Strain Fitness in Escherichia coli BW25113 around b0050

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfolA and apaH are separated by 77 nucleotidesapaH and apaG are separated by 6 nucleotidesapaG and ksgA are separated by 2 nucleotidesksgA and pdxA overlap by 4 nucleotides b0048: folA - dihydrofolate reductase (NCBI), at 49,823 to 50,302 folA b0049: apaH - diadenosinetetraphosphatase (NCBI), at 50,380 to 51,222 apaH b0050: apaG - hypothetical protein (NCBI), at 51,229 to 51,606 apaG b0051: ksgA - dimethyladenosine transferase (NCBI), at 51,609 to 52,430 ksgA b0052: pdxA - 4-hydroxythreonine-4-phosphate dehydrogenase (NCBI), at 52,427 to 53,416 pdxA Position (kb) 51 52Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 50.361 kb on + strandat 50.361 kb on + strandat 50.370 kb on - strandat 50.376 kb on + strandat 50.461 kb on + strandat 50.469 kb on - strand, within apaHat 50.501 kb on + strand, within apaHat 50.507 kb on - strand, within apaHat 50.568 kb on - strand, within apaHat 50.717 kb on - strand, within apaHat 50.785 kb on - strand, within apaHat 50.791 kb on + strand, within apaHat 50.802 kb on + strand, within apaHat 50.829 kb on - strand, within apaHat 50.859 kb on - strand, within apaHat 50.877 kb on + strand, within apaHat 50.877 kb on + strand, within apaHat 51.025 kb on + strand, within apaHat 51.109 kb on - strand, within apaHat 51.177 kb on + strandat 51.177 kb on + strandat 51.185 kb on - strandat 51.190 kb on + strandat 51.190 kb on + strandat 51.240 kb on + strandat 51.240 kb on + strandat 51.311 kb on + strand, within apaGat 51.311 kb on + strand, within apaGat 51.322 kb on - strand, within apaGat 51.326 kb on - strand, within apaGat 51.334 kb on - strand, within apaGat 51.336 kb on - strand, within apaGat 51.353 kb on + strand, within apaGat 51.353 kb on + strand, within apaGat 51.353 kb on - strand, within apaGat 51.364 kb on - strand, within apaGat 51.372 kb on - strand, within apaGat 51.415 kb on + strand, within apaGat 51.423 kb on - strand, within apaGat 51.466 kb on + strand, within apaGat 51.472 kb on + strand, within apaGat 51.512 kb on - strand, within apaGat 51.514 kb on - strand, within apaGat 51.520 kb on - strand, within apaGat 51.524 kb on + strand, within apaGat 51.550 kb on - strand, within apaGat 51.560 kb on + strand, within apaGat 51.567 kb on - strand, within apaGat 51.567 kb on - strand, within apaGat 51.576 kb on + strandat 51.576 kb on + strandat 51.586 kb on - strandat 51.613 kb on + strandat 51.614 kb on - strandat 51.615 kb on + strandat 51.615 kb on + strandat 51.673 kb on - strandat 51.703 kb on + strand, within ksgAat 51.703 kb on + strand, within ksgAat 51.812 kb on - strand, within ksgAat 51.836 kb on + strand, within ksgAat 51.891 kb on - strand, within ksgAat 51.891 kb on - strand, within ksgAat 51.904 kb on + strand, within ksgAat 51.906 kb on + strand, within ksgAat 51.925 kb on - strand, within ksgAat 52.040 kb on + strand, within ksgAat 52.040 kb on + strand, within ksgAat 52.040 kb on + strand, within ksgAat 52.042 kb on + strand, within ksgAat 52.042 kb on + strand, within ksgAat 52.042 kb on + strand, within ksgAat 52.042 kb on - strand, within ksgAat 52.042 kb on - strand, within ksgAat 