Strain Fitness in Escherichia coli BW25113 around b2545

Experiment: Ciprofloxacin Hydrochloride 0.0024 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyphA and yphB are separated by 47 nucleotidesyphB and yphC are separated by 11 nucleotidesyphC and yphD are separated by 65 nucleotides b2543: yphA - predicted inner membrane protein (RefSeq), at 2,671,368 to 2,671,790 yphA b2544: yphB - hypothetical protein (NCBI), at 2,671,838 to 2,672,710 yphB b2545: yphC - putative oxidoreductase (VIMSS), at 2,672,722 to 2,673,783 yphC b2546: yphD - predicted sugar transporter subunit: membrane component of ABC superfamily (NCBI), at 2,673,849 to 2,674,847 yphD Position (kb) 2672 2673 2674Strain fitness (log2 ratio) -3 -2 -1 0 1at 2671.736 kb on + strand, within yphAat 2671.736 kb on + strand, within yphAat 2671.789 kb on + strandat 2671.890 kb on - strandat 2671.959 kb on - strand, within yphBat 2672.031 kb on - strand, within yphBat 2672.031 kb on - strand, within yphBat 2672.053 kb on + strand, within yphBat 2672.081 kb on + strand, within yphBat 2672.084 kb on - strand, within yphBat 2672.165 kb on - strand, within yphBat 2672.165 kb on - strand, within yphBat 2672.380 kb on - strand, within yphBat 2672.394 kb on + strand, within yphBat 2672.394 kb on + strand, within yphBat 2672.423 kb on + strand, within yphBat 2672.471 kb on - strand, within yphBat 2672.471 kb on - strand, within yphBat 2672.690 kb on + strandat 2672.697 kb on + strandat 2672.703 kb on - strandat 2672.704 kb on - strandat 2672.705 kb on - strandat 2672.764 kb on + strandat 2672.870 kb on + strand, within yphCat 2672.906 kb on - strand, within yphCat 2672.908 kb on + strand, within yphCat 2672.988 kb on + strand, within yphCat 2673.134 kb on - strand, within yphCat 2673.165 kb on + strand, within yphCat 2673.173 kb on + strand, within yphCat 2673.173 kb on + strand, within yphCat 2673.231 kb on + strand, within yphCat 2673.231 kb on + strand, within yphCat 2673.289 kb on + strand, within yphCat 2673.578 kb on - strand, within yphCat 2673.578 kb on - strand, within yphCat 2673.578 kb on - strand, within yphCat 2673.693 kb on + strandat 2673.800 kb on - strandat 2673.959 kb on + strand, within yphDat 2674.037 kb on + strand, within yphDat 2674.303 kb on - strand, within yphDat 2674.305 kb on + strand, within yphDat 2674.308 kb on - strand, within yphDat 2674.350 kb on + strand, within yphDat 2674.350 kb on + strand, within yphDat 2674.350 kb on + strand, within yphDat 2674.358 kb on - strand, within yphDat 2674.358 kb on - strand, within yphDat 2674.358 kb on - strand, within yphDat 2674.381 kb on + strand, within yphDat 2674.457 kb on + strand, within yphDat 2674.457 kb on + strand, within yphDat 2674.466 kb on - strand, within yphDat 2674.466 kb on - strand, within yphDat 2674.476 kb on - strand, within yphDat 2674.532 kb on - strand, within yphD

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Per-strain Table

Position Strand Gene LocusTag Fraction Ciprofloxacin Hydrochloride 0.0024 mM
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2,671,736 + yphA b2543 0.87 +0.1
2,671,736 + yphA b2543 0.87 -1.3
2,671,789 + -2.7
2,671,890 - -0.1
2,671,959 - yphB b2544 0.14 -0.2
2,672,031 - yphB b2544 0.22 -0.1
2,672,031 - yphB b2544 0.22 +1.1
2,672,053 + yphB b2544 0.25 -0.0
2,672,081 + yphB b2544 0.28 -0.6
2,672,084 - yphB b2544 0.28 -1.3
2,672,165 - yphB b2544 0.37 +1.3
2,672,165 - yphB b2544 0.37 -0.7
2,672,380 - yphB b2544 0.62 -1.0
2,672,394 + yphB b2544 0.64 -2.1
2,672,394 + yphB b2544 0.64 +0.3
2,672,423 + yphB b2544 0.67 +1.4
2,672,471 - yphB b2544 0.73 -1.2
2,672,471 - yphB b2544 0.73 +1.5
2,672,690 + +0.1
2,672,697 + -3.2
2,672,703 - -0.5
2,672,704 - -2.4
2,672,705 - -1.3
2,672,764 + -0.3
2,672,870 + yphC b2545 0.14 -3.7
2,672,906 - yphC b2545 0.17 -0.7
2,672,908 + yphC b2545 0.18 -0.5
2,672,988 + yphC b2545 0.25 +0.9
2,673,134 - yphC b2545 0.39 -0.3
2,673,165 + yphC b2545 0.42 -1.3
2,673,173 + yphC b2545 0.42 -0.6
2,673,173 + yphC b2545 0.42 +0.0
2,673,231 + yphC b2545 0.48 -0.0
2,673,231 + yphC b2545 0.48 -1.1
2,673,289 + yphC b2545 0.53 -0.4
2,673,578 - yphC b2545 0.81 -1.8
2,673,578 - yphC b2545 0.81 -3.0
2,673,578 - yphC b2545 0.81 -2.0
2,673,693 + -1.2
2,673,800 - -0.2
2,673,959 + yphD b2546 0.11 -2.8
2,674,037 + yphD b2546 0.19 +1.3
2,674,303 - yphD b2546 0.45 +0.6
2,674,305 + yphD b2546 0.46 -0.2
2,674,308 - yphD b2546 0.46 +0.9
2,674,350 + yphD b2546 0.50 -3.1
2,674,350 + yphD b2546 0.50 +1.7
2,674,350 + yphD b2546 0.50 -1.8
2,674,358 - yphD b2546 0.51 +0.2
2,674,358 - yphD b2546 0.51 -3.3
2,674,358 - yphD b2546 0.51 +0.1
2,674,381 + yphD b2546 0.53 +1.0
2,674,457 + yphD b2546 0.61 +0.8
2,674,457 + yphD b2546 0.61 +0.1
2,674,466 - yphD b2546 0.62 -2.7
2,674,466 - yphD b2546 0.62 +0.0
2,674,476 - yphD b2546 0.63 +1.7
2,674,532 - yphD b2546 0.68 -0.8

Or see this region's nucleotide sequence