Strain Fitness in Escherichia coli BW25113 around b1322

Experiment: Ciprofloxacin Hydrochloride 0.0024 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycjX and ycjF overlap by 4 nucleotidesycjF and tyrR are separated by 147 nucleotides b1321: ycjX - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 1,382,141 to 1,383,538 ycjX b1322: ycjF - hypothetical protein (NCBI), at 1,383,535 to 1,384,596 ycjF b1323: tyrR - DNA-binding transcriptional dual regulator, tyrosine-binding (NCBI), at 1,384,744 to 1,386,285 tyrR Position (kb) 1383 1384 1385Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1382.625 kb on - strand, within ycjXat 1382.756 kb on - strand, within ycjXat 1382.767 kb on - strand, within ycjXat 1382.860 kb on - strand, within ycjXat 1382.860 kb on - strand, within ycjXat 1383.086 kb on + strand, within ycjXat 1383.211 kb on - strand, within ycjXat 1383.269 kb on - strand, within ycjXat 1383.365 kb on - strand, within ycjXat 1383.365 kb on - strand, within ycjXat 1383.367 kb on - strand, within ycjXat 1383.572 kb on + strandat 1383.781 kb on + strand, within ycjFat 1383.801 kb on - strand, within ycjFat 1383.801 kb on - strand, within ycjFat 1383.828 kb on - strand, within ycjFat 1383.892 kb on + strand, within ycjFat 1383.892 kb on + strand, within ycjFat 1383.937 kb on - strand, within ycjFat 1384.032 kb on + strand, within ycjFat 1384.032 kb on + strand, within ycjFat 1384.214 kb on + strand, within ycjFat 1384.214 kb on + strand, within ycjFat 1384.420 kb on + strand, within ycjFat 1384.428 kb on - strand, within ycjFat 1384.428 kb on - strand, within ycjFat 1384.428 kb on - strand, within ycjFat 1384.941 kb on + strand, within tyrRat 1384.949 kb on - strand, within tyrRat 1385.018 kb on - strand, within tyrRat 1385.154 kb on + strand, within tyrRat 1385.222 kb on + strand, within tyrRat 1385.222 kb on + strand, within tyrR

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Per-strain Table

Position Strand Gene LocusTag Fraction Ciprofloxacin Hydrochloride 0.0024 mM
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1,382,625 - ycjX b1321 0.35 -1.6
1,382,756 - ycjX b1321 0.44 -3.1
1,382,767 - ycjX b1321 0.45 +1.3
1,382,860 - ycjX b1321 0.51 -0.8
1,382,860 - ycjX b1321 0.51 -0.5
1,383,086 + ycjX b1321 0.68 -0.3
1,383,211 - ycjX b1321 0.77 +0.1
1,383,269 - ycjX b1321 0.81 -0.3
1,383,365 - ycjX b1321 0.88 +0.1
1,383,365 - ycjX b1321 0.88 -1.3
1,383,367 - ycjX b1321 0.88 +0.7
1,383,572 + +1.3
1,383,781 + ycjF b1322 0.23 -1.0
1,383,801 - ycjF b1322 0.25 -1.5
1,383,801 - ycjF b1322 0.25 -3.3
1,383,828 - ycjF b1322 0.28 -0.4
1,383,892 + ycjF b1322 0.34 +3.5
1,383,892 + ycjF b1322 0.34 -0.7
1,383,937 - ycjF b1322 0.38 -0.1
1,384,032 + ycjF b1322 0.47 -2.3
1,384,032 + ycjF b1322 0.47 +0.1
1,384,214 + ycjF b1322 0.64 -0.5
1,384,214 + ycjF b1322 0.64 -2.0
1,384,420 + ycjF b1322 0.83 -0.9
1,384,428 - ycjF b1322 0.84 -1.4
1,384,428 - ycjF b1322 0.84 +0.2
1,384,428 - ycjF b1322 0.84 -2.9
1,384,941 + tyrR b1323 0.13 +0.1
1,384,949 - tyrR b1323 0.13 -0.1
1,385,018 - tyrR b1323 0.18 +1.9
1,385,154 + tyrR b1323 0.27 -2.2
1,385,222 + tyrR b1323 0.31 +0.0
1,385,222 + tyrR b1323 0.31 -0.0

Or see this region's nucleotide sequence