52.045 kb on + strand, within ksgAat 52.062 kb on + strand, within ksgAat 52.062 kb on + strandat 52.070 kb on - strand, within ksgAat 52.072 kb on + strand, within ksgAat 52.089 kb on - strand, within ksgAat 52.276 kb on + strand, within ksgAat 52.276 kb on + strand, within ksgAat 52.284 kb on + strand, within ksgAat 52.338 kb on + strand, within ksgAat 52.338 kb on + strand, within ksgAat 52.343 kb on - strand, within ksgAat 52.348 kb on - strandat 52.348 kb on - strandat 52.348 kb on - strandat 52.354 kb on + strandat 52.420 kb on - strandat 52.490 kb on + strandat 52.490 kb on + strandat 52.502 kb on + strandat 52.502 kb on + strandat 52.518 kb on - strandat 52.518 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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50,361 + +0.2
50,361 + -0.0
50,370 - -0.6
50,376 + -0.3
50,461 + -1.2
50,469 - apaH b0049 0.11 -1.8
50,501 + apaH b0049 0.14 +0.5
50,507 - apaH b0049 0.15 +0.6
50,568 - apaH b0049 0.22 -1.2
50,717 - apaH b0049 0.40 -2.3
50,785 - apaH b0049 0.48 -0.5
50,791 + apaH b0049 0.49 +2.1
50,802 + apaH b0049 0.50 -0.2
50,829 - apaH b0049 0.53 +2.1
50,859 - apaH b0049 0.57 -1.4
50,877 + apaH b0049 0.59 +2.1
50,877 + apaH b0049 0.59 +2.1
51,025 + apaH b0049 0.77 -2.0
51,109 - apaH b0049 0.86 -2.4
51,177 + +0.5
51,177 + -1.2
51,185 - -2.2
51,190 + -1.8
51,190 + -2.1
51,240 + +0.9
51,240 + +1.6
51,311 + apaG b0050 0.22 +0.4
51,311 + apaG b0050 0.22 +1.1
51,322 - apaG b0050 0.25 +1.1
51,326 - apaG b0050 0.26 +1.1
51,334 - apaG b0050 0.28 +2.2
51,336 - apaG b0050 0.28 +0.9
51,353 + apaG b0050 0.33 +1.6
51,353 + apaG b0050 0.33 +0.7
51,353 - apaG b0050 0.33 +1.0
51,364 - apaG b0050 0.36 +0.8
51,372 - apaG b0050 0.38 +1.1
51,415 + apaG b0050 0.49 +1.2
51,423 - apaG b0050 0.51 +0.7
51,466 + apaG b0050 0.63 -0.2
51,472 + apaG b0050 0.64 +0.7
51,512 - apaG b0050 0.75 +0.9
51,514 - apaG b0050 0.75 +1.3
51,520 - apaG b0050 0.77 +0.1
51,524 + apaG b0050 0.78 +0.9
51,550 - apaG b0050 0.85 +1.0
51,560 + apaG b0050 0.88 +0.6
51,567 - apaG b0050 0.89 +1.4
51,567 - apaG b0050 0.89 +1.0
51,576 + +0.4
51,576 + +0.9
51,586 - +0.8
51,613 + +0.2
51,614 - -0.7
51,615 + -1.6
51,615 + -0.1
51,673 - -1.1
51,703 + ksgA b0051 0.11 +1.7
51,703 + ksgA b0051 0.11 +0.1
51,812 - ksgA b0051 0.25 +0.5
51,836 + ksgA b0051 0.28 +3.1
51,891 - ksgA b0051 0.34 +1.7
51,891 - ksgA b0051 0.34 -0.6
51,904 + ksgA b0051 0.36 +2.1
51,906 + ksgA b0051 0.36 -0.5
51,925 - ksgA b0051 0.38 -0.4
52,040 + ksgA b0051 0.52 +0.6
52,040 + ksgA b0051 0.52 -0.4
52,040 + ksgA b0051 0.52 +0.8
52,042 + ksgA b0051 0.53 -0.3
52,042 + ksgA b0051 0.53 +1.3
52,042 + ksgA b0051 0.53 -1.2
52,042 - ksgA b0051 0.53 -0.5
52,042 - ksgA b0051 0.53 +0.5
52,045 + ksgA b0051 0.53 -0.7
52,062 + ksgA b0051 0.55 +1.5
52,062 + +1.3
52,070 - ksgA b0051 0.56 -0.9
52,072 + ksgA b0051 0.56 +4.1
52,089 - ksgA b0051 0.58 -0.2
52,276 + ksgA b0051 0.81 -2.2
52,276 + ksgA b0051 0.81 -0.4
52,284 + ksgA b0051 0.82 -0.3
52,338 + ksgA b0051 0.89 -0.5
52,338 + ksgA b0051 0.89 +1.1
52,343 - ksgA b0051 0.89 +0.4
52,348 - -0.9
52,348 - -0.1
52,348 - +0.8
52,354 + +1.4
52,420 - -0.7
52,490 + +0.7
52,490 + +0.2
52,502 + -0.6
52,502 + +0.3
52,518 - +0.3
52,518 - -1.0

Or see this region's nucleotide sequence