Experiment: Ciprofloxacin Hydrochloride 0.0024 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mutS and pphB are separated by 105 nucleotides pphB and ygbI are separated by 50 nucleotides
b2733: mutS - DNA mismatch repair protein (NCBI), at 2,855,115 to 2,857,676
mutS
b2734: pphB - serine/threonine-specific protein phosphatase 2 (NCBI), at 2,857,782 to 2,858,438
pphB
b2735: ygbI - predicted DNA-binding transcriptional regulator (RefSeq), at 2,858,489 to 2,859,256
ygbI
Position (kb)
2857
2858
2859 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2856.799 kb on + strand, within mutS at 2856.799 kb on + strand, within mutS at 2856.804 kb on + strand, within mutS at 2856.812 kb on - strand, within mutS at 2856.817 kb on - strand, within mutS at 2856.986 kb on + strand, within mutS at 2856.999 kb on - strand, within mutS at 2857.001 kb on + strand, within mutS at 2857.021 kb on - strand, within mutS at 2857.089 kb on - strand, within mutS at 2857.308 kb on - strand, within mutS at 2857.378 kb on - strand, within mutS at 2857.378 kb on - strand, within mutS at 2857.408 kb on - strand, within mutS at 2857.408 kb on - strand, within mutS at 2857.453 kb on - strand at 2857.453 kb on - strand at 2857.512 kb on + strand at 2857.543 kb on + strand at 2857.566 kb on + strand at 2857.570 kb on - strand at 2857.575 kb on - strand at 2857.577 kb on + strand at 2857.591 kb on - strand at 2857.617 kb on + strand at 2857.621 kb on + strand at 2857.621 kb on + strand at 2857.621 kb on + strand at 2857.621 kb on + strand at 2857.621 kb on + strand at 2857.631 kb on + strand at 2857.649 kb on + strand at 2857.665 kb on + strand at 2857.665 kb on + strand at 2857.719 kb on + strand at 2857.743 kb on + strand at 2857.743 kb on + strand at 2857.747 kb on + strand at 2857.780 kb on - strand at 2857.784 kb on + strand at 2857.785 kb on - strand at 2857.786 kb on + strand at 2857.786 kb on + strand at 2857.787 kb on + strand at 2857.788 kb on + strand at 2857.794 kb on - strand at 2857.795 kb on - strand at 2857.829 kb on + strand at 2857.829 kb on + strand at 2857.838 kb on - strand at 2857.868 kb on + strand, within pphB at 2857.877 kb on - strand, within pphB at 2857.877 kb on - strand, within pphB at 2857.942 kb on + strand, within pphB at 2857.950 kb on - strand, within pphB at 2857.953 kb on - strand, within pphB at 2857.955 kb on - strand, within pphB at 2857.957 kb on + strand, within pphB at 2857.960 kb on - strand, within pphB at 2857.961 kb on - strand, within pphB at 2857.965 kb on + strand, within pphB at 2857.965 kb on - strand, within pphB at 2857.965 kb on - strand, within pphB at 2857.965 kb on - strand, within pphB at 2857.965 kb on - strand, within pphB at 2857.973 kb on - strand, within pphB at 2857.973 kb on - strand, within pphB at 2857.993 kb on - strand, within pphB at 2857.993 kb on - strand, within pphB at 2858.008 kb on - strand, within pphB at 2858.026 kb on + strand, within pphB at 2858.056 kb on + strand, within pphB at 2858.067 kb on + strand, within pphB at 2858.069 kb on + strand, within pphB at 2858.146 kb on + strand, within pphB at 2858.146 kb on + strand, within pphB at 2858.210 kb on + strand, within pphB at 2858.261 kb on - strand, within pphB at 2858.275 kb on - strand, within pphB at 2858.397 kb on - strand at 2858.405 kb on + strand at 2858.457 kb