Experiment: Ciprofloxacin Hydrochloride 0.0024 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b1592 and b1593 overlap by 48 nucleotides b1593 and dgsA are separated by 124 nucleotides dgsA and ynfL are separated by 134 nucleotides
b1592: b1592 - putative chloride channel (VIMSS), at 1,663,339 to 1,664,595
b1592
b1593: b1593 - orf, hypothetical protein (VIMSS), at 1,664,548 to 1,665,243
b1593
b1594: dgsA - DNA-binding transcriptional repressor (NCBI), at 1,665,368 to 1,666,588
dgsA
b1595: ynfL - predicted DNA-binding transcriptional regulator (NCBI), at 1,666,723 to 1,667,616
ynfL
Position (kb)
1665
1666
1667 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1664.448 kb on + strand, within b1592 at 1664.448 kb on + strand, within b1592 at 1664.579 kb on - strand at 1664.586 kb on + strand at 1664.586 kb on + strand at 1664.976 kb on + strand, within b1593 at 1664.983 kb on + strand, within b1593 at 1664.983 kb on + strand, within b1593 at 1664.991 kb on - strand, within b1593 at 1664.991 kb on - strand, within b1593 at 1664.991 kb on - strand, within b1593 at 1665.407 kb on - strand at 1665.479 kb on - strand at 1665.479 kb on - strand at 1665.479 kb on - strand at 1665.479 kb on - strand at 1665.493 kb on - strand, within dgsA at 1665.645 kb on - strand, within dgsA at 1665.645 kb on - strand, within dgsA at 1665.781 kb on - strand, within dgsA at 1665.781 kb on - strand, within dgsA at 1665.898 kb on + strand, within dgsA at 1665.919 kb on + strand, within dgsA at 1665.919 kb on + strand, within dgsA at 1666.141 kb on - strand, within dgsA at 1666.300 kb on + strand, within dgsA at 1666.300 kb on + strand, within dgsA at 1666.471 kb on - strand at 1666.515 kb on - strand at 1666.726 kb on + strand at 1666.726 kb on + strand at 1666.732 kb on - strand at 1666.732 kb on - strand at 1667.012 kb on - strand, within ynfL at 1667.263 kb on - strand, within ynfL at 1667.263 kb on - strand, within ynfL at 1667.440 kb on + strand, within ynfL at 1667.445 kb on + strand, within ynfL at 1667.445 kb on + strand, within ynfL
Per-strain Table
Position Strand Gene LocusTag Fraction Ciprofloxacin Hydrochloride 0.0024 mM remove 1,664,448 + b1592 b1592 0.88 +0.3 1,664,448 + b1592 b1592 0.88 -1.5 1,664,579 - -0.1 1,664,586 + -1.4 1,664,586 + +0.9 1,664,976 + b1593 b1593 0.61 -0.1 1,664,983 + b1593 b1593 0.62 -0.9 1,664,983 + b1593 b1593 0.62 -0.5 1,664,991 - b1593 b1593 0.64 +0.9 1,664,991 - b1593 b1593 0.64 -0.9 1,664,991 - b1593 b1593 0.64 -0.2 1,665,407 - +1.1 1,665,479 - +1.3 1,665,479 - +0.5 1,665,479 - -2.1 1,665,479 - -0.1 1,665,493 - dgsA b1594 0.10 -3.3 1,665,645 - dgsA b1594 0.23 +0.3 1,665,645 - dgsA b1594 0.23 +1.4 1,665,781 - dgsA b1594 0.34 -0.0 1,665,781 - dgsA b1594 0.34 -3.4 1,665,898 + dgsA b1594 0.43 -1.0 1,665,919 + dgsA b1594 0.45 -0.0 1,665,919 + dgsA b1594 0.45 +0.7 1,666,141 - dgsA b1594 0.63 -0.3 1,666,300 + dgsA b1594 0.76 -0.5 1,666,300 + dgsA b1594 0.76 -0.5 1,666,471 - -2.1 1,666,515 - -1.0 1,666,726 + +1.4 1,666,726 + -1.5 1,666,732 - +0.8 1,666,732 - +1.8 1,667,012 - ynfL b1595 0.32 +1.3 1,667,263 - ynfL b1595 0.60 +1.7 1,667,263 - ynfL b1595 0.60 -1.3 1,667,440 + ynfL b1595 0.80 -0.5 1,667,445 + ynfL b1595 0.81 +2.3 1,667,445 + ynfL b1595 0.81 -0.5
Or see this region's nucleotide sequence