Experiment: Nalidixic 0.06 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt topB and selD are separated by 4 nucleotides selD and ydjA are separated by 116 nucleotides ydjA and sppA are separated by 160 nucleotides
b1763: topB - DNA topoisomerase III (NCBI), at 1,843,023 to 1,844,984
topB
b1764: selD - selenophosphate synthetase (NCBI), at 1,844,989 to 1,846,032
selD
b1765: ydjA - predicted oxidoreductase (NCBI), at 1,846,149 to 1,846,700
ydjA
b1766: sppA - protease IV (signal peptide peptidase) (NCBI), at 1,846,861 to 1,848,717
sppA
Position (kb)
1844
1845
1846
1847 Strain fitness (log2 ratio)
-1
0
1 at 1844.351 kb on + strand, within topB at 1844.438 kb on + strand, within topB at 1844.443 kb on + strand, within topB at 1844.600 kb on - strand, within topB at 1844.829 kb on - strand at 1845.230 kb on - strand, within selD at 1845.242 kb on - strand, within selD at 1845.254 kb on - strand, within selD at 1845.300 kb on - strand, within selD at 1845.827 kb on - strand, within selD at 1845.827 kb on - strand, within selD at 1845.858 kb on - strand, within selD at 1845.881 kb on - strand, within selD at 1845.883 kb on - strand, within selD at 1846.260 kb on + strand, within ydjA at 1846.671 kb on - strand at 1846.671 kb on - strand at 1846.826 kb on - strand at 1846.828 kb on + strand at 1846.843 kb on - strand at 1846.849 kb on - strand at 1846.849 kb on - strand at 1846.860 kb on + strand at 1846.860 kb on + strand at 1846.910 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.06 mM remove 1,844,351 + topB b1763 0.68 -0.0 1,844,438 + topB b1763 0.72 -0.4 1,844,443 + topB b1763 0.72 -0.8 1,844,600 - topB b1763 0.80 -0.2 1,844,829 - +0.3 1,845,230 - selD b1764 0.23 -0.8 1,845,242 - selD b1764 0.24 -1.2 1,845,254 - selD b1764 0.25 -1.1 1,845,300 - selD b1764 0.30 -1.4 1,845,827 - selD b1764 0.80 -0.2 1,845,827 - selD b1764 0.80 -0.5 1,845,858 - selD b1764 0.83 -1.3 1,845,881 - selD b1764 0.85 -0.8 1,845,883 - selD b1764 0.86 -1.3 1,846,260 + ydjA b1765 0.20 -0.4 1,846,671 - -0.3 1,846,671 - +0.5 1,846,826 - -0.3 1,846,828 + -0.4 1,846,843 - -0.1 1,846,849 - +0.8 1,846,849 - +0.9 1,846,860 + +0.6 1,846,860 + -0.1 1,846,910 - +0.5
Or see this region's nucleotide sequence