Experiment: Ciprofloxacin Hydrochloride 0.0024 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfiF and trxC are separated by 206 nucleotides trxC and yfiP are separated by 68 nucleotides yfiP and yfiQ are separated by 31 nucleotides
b2581: yfiF - predicted methyltransferase (NCBI), at 2,715,513 to 2,716,550
yfiF
b2582: trxC - thioredoxin 2 (NCBI), at 2,716,757 to 2,717,176
trxC
b2583: yfiP - orf, hypothetical protein (VIMSS), at 2,717,245 to 2,717,943
yfiP
b2584: yfiQ - fused predicted acyl-CoA synthetase: NAD(P)-binding subunit/ATP-binding subunit (NCBI), at 2,717,975 to 2,720,635
yfiQ
Position (kb)
2717
2718 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2716.265 kb on - strand, within yfiF at 2716.265 kb on - strand, within yfiF at 2716.313 kb on + strand, within yfiF at 2716.313 kb on + strand, within yfiF at 2716.387 kb on - strand, within yfiF at 2716.387 kb on - strand, within yfiF at 2716.440 kb on + strand, within yfiF at 2716.446 kb on + strand, within yfiF at 2716.485 kb on + strand at 2716.742 kb on + strand at 2716.762 kb on - strand at 2716.832 kb on + strand, within trxC at 2717.016 kb on - strand, within trxC at 2717.103 kb on + strand, within trxC at 2717.387 kb on - strand, within yfiP at 2717.387 kb on - strand, within yfiP at 2717.445 kb on + strand, within yfiP at 2717.445 kb on + strand, within yfiP at 2717.458 kb on - strand, within yfiP at 2717.591 kb on - strand, within yfiP at 2717.591 kb on - strand, within yfiP at 2717.605 kb on + strand, within yfiP at 2717.612 kb on + strand, within yfiP at 2717.612 kb on + strand, within yfiP at 2717.767 kb on - strand, within yfiP at 2717.817 kb on - strand, within yfiP at 2717.846 kb on + strand, within yfiP at 2717.846 kb on + strand, within yfiP at 2717.854 kb on - strand, within yfiP at 2717.902 kb on + strand at 2717.902 kb on + strand at 2717.979 kb on - strand at 2718.064 kb on - strand at 2718.151 kb on - strand at 2718.151 kb on - strand at 2718.309 kb on + strand, within yfiQ at 2718.337 kb on - strand, within yfiQ at 2718.461 kb on + strand, within yfiQ at 2718.469 kb on - strand, within yfiQ at 2718.471 kb on + strand, within yfiQ at 2718.481 kb on + strand, within yfiQ at 2718.534 kb on + strand, within yfiQ at 2718.534 kb on + strand, within yfiQ at 2718.597 kb on - strand, within yfiQ at 2718.681 kb on + strand, within yfiQ at 2718.773 kb on + strand, within yfiQ
Per-strain Table
Position Strand Gene LocusTag Fraction Ciprofloxacin Hydrochloride 0.0024 mM remove 2,716,265 - yfiF b2581 0.72 +0.0 2,716,265 - yfiF b2581 0.72 +0.8 2,716,313 + yfiF b2581 0.77 -0.5 2,716,313 + yfiF b2581 0.77 -0.8 2,716,387 - yfiF b2581 0.84 -1.9 2,716,387 - yfiF b2581 0.84 +1.5 2,716,440 + yfiF b2581 0.89 -0.8 2,716,446 + yfiF b2581 0.90 +0.3 2,716,485 + -0.7 2,716,742 + -0.6 2,716,762 - +0.9 2,716,832 + trxC b2582 0.18 -0.4 2,717,016 - trxC b2582 0.62 +0.6 2,717,103 + trxC b2582 0.82 +0.8 2,717,387 - yfiP b2583 0.20 +1.6 2,717,387 - yfiP b2583 0.20 +0.9 2,717,445 + yfiP b2583 0.29 -1.9 2,717,445 + yfiP b2583 0.29 +0.9 2,717,458 - yfiP b2583 0.30 -0.7 2,717,591 - yfiP b2583 0.49 +0.5 2,717,591 - yfiP b2583 0.49 +1.3 2,717,605 + yfiP b2583 0.52 +1.1 2,717,612 + yfiP b2583 0.53 +0.9 2,717,612 + yfiP b2583 0.53 -0.6 2,717,767 - yfiP b2583 0.75 +1.4 2,717,817 - yfiP b2583 0.82 +1.2 2,717,846 + yfiP b2583 0.86 +0.0 2,717,846 + yfiP b2583 0.86 +0.9 2,717,854 - yfiP b2583 0.87 -0.2 2,717,902 + -2.1 2,717,902 + -2.9 2,717,979 - +0.1 2,718,064 - -0.3 2,718,151 - +0.3 2,718,151 - +1.0 2,718,309 + yfiQ b2584 0.13 +0.1 2,718,337 - yfiQ b2584 0.14 -0.9 2,718,461 + yfiQ b2584 0.18 -0.4 2,718,469 - yfiQ b2584 0.19 +0.6 2,718,471 + yfiQ b2584 0.19 +0.7 2,718,481 + yfiQ b2584 0.19 +0.1 2,718,534 + yfiQ b2584 0.21 +2.1 2,718,534 + yfiQ b2584 0.21 -2.8 2,718,597 - yfiQ b2584 0.23 +0.9 2,718,681 + yfiQ b2584 0.27 +1.0 2,718,773 + yfiQ b2584 0.30 +1.6
Or see this region's nucleotide sequence