Strain Fitness in Escherichia coli BW25113 around b0974

Experiment: Ciprofloxacin Hydrochloride 0.0024 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthyaB and hyaC are separated by 18 nucleotideshyaC and hyaD overlap by 4 nucleotideshyaD and hyaE overlap by 4 nucleotideshyaE and hyaF overlap by 4 nucleotides b0973: hyaB - hydrogenase 1, large subunit (NCBI), at 1,032,477 to 1,034,270 hyaB b0974: hyaC - hydrogenase 1, b-type cytochrome subunit (NCBI), at 1,034,289 to 1,034,996 hyaC b0975: hyaD - protein involved in processing of HyaA and HyaB proteins (NCBI), at 1,034,993 to 1,035,580 hyaD b0976: hyaE - protein involved in processing of HyaA and HyaB proteins (NCBI), at 1,035,577 to 1,035,975 hyaE b0977: hyaF - protein involved in nickel incorporation into hydrogenase-1 proteins (NCBI), at 1,035,972 to 1,036,829 hyaF Position (kb) 1034 1035Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1033.392 kb on - strand, within hyaBat 1033.419 kb on + strand, within hyaBat 1033.419 kb on + strand, within hyaBat 1033.517 kb on - strand, within hyaBat 1033.517 kb on - strand, within hyaBat 1033.529 kb on + strand, within hyaBat 1033.742 kb on + strand, within hyaBat 1033.742 kb on + strand, within hyaBat 1033.875 kb on - strand, within hyaBat 1033.960 kb on + strand, within hyaBat 1033.960 kb on + strand, within hyaBat 1034.059 kb on + strand, within hyaBat 1034.059 kb on + strand, within hyaBat 1034.191 kb on + strandat 1034.236 kb on - strandat 1034.252 kb on - strandat 1034.276 kb on + strandat 1034.276 kb on + strandat 1034.319 kb on + strandat 1034.319 kb on + strandat 1034.408 kb on - strand, within hyaCat 1034.577 kb on - strand, within hyaCat 1034.720 kb on - strand, within hyaCat 1034.720 kb on - strand, within hyaCat 1034.890 kb on - strand, within hyaCat 1034.895 kb on - strand, within hyaCat 1034.940 kb on + strandat 1034.940 kb on + strandat 1034.945 kb on + strandat 1034.947 kb on - strandat 1035.102 kb on + strand, within hyaDat 1035.201 kb on + strand, within hyaDat 1035.201 kb on + strand, within hyaDat 1035.291 kb on - strand, within hyaDat 1035.291 kb on - strand, within hyaDat 1035.310 kb on + strand, within hyaDat 1035.437 kb on + strand, within hyaDat 1035.437 kb on + strand, within hyaDat 1035.495 kb on + strand, within hyaDat 1035.503 kb on - strand, within hyaDat 1035.503 kb on - strand, within hyaDat 1035.803 kb on + strand, within hyaEat 1035.813 kb on + strand, within hyaEat 1035.892 kb on + strand, within hyaEat 1035.910 kb on - strand, within hyaEat 1035.910 kb on - strand, within hyaEat 1035.990 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ciprofloxacin Hydrochloride 0.0024 mM
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1,033,392 - hyaB b0973 0.51 -0.3
1,033,419 + hyaB b0973 0.53 -2.8
1,033,419 + hyaB b0973 0.53 -0.5
1,033,517 - hyaB b0973 0.58 -2.5
1,033,517 - hyaB b0973 0.58 +0.8
1,033,529 + hyaB b0973 0.59 -1.9
1,033,742 + hyaB b0973 0.71 -1.2
1,033,742 + hyaB b0973 0.71 +2.5
1,033,875 - hyaB b0973 0.78 +1.8
1,033,960 + hyaB b0973 0.83 -1.9
1,033,960 + hyaB b0973 0.83 +0.3
1,034,059 + hyaB b0973 0.88 +1.2
1,034,059 + hyaB b0973 0.88 +0.3
1,034,191 + +0.3
1,034,236 - +1.5
1,034,252 - -0.7
1,034,276 + +1.2
1,034,276 + +1.3
1,034,319 + -2.2
1,034,319 + +1.5
1,034,408 - hyaC b0974 0.17 -3.2
1,034,577 - hyaC b0974 0.41 -0.6
1,034,720 - hyaC b0974 0.61 -1.5
1,034,720 - hyaC b0974 0.61 -0.3
1,034,890 - hyaC b0974 0.85 -0.1
1,034,895 - hyaC b0974 0.86 -1.4
1,034,940 + +0.6
1,034,940 + -1.9
1,034,945 + +0.9
1,034,947 - +0.5
1,035,102 + hyaD b0975 0.19 +0.3
1,035,201 + hyaD b0975 0.35 +1.0
1,035,201 + hyaD b0975 0.35 -1.1
1,035,291 - hyaD b0975 0.51 +0.2
1,035,291 - hyaD b0975 0.51 +1.8
1,035,310 + hyaD b0975 0.54 +0.6
1,035,437 + hyaD b0975 0.76 -0.9
1,035,437 + hyaD b0975 0.76 -0.1
1,035,495 + hyaD b0975 0.85 +0.7
1,035,503 - hyaD b0975 0.87 +1.5
1,035,503 - hyaD b0975 0.87 +1.1
1,035,803 + hyaE b0976 0.57 -0.0
1,035,813 + hyaE b0976 0.59 -2.4
1,035,892 + hyaE b0976 0.79 +0.6
1,035,910 - hyaE b0976 0.83 -2.5
1,035,910 - hyaE b0976 0.83 -2.1
1,035,990 + +0.7

Or see this region's nucleotide sequence