Strain Fitness in Escherichia coli BW25113 around b1985

Experiment: Ciprofloxacin Hydrochloride 0.0024 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyeeN and asnW are separated by 452 nucleotidesasnW and yeeO are separated by 100 nucleotidesyeeO and asnU are separated by 160 nucleotidesasnU and cbl are separated by 37 nucleotides b1983: yeeN - hypothetical protein (NCBI), at 2,054,882 to 2,055,598 yeeN b1984: asnW - tRNA-Asn (NCBI), at 2,056,051 to 2,056,126 asnW b1985: yeeO - predicted multidrug efflux system (RefSeq), at 2,056,227 to 2,057,714 yeeO b1986: asnU - tRNA-Asn (NCBI), at 2,057,875 to 2,057,950 asnU b1987: cbl - DNA-binding transcriptional activator of cysteine biosynthesis (NCBI), at 2,057,988 to 2,058,938 cbl Position (kb) 2056 2057 2058Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2055.250 kb on + strand, within yeeNat 2055.262 kb on + strand, within yeeNat 2055.262 kb on + strand, within yeeNat 2055.264 kb on + strand, within yeeNat 2055.264 kb on + strand, within yeeNat 2055.264 kb on + strand, within yeeNat 2055.267 kb on + strand, within yeeNat 2055.277 kb on + strand, within yeeNat 2055.309 kb on + strand, within yeeNat 2055.309 kb on + strand, within yeeNat 2055.354 kb on + strand, within yeeNat 2055.354 kb on + strand, within yeeNat 2055.354 kb on + strand, within yeeNat 2055.364 kb on + strand, within yeeNat 2055.364 kb on + strand, within yeeNat 2055.364 kb on + strand, within yeeNat 2055.412 kb on + strand, within yeeNat 2055.420 kb on - strand, within yeeNat 2055.430 kb on + strand, within yeeNat 2055.430 kb on + strand, within yeeNat 2055.443 kb on + strand, within yeeNat 2055.443 kb on + strand, within yeeNat 2055.454 kb on + strand, within yeeNat 2055.487 kb on + strand, within yeeNat 2055.502 kb on + strand, within yeeNat 2055.522 kb on + strand, within yeeNat 2055.543 kb on + strandat 2055.543 kb on + strandat 2055.543 kb on + strandat 2055.782 kb on - strandat 2055.852 kb on - strandat 2055.857 kb on - strandat 2055.857 kb on - strandat 2055.877 kb on - strandat 2055.887 kb on + strandat 2055.889 kb on + strandat 2055.895 kb on - strandat 2055.895 kb on - strandat 2055.895 kb on - strandat 2055.897 kb on - strandat 2055.930 kb on - strandat 2056.031 kb on - strandat 2056.031 kb on - strandat 2056.032 kb on - strandat 2056.075 kb on - strand, within asnWat 2056.312 kb on - strandat 2056.322 kb on - strandat 2056.322 kb on - strandat 2056.326 kb on + strandat 2056.445 kb on + strand, within yeeOat 2056.480 kb on + strand, within yeeOat 2056.557 kb on + strand, within yeeOat 2056.702 kb on - strand, within yeeOat 2056.725 kb on - strand, within yeeOat 2056.725 kb on - strand, within yeeOat 2056.738 kb on - strand, within yeeOat 2056.738 kb on - strand, within yeeOat 2056.750 kb on - strand, within yeeOat 2056.758 kb on + strand, within yeeOat 2056.758 kb on + strand, within yeeOat 2056.807 kb on - strand, within yeeOat 2056.857 kb on + strand, within yeeOat 2056.857 kb on + strand, within yeeOat 2056.880 kb on + strand, within yeeOat 2056.893 kb on + strand, within yeeOat 2056.895 kb on + strand, within yeeOat 2056.895 kb on + strand, within yeeOat 2057.027 kb on + strand, within yeeOat 2057.027 kb on + strand, within yeeOat 2057.033 kb on + strand, within yeeOat 2057.035 kb on - strand, within yeeOat 2057.120 kb on - strand, within yeeOat 2057.151 kb on - strand, within yeeOat 2057.181 kb on - strand, within yeeOat 2057.181 kb on - strand, within yeeOat 2057.206 kb on - strand, within yeeOat 2057.213 kb on - strand, within yeeOat 2057.213 kb on - strand, within yeeOat 2057.232 kb on - strand, within yeeOat 2057.367 kb on - strand, within yeeOat 2057.585 kb on + strandat 2057.849 kb on + strandat 2058.044 kb on + strandat 2058.057 kb on - strandat 2058.057 kb on - strandat 2058.093 kb on + strand, within cblat 2058.094 kb on - strand, within cblat 2058.096 kb on - strand, within cblat 2058.096 kb on - strand, within cblat 2058.123 kb on + strand, within cblat 2058.218 kb on - strand, within cblat 2058.275 kb on + strand, within cblat 2058.575 kb on - strand, within cblat 2058.618 kb on + strand, within cblat 2058.644 kb on + strand, within cbl

