Strain Fitness in Escherichia coli BW25113 around b2577

Experiment: Ciprofloxacin Hydrochloride 0.0024 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsrmB and yfiE are separated by 208 nucleotidesyfiE and yfiK are separated by 102 nucleotidesyfiK and yfiD are separated by 55 nucleotides b2576: srmB - ATP-dependent RNA helicase (NCBI), at 2,710,918 to 2,712,252 srmB b2577: yfiE - putative transcriptional regulator LYSR-type (VIMSS), at 2,712,461 to 2,713,342 yfiE b2578: yfiK - neutral amino-acid efflux system (NCBI), at 2,713,445 to 2,714,032 yfiK b2579: yfiD - pyruvate formate lyase subunit (NCBI), at 2,714,088 to 2,714,471 yfiD Position (kb) 2712 2713 2714Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2711.557 kb on - strand, within srmBat 2711.666 kb on + strand, within srmBat 2711.698 kb on + strand, within srmBat 2711.747 kb on + strandat 2711.849 kb on + strand, within srmBat 2711.857 kb on - strand, within srmBat 2711.857 kb on - strand, within srmBat 2711.927 kb on + strand, within srmBat 2712.112 kb on - strand, within srmBat 2712.203 kb on + strandat 2712.218 kb on + strandat 2712.226 kb on - strandat 2712.489 kb on - strandat 2712.491 kb on - strandat 2712.491 kb on - strandat 2712.491 kb on - strandat 2712.533 kb on - strandat 2712.558 kb on - strand, within yfiEat 2712.620 kb on + strand, within yfiEat 2712.620 kb on + strand, within yfiEat 2712.653 kb on - strand, within yfiEat 2712.653 kb on - strand, within yfiEat 2712.745 kb on - strand, within yfiEat 2712.948 kb on - strand, within yfiEat 2713.040 kb on - strand, within yfiEat 2713.074 kb on - strand, within yfiEat 2713.329 kb on - strandat 2713.412 kb on + strandat 2713.412 kb on + strandat 2713.500 kb on + strandat 2713.508 kb on - strand, within yfiKat 2713.508 kb on - strand, within yfiKat 2713.525 kb on + strand, within yfiKat 2713.525 kb on + strand, within yfiKat 2713.533 kb on + strand, within yfiKat 2713.663 kb on + strand, within yfiKat 2713.692 kb on - strand, within yfiKat 2713.761 kb on + strand, within yfiKat 2713.815 kb on + strand, within yfiKat 2713.815 kb on + strand, within yfiKat 2713.815 kb on + strand, within yfiKat 2713.815 kb on + strand, within yfiKat 2713.833 kb on + strand, within yfiKat 2713.833 kb on + strand, within yfiKat 2714.065 kb on - strandat 2714.072 kb on + strandat 2714.072 kb on + strandat 2714.093 kb on + strandat 2714.105 kb on + strandat 2714.129 kb on + strand, within yfiDat 2714.220 kb on + strand, within yfiDat 2714.220 kb on + strand, within yfiDat 2714.255 kb on + strand, within yfiD

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Per-strain Table

Position Strand Gene LocusTag Fraction Ciprofloxacin Hydrochloride 0.0024 mM
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2,711,557 - srmB b2576 0.48 -1.2
2,711,666 + srmB b2576 0.56 +2.3
2,711,698 + srmB b2576 0.58 +3.0
2,711,747 + -0.2
2,711,849 + srmB b2576 0.70 +0.3
2,711,857 - srmB b2576 0.70 +1.9
2,711,857 - srmB b2576 0.70 +1.8
2,711,927 + srmB b2576 0.76 +1.0
2,712,112 - srmB b2576 0.89 +2.9
2,712,203 + -2.9
2,712,218 + -1.1
2,712,226 - -3.2
2,712,489 - +1.0
2,712,491 - -1.4
2,712,491 - +0.9
2,712,491 - +1.3
2,712,533 - -0.4
2,712,558 - yfiE b2577 0.11 +0.3
2,712,620 + yfiE b2577 0.18 +0.8
2,712,620 + yfiE b2577 0.18 +0.0
2,712,653 - yfiE b2577 0.22 +0.8
2,712,653 - yfiE b2577 0.22 +0.6
2,712,745 - yfiE b2577 0.32 +1.8
2,712,948 - yfiE b2577 0.55 +0.6
2,713,040 - yfiE b2577 0.66 +1.4
2,713,074 - yfiE b2577 0.70 +0.2
2,713,329 - +0.7
2,713,412 + -0.9
2,713,412 + +0.7
2,713,500 + -1.8
2,713,508 - yfiK b2578 0.11 +1.3
2,713,508 - yfiK b2578 0.11 -0.7
2,713,525 + yfiK b2578 0.14 +0.2
2,713,525 + yfiK b2578 0.14 -1.1
2,713,533 + yfiK b2578 0.15 -0.3
2,713,663 + yfiK b2578 0.37 -2.5
2,713,692 - yfiK b2578 0.42 +0.8
2,713,761 + yfiK b2578 0.54 -3.6
2,713,815 + yfiK b2578 0.63 -1.2
2,713,815 + yfiK b2578 0.63 +0.4
2,713,815 + yfiK b2578 0.63 +0.0
2,713,815 + yfiK b2578 0.63 -0.3
2,713,833 + yfiK b2578 0.66 +0.3
2,713,833 + yfiK b2578 0.66 +0.1
2,714,065 - -0.5
2,714,072 + +0.7
2,714,072 + -1.7
2,714,093 + -1.1
2,714,105 + -1.2
2,714,129 + yfiD b2579 0.11 -2.1
2,714,220 + yfiD b2579 0.34 -0.6
2,714,220 + yfiD b2579 0.34 -1.6
2,714,255 + yfiD b2579 0.43 +1.8

Or see this region's nucleotide sequence