Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt folA and apaH are separated by 77 nucleotides apaH and apaG are separated by 6 nucleotides apaG and ksgA are separated by 2 nucleotides ksgA and pdxA overlap by 4 nucleotides
b0048: folA - dihydrofolate reductase (NCBI), at 49,823 to 50,302
folA
b0049: apaH - diadenosinetetraphosphatase (NCBI), at 50,380 to 51,222
apaH
b0050: apaG - hypothetical protein (NCBI), at 51,229 to 51,606
apaG
b0051: ksgA - dimethyladenosine transferase (NCBI), at 51,609 to 52,430
ksgA
b0052: pdxA - 4-hydroxythreonine-4-phosphate dehydrogenase (NCBI), at 52,427 to 53,416
pdxA
Position (kb)
51
52 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 50.361 kb on + strand at 50.361 kb on + strand at 50.370 kb on - strand at 50.376 kb on + strand at 50.461 kb on + strand at 50.469 kb on - strand, within apaH at 50.501 kb on + strand, within apaH at 50.507 kb on - strand, within apaH at 50.568 kb on - strand, within apaH at 50.717 kb on - strand, within apaH at 50.785 kb on - strand, within apaH at 50.791 kb on + strand, within apaH at 50.802 kb on + strand, within apaH at 50.829 kb on - strand, within apaH at 50.859 kb on - strand, within apaH at 50.877 kb on + strand, within apaH at 50.877 kb on + strand, within apaH at 51.025 kb on + strand, within apaH at 51.109 kb on - strand, within apaH at 51.177 kb on + strand at 51.177 kb on + strand at 51.185 kb on - strand at 51.190 kb on + strand at 51.190 kb on + strand at 51.240 kb on + strand at 51.240 kb on + strand at 51.311 kb on + strand, within apaG at 51.311 kb on + strand, within apaG at 51.322 kb on - strand, within apaG at 51.326 kb on - strand, within apaG at 51.334 kb on - strand, within apaG at 51.336 kb on - strand, within apaG at 51.353 kb on + strand, within apaG at 51.353 kb on + strand, within apaG at 51.353 kb on - strand, within apaG at 51.364 kb on - strand, within apaG at 51.372 kb on - strand, within apaG at 51.415 kb on + strand, within apaG at 51.423 kb on - strand, within apaG at 51.466 kb on + strand, within apaG at 51.472 kb on + strand, within apaG at 51.512 kb on - strand, within apaG at 51.514 kb on - strand, within apaG at 51.520 kb on - strand, within apaG at 51.524 kb on + strand, within apaG at 51.550 kb on - strand, within apaG at 51.560 kb on + strand, within apaG at 51.567 kb on - strand, within apaG at 51.567 kb on - strand, within apaG at 51.576 kb on + strand at 51.576 kb on + strand at 51.586 kb on - strand at 51.613 kb on + strand at 51.614 kb on - strand at 51.615 kb on + strand at 51.615 kb on + strand at 51.673 kb on - strand at 51.703 kb on + strand, within ksgA at 51.703 kb on + strand, within ksgA at 51.812 kb on - strand, within ksgA at 51.836 kb on + strand, within ksgA at 51.891 kb on - strand, within ksgA at 51.891 kb on - strand, within ksgA at 51.904 kb on + strand, within ksgA at 51.906 kb on + strand, within ksgA at 51.925 kb on - strand, within ksgA at 52.040 kb on + strand, within ksgA at 52.040 kb on + strand, within ksgA at 52.040 kb on + strand, within ksgA at 52.042 kb on + strand, within ksgA at 52.042 kb on + strand, within ksgA at 52.042 kb on + strand, within ksgA at 52.042 kb on - strand, within ksgA at 52.042 kb on - strand, within ksgA at 52.045 kb on + strand, within ksgA at 52.062 kb on + strand, within ksgA at 52.062 kb on + strand at 52.070 kb on - strand, within ksgA at 52.072 kb on + strand, within ksgA at 52.089 kb on - strand, within ksgA at 52.276 kb on + strand, within ksgA at 52.276 kb on + strand, within ksgA at 52.284 kb on + strand, within ksgA at 52.338 kb on + strand, within ksgA at 52.338 kb on + strand, within ksgA at 52.343 kb on - strand, within ksgA at 52.348 kb on - strand at 52.348 kb on - strand at 52.348 kb on - strand at 52.354 kb on + strand at 52.420 kb on - strand at 52.490 kb on + strand at 52.490 kb on + strand at 52.502 kb on + strand at 52.502 kb on + strand at 52.518 kb on - strand at 52.518 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 50,361 + +0.2 50,361 + -0.0 50,370 - -0.6 50,376 + -0.3 50,461 + -1.2 50,469 - apaH b0049 0.11 -1.8 50,501 + apaH b0049 0.14 +0.5 50,507 - apaH b0049 0.15 +0.6 50,568 - apaH b0049 0.22 -1.2 50,717 - apaH b0049 0.40 -2.3 50,785 - apaH b0049 0.48 -0.5 50,791 + apaH b0049 0.49 +2.1 50,802 + apaH b0049 0.50 -0.2 50,829 - apaH b0049 0.53 +2.1 50,859 - apaH b0049 0.57 -1.4 50,877 + apaH b0049 0.59 +2.1 50,877 + apaH b0049 0.59 +2.1 51,025 + apaH b0049 0.77 -2.0 51,109 - apaH b0049 0.86 -2.4 51,177 + +0.5 51,177 + -1.2 51,185 - -2.2 51,190 + -1.8 51,190 + -2.1 51,240 + +0.9 51,240 + +1.6 51,311 + apaG b0050 0.22 +0.4 51,311 + apaG b0050 0.22 +1.1 51,322 - apaG b0050 0.25 +1.1 51,326 - apaG b0050 0.26 +1.1 51,334 - apaG b0050 0.28 +2.2 51,336 - apaG b0050 0.28 +0.9 51,353 + apaG b0050 0.33 +1.6 51,353 + apaG b0050 0.33 +0.7 51,353 - apaG b0050 0.33 +1.0 51,364 - apaG b0050 0.36 +0.8 51,372 - apaG b0050 0.38 +1.1 51,415 + apaG b0050 0.49 +1.2 51,423 - apaG b0050 0.51 +0.7 51,466 + apaG b0050 0.63 -0.2 51,472 + apaG b0050 0.64 +0.7 51,512 - apaG b0050 0.75 +0.9 51,514 - apaG b0050 0.75 +1.3 51,520 - apaG b0050 0.77 +0.1 51,524 + apaG b0050 0.78 +0.9 51,550 - apaG b0050 0.85 +1.0 51,560 + apaG b0050 0.88 +0.6 51,567 - apaG b0050 0.89 +1.4 51,567 - apaG b0050 0.89 +1.0 51,576 + +0.4 51,576 + +0.9 51,586 - +0.8 51,613 + +0.2 51,614 - -0.7 51,615 + -1.6 51,615 + -0.1 51,673 - -1.1 51,703 + ksgA b0051 0.11 +1.7 51,703 + ksgA b0051 0.11 +0.1 51,812 - ksgA b0051 0.25 +0.5 51,836 + ksgA b0051 0.28 +3.1 51,891 - ksgA b0051 0.34 +1.7 51,891 - ksgA b0051 0.34 -0.6 51,904 + ksgA b0051 0.36 +2.1 51,906 + ksgA b0051 0.36 -0.5 51,925 - ksgA b0051 0.38 -0.4 52,040 + ksgA b0051 0.52 +0.6 52,040 + ksgA b0051 0.52 -0.4 52,040 + ksgA b0051 0.52 +0.8 52,042 + ksgA b0051 0.53 -0.3 52,042 + ksgA b0051 0.53 +1.3 52,042 + ksgA b0051 0.53 -1.2 52,042 - ksgA b0051 0.53 -0.5 52,042 - ksgA b0051 0.53 +0.5 52,045 + ksgA b0051 0.53 -0.7 52,062 + ksgA b0051 0.55 +1.5 52,062 + +1.3 52,070 - ksgA b0051 0.56 -0.9 52,072 + ksgA b0051 0.56 +4.1 52,089 - ksgA b0051 0.58 -0.2 52,276 + ksgA b0051 0.81 -2.2 52,276 + ksgA b0051 0.81 -0.4 52,284 + ksgA b0051 0.82 -0.3 52,338 + ksgA b0051 0.89 -0.5 52,338 + ksgA b0051 0.89 +1.1 52,343 - ksgA b0051 0.89 +0.4 52,348 - -0.9 52,348 - -0.1 52,348 - +0.8 52,354 + +1.4 52,420 - -0.7 52,490 + +0.7 52,490 + +0.2 52,502 + -0.6 52,502 + +0.3 52,518 - +0.3 52,518 - -1.0
Or see this region's nucleotide sequence