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Per-strain Table

Position Strand Gene LocusTag Fraction Ciprofloxacin Hydrochloride 0.0024 mM
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2,055,250 + yeeN b1983 0.51 -1.1
2,055,262 + yeeN b1983 0.53 -0.3
2,055,262 + yeeN b1983 0.53 +2.0
2,055,264 + yeeN b1983 0.53 -0.9
2,055,264 + yeeN b1983 0.53 -0.5
2,055,264 + yeeN b1983 0.53 +0.3
2,055,267 + yeeN b1983 0.54 -0.7
2,055,277 + yeeN b1983 0.55 -1.0
2,055,309 + yeeN b1983 0.60 +0.7
2,055,309 + yeeN b1983 0.60 +2.3
2,055,354 + yeeN b1983 0.66 +0.3
2,055,354 + yeeN b1983 0.66 +2.3
2,055,354 + yeeN b1983 0.66 +3.2
2,055,364 + yeeN b1983 0.67 +2.3
2,055,364 + yeeN b1983 0.67 +2.7
2,055,364 + yeeN b1983 0.67 +0.7
2,055,412 + yeeN b1983 0.74 +0.7
2,055,420 - yeeN b1983 0.75 +2.3
2,055,430 + yeeN b1983 0.76 -0.0
2,055,430 + yeeN b1983 0.76 -0.0
2,055,443 + yeeN b1983 0.78 -0.7
2,055,443 + yeeN b1983 0.78 +2.4
2,055,454 + yeeN b1983 0.80 -1.1
2,055,487 + yeeN b1983 0.84 +0.0
2,055,502 + yeeN b1983 0.86 +1.7
2,055,522 + yeeN b1983 0.89 -3.1
2,055,543 + -0.1
2,055,543 + -1.6
2,055,543 + -0.7
2,055,782 - +0.5
2,055,852 - -1.1
2,055,857 - -1.5
2,055,857 - +0.3
2,055,877 - -0.7
2,055,887 + -0.3
2,055,889 + -0.0
2,055,895 - +0.5
2,055,895 - +0.3
2,055,895 - +0.9
2,055,897 - +0.3
2,055,930 - -0.7
2,056,031 - +0.4
2,056,031 - +1.6
2,056,032 - -1.9
2,056,075 - asnW b1984 0.32 +0.3
2,056,312 - +1.3
2,056,322 - -0.9
2,056,322 - +0.9
2,056,326 + +2.3
2,056,445 + yeeO b1985 0.15 -1.7
2,056,480 + yeeO b1985 0.17 -1.9
2,056,557 + yeeO b1985 0.22 +1.6
2,056,702 - yeeO b1985 0.32 -3.8
2,056,725 - yeeO b1985 0.33 -0.7
2,056,725 - yeeO b1985 0.33 +0.2
2,056,738 - yeeO b1985 0.34 +0.3
2,056,738 - yeeO b1985 0.34 +0.1
2,056,750 - yeeO b1985 0.35 -2.4
2,056,758 + yeeO b1985 0.36 -0.5
2,056,758 + yeeO b1985 0.36 -0.0
2,056,807 - yeeO b1985 0.39 -0.2
2,056,857 + yeeO b1985 0.42 +2.3
2,056,857 + yeeO b1985 0.42 -0.8
2,056,880 + yeeO b1985 0.44 -2.9
2,056,893 + yeeO b1985 0.45 -0.2
2,056,895 + yeeO b1985 0.45 +0.3
2,056,895 + yeeO b1985 0.45 +0.7
2,057,027 + yeeO b1985 0.54 +0.3
2,057,027 + yeeO b1985 0.54 -0.0
2,057,033 + yeeO b1985 0.54 +0.5
2,057,035 - yeeO b1985 0.54 -3.8
2,057,120 - yeeO b1985 0.60 +0.1
2,057,151 - yeeO b1985 0.62 -0.3
2,057,181 - yeeO b1985 0.64 -1.9
2,057,181 - yeeO b1985 0.64 -0.6
2,057,206 - yeeO b1985 0.66 -0.3
2,057,213 - yeeO b1985 0.66 -2.1
2,057,213 - yeeO b1985 0.66 +0.3
2,057,232 - yeeO b1985 0.68 -2.8
2,057,367 - yeeO b1985 0.77 -2.3
2,057,585 + +0.3
2,057,849 + -0.1
2,058,044 + +0.4
2,058,057 - +0.7
2,058,057 - +1.0
2,058,093 + cbl b1987 0.11 -2.7
2,058,094 - cbl b1987 0.11 +0.3
2,058,096 - cbl b1987 0.11 +1.1
2,058,096 - cbl b1987 0.11 -1.7
2,058,123 + cbl b1987 0.14 -1.7
2,058,218 - cbl b1987 0.24 -1.2
2,058,275 + cbl b1987 0.30 -2.4
2,058,575 - cbl b1987 0.62 -0.1
2,058,618 + cbl b1987 0.66 -0.1
2,058,644 + cbl b1987 0.69 +0.1

Or see this region's nucleotide sequence