb on - strand at 405.867 kb on - strand, within aroL at 405.944 kb on + strand, within aroL at 405.944 kb on + strand, within aroL at 405.944 kb on + strand, within aroL at 405.952 kb on - strand, within aroL at 405.952 kb on - strand, within aroL at 406.049 kb on + strand, within aroL at 406.057 kb on - strand, within aroL at 406.095 kb on + strand, within aroL at 406.169 kb on + strand at 406.225 kb on - strand, within yaiA at 406.381 kb on + strand at 406.391 kb on - strand at 406.391 kb on - strand at 406.405 kb on - strand at 406.407 kb on + strand at 406.592 kb on + strand at 406.597 kb on + strand at 406.683 kb on + strand at 406.688 kb on + strand at 406.688 kb on + strand at 406.688 kb on + strand at 406.688 kb on + strand at 406.691 kb on - strand at 406.749 kb on + strand, within aroM at 406.757 kb on + strand, within aroM at 406.757 kb on + strand, within aroM at 406.776 kb on + strand, within aroM at 406.807 kb on + strand, within aroM at 406.819 kb on - strand, within aroM at 406.819 kb on - strand, within aroM at 406.913 kb on + strand, within aroM at 406.913 kb on + strand, within aroM at 406.913 kb on + strand, within aroM at 406.942 kb on + strand, within aroM at 406.942 kb on + strand, within aroM at 406.960 kb on + strand, within aroM at 406.960 kb on - strand, within aroM at 406.960 kb on - strand, within aroM at 406.994 kb on - strand, within aroM at 407.020 kb on + strand, within aroM at 407.052 kb on - strand, within aroM at 407.052 kb on - strand, within aroM at 407.131 kb on + strand, within aroM at 407.139 kb on - strand, within aroM at 407.139 kb on - strand, within aroM at 407.139 kb on - strand, within aroM at 407.139 kb on - strand, within aroM
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 404,717 + proC b0386 0.81 -0.6 404,717 + proC b0386 0.81 -1.3 404,778 + proC b0386 0.89 +0.4 405,063 - yaiI b0387 0.16 -0.3 405,237 + yaiI b0387 0.54 -0.1 405,352 - yaiI b0387 0.79 -0.5 405,370 - yaiI b0387 0.83 +0.6 405,370 - yaiI b0387 0.83 -0.1 405,370 - yaiI b0387 0.83 +0.5 405,584 - +2.0 405,584 - +0.0 405,675 - +0.2 405,867 - aroL b0388 0.45 -1.7 405,944 + aroL b0388 0.60 +1.9 405,944 + aroL b0388 0.60 +1.9 405,944 + aroL b0388 0.60 +1.6 405,952 - aroL b0388 0.62 +0.5 405,952 - aroL b0388 0.62 +0.0 406,049 + aroL b0388 0.80 +1.8 406,057 - aroL b0388 0.82 +1.7 406,095 + aroL b0388 0.89 +2.5 406,169 + +0.2 406,225 - yaiA b0389 0.11 +0.4 406,381 + +1.3 406,391 - +0.2 406,391 - -0.3 406,405 - -0.0 406,407 + +3.0 406,592 + +3.2 406,597 + +2.8 406,683 + -0.5 406,688 + -0.1 406,688 + -0.8 406,688 + -0.4 406,688 + -0.8 406,691 - -0.8 406,749 + aroM b0390 0.14 +0.2 406,757 + aroM b0390 0.15 -1.1 406,757 + aroM b0390 0.15 -0.2 406,776 + aroM b0390 0.18 -0.8 406,807 + aroM b0390 0.23 -1.7 406,819 - aroM b0390 0.25 +1.0 406,819 - aroM b0390 0.25 -1.3 406,913 + aroM b0390 0.38 -0.1 406,913 + aroM b0390 0.38 -0.9 406,913 + aroM b0390 0.38 -0.5 406,942 + aroM b0390 0.43 +0.5 406,942 + aroM b0390 0.43 +0.1 406,960 + aroM b0390 0.45 -0.4 406,960 - aroM b0390 0.45 -1.6 406,960 - aroM b0390 0.45 +0.0 406,994 - aroM b0390 0.50 -0.2 407,020 + aroM b0390 0.54 +0.0 407,052 - aroM b0390 0.59 +0.3 407,052 - aroM b0390 0.59 +0.4 407,131 + aroM b0390 0.71 -0.7 407,139 - aroM b0390 0.72 -0.7 407,139 - aroM b0390 0.72 -0.7 407,139 - aroM b0390 0.72 +0.4 407,139 - aroM b0390 0.72 +0.1
Or see this region's nucleotide sequence