on + strand at 2858.457 kb on - strand at 2858.466 kb on - strand at 2858.471 kb on - strand at 2858.471 kb on - strand at 2858.473 kb on - strand at 2858.476 kb on - strand at 2858.514 kb on + strand at 2858.518 kb on + strand at 2858.568 kb on + strand, within ygbI at 2858.568 kb on + strand, within ygbI at 2858.618 kb on - strand, within ygbI at 2858.719 kb on + strand, within ygbI at 2858.822 kb on - strand, within ygbI at 2858.827 kb on - strand, within ygbI at 2858.827 kb on - strand, within ygbI at 2858.912 kb on - strand, within ygbI at 2859.114 kb on - strand, within ygbI at 2859.160 kb on - strand, within ygbI
Per-strain Table
Position Strand Gene LocusTag Fraction Ciprofloxacin Hydrochloride 0.0024 mM remove 2,856,799 + mutS b2733 0.66 -2.8 2,856,799 + mutS b2733 0.66 +0.5 2,856,804 + mutS b2733 0.66 +0.0 2,856,812 - mutS b2733 0.66 -3.2 2,856,817 - mutS b2733 0.66 -0.2 2,856,986 + mutS b2733 0.73 +1.4 2,856,999 - mutS b2733 0.74 -3.2 2,857,001 + mutS b2733 0.74 +0.2 2,857,021 - mutS b2733 0.74 +1.6 2,857,089 - mutS b2733 0.77 -0.2 2,857,308 - mutS b2733 0.86 -2.7 2,857,378 - mutS b2733 0.88 -1.8 2,857,378 - mutS b2733 0.88 +0.2 2,857,408 - mutS b2733 0.90 -2.0 2,857,408 - mutS b2733 0.90 +1.0 2,857,453 - -2.5 2,857,453 - +2.4 2,857,512 + +0.8 2,857,543 + +0.7 2,857,566 + -2.4 2,857,570 - -0.2 2,857,575 - +0.4 2,857,577 + -2.4 2,857,591 - +0.5 2,857,617 + -0.6 2,857,621 + +1.0 2,857,621 + +1.2 2,857,621 + -2.3 2,857,621 + +1.2 2,857,621 + -1.8 2,857,631 + -0.2 2,857,649 + -0.9 2,857,665 + -1.6 2,857,665 + +0.2 2,857,719 + +0.8 2,857,743 + -2.5 2,857,743 + -0.2 2,857,747 + -0.2 2,857,780 - -1.2 2,857,784 + +1.4 2,857,785 - +2.4 2,857,786 + +2.4 2,857,786 + +0.8 2,857,787 + +1.1 2,857,788 + +2.6 2,857,794 - -1.0 2,857,795 - -1.3 2,857,829 + +0.4 2,857,829 + +1.4 2,857,838 - +0.8 2,857,868 + pphB b2734 0.13 +2.4 2,857,877 - pphB b2734 0.14 -1.3 2,857,877 - pphB b2734 0.14 -0.2 2,857,942 + pphB b2734 0.24 +2.4 2,857,950 - pphB b2734 0.26 -0.4 2,857,953 - pphB b2734 0.26 -1.2 2,857,955 - pphB b2734 0.26 +0.8 2,857,957 + pphB b2734 0.27 +2.4 2,857,960 - pphB b2734 0.27 -0.7 2,857,961 - pphB b2734 0.27 +2.4 2,857,965 + pphB b2734 0.28 +2.4 2,857,965 - pphB b2734 0.28 -1.0 2,857,965 - pphB b2734 0.28 +1.4 2,857,965 - pphB b2734 0.28 -1.2 2,857,965 - pphB b2734 0.28 +1.6 2,857,973 - pphB b2734 0.29 -0.2 2,857,973 - pphB b2734 0.29 +0.4 2,857,993 - pphB b2734 0.32 +2.4 2,857,993 - pphB b2734 0.32 +0.1 2,858,008 - pphB b2734 0.34 -0.2 2,858,026 + pphB b2734 0.37 +1.4 2,858,056 + pphB b2734 0.42 +0.1 2,858,067 + pphB b2734 0.43 +2.4 2,858,069 + pphB b2734 0.44 +2.4 2,858,146 + pphB b2734 0.55 +2.4 2,858,146 + pphB b2734 0.55 +2.4 2,858,210 + pphB b2734 0.65 +1.4 2,858,261 - pphB b2734 0.73 +0.8 2,858,275 - pphB b2734 0.75 +0.8 2,858,397 - -0.6 2,858,405 + +0.3 2,858,457 + -0.4 2,858,457 - +1.1 2,858,466 - -2.8 2,858,471 - -0.8 2,858,471 - +0.1 2,858,473 - -0.2 2,858,476 - -0.6 2,858,514 + -0.2 2,858,518 + +3.1 2,858,568 + ygbI b2735 0.10 -0.5 2,858,568 + ygbI b2735 0.10 -1.9 2,858,618 - ygbI b2735 0.17 -3.1 2,858,719 + ygbI b2735 0.30 +1.4 2,858,822 - ygbI b2735 0.43 -0.2 2,858,827 - ygbI b2735 0.44 +2.4 2,858,827 - ygbI b2735 0.44 -1.1 2,858,912 - ygbI b2735 0.55 -0.9 2,859,114 - ygbI b2735 0.81 -0.7 2,859,160 - ygbI b2735 0.87 -1.0
Or see this region's nucleotide sequence