Strain Fitness in Escherichia coli BW25113 around b0937

Experiment: Ciprofloxacin Hydrochloride 0.0024 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntssuD and ycbO overlap by 4 nucleotidesycbO and ssuE overlap by 8 nucleotidesssuE and ycbQ are separated by 355 nucleotidesycbQ and ycbR are separated by 82 nucleotides b0935: ssuD - alkanesulfonate monooxygenase (NCBI), at 994,066 to 995,211 ssuD b0936: ycbO - orf, hypothetical protein (VIMSS), at 995,208 to 996,167 ycbO b0937: ssuE - NAD(P)H-dependent FMN reductase (NCBI), at 996,160 to 996,735 ssuE b0938: ycbQ - putative fimbrial-like protein (VIMSS), at 997,091 to 997,630 ycbQ b0939: ycbR - predicted periplasmic pilin chaperone (NCBI), at 997,713 to 998,414 ycbR Position (kb) 996 997Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 995.236 kb on - strandat 995.236 kb on - strandat 995.473 kb on - strand, within ycbOat 995.512 kb on + strand, within ycbOat 995.607 kb on + strand, within ycbOat 995.616 kb on + strand, within ycbOat 995.631 kb on + strand, within ycbOat 995.734 kb on - strand, within ycbOat 995.753 kb on - strand, within ycbOat 995.832 kb on - strand, within ycbOat 995.871 kb on + strand, within ycbOat 995.871 kb on + strand, within ycbOat 996.185 kb on + strandat 996.207 kb on - strandat 996.267 kb on + strand, within ssuEat 996.350 kb on - strand, within ssuEat 996.637 kb on + strand, within ssuEat 996.637 kb on + strand, within ssuEat 996.710 kb on + strandat 996.710 kb on + strandat 996.714 kb on + strandat 996.718 kb on - strandat 996.721 kb on + strandat 996.721 kb on + strandat 996.722 kb on - strandat 996.743 kb on + strandat 996.743 kb on + strandat 996.751 kb on - strandat 996.774 kb on + strandat 996.793 kb on + strandat 996.793 kb on + strandat 996.797 kb on + strandat 996.797 kb on + strandat 997.121 kb on + strandat 997.152 kb on - strand, within ycbQat 997.152 kb on - strand, within ycbQat 997.191 kb on - strand, within ycbQat 997.257 kb on + strand, within ycbQat 997.347 kb on - strand, within ycbQat 997.347 kb on - strand, within ycbQat 997.347 kb on - strand, within ycbQat 997.374 kb on + strand, within ycbQat 997.390 kb on + strand, within ycbQat 997.402 kb on + strand, within ycbQat 997.423 kb on + strand, within ycbQat 997.423 kb on + strand, within ycbQat 997.424 kb on + strand, within ycbQat 997.429 kb on + strand, within ycbQat 997.454 kb on + strand, within ycbQat 997.454 kb on + strand, within ycbQat 997.454 kb on + strand, within ycbQat 997.454 kb on + strand, within ycbQat 997.485 kb on - strand, within ycbQat 997.486 kb on + strand, within ycbQat 997.486 kb on + strand, within ycbQat 997.525 kb on + strand, within ycbQat 997.525 kb on + strand, within ycbQat 997.545 kb on + strand, within ycbQat 997.566 kb on + strand, within ycbQat 997.573 kb on + strand, within ycbQat 997.573 kb on + strand, within ycbQat 997.573 kb on + strand, within ycbQat 997.586 kb on + strandat 997.586 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ciprofloxacin Hydrochloride 0.0024 mM
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995,236 - -0.8
995,236 - +0.8
995,473 - ycbO b0936 0.28 -0.0
995,512 + ycbO b0936 0.32 -0.0
995,607 + ycbO b0936 0.42 -0.3
995,616 + ycbO b0936 0.42 +0.7
995,631 + ycbO b0936 0.44 -0.1
995,734 - ycbO b0936 0.55 +0.2
995,753 - ycbO b0936 0.57 +0.2
995,832 - ycbO b0936 0.65 -0.6
995,871 + ycbO b0936 0.69 +0.9
995,871 + ycbO b0936 0.69 -0.9
996,185 + -1.2
996,207 - -0.3
996,267 + ssuE b0937 0.19 -0.6
996,350 - ssuE b0937 0.33 -1.0
996,637 + ssuE b0937 0.83 -0.0
996,637 + ssuE b0937 0.83 +0.5
996,710 + -0.9
996,710 + +1.6
996,714 + -1.9
996,718 - -1.0
996,721 + -0.6
996,721 + +1.0
996,722 - +0.7
996,743 + +1.6
996,743 + -2.0
996,751 - +0.5
996,774 + -0.8
996,793 + -0.4
996,793 + -0.3
996,797 + -2.4
996,797 + -0.2
997,121 + +2.5
997,152 - ycbQ b0938 0.11 +2.5
997,152 - ycbQ b0938 0.11 +2.5
997,191 - ycbQ b0938 0.19 +2.5
997,257 + ycbQ b0938 0.31 +2.5
997,347 - ycbQ b0938 0.47 +2.5
997,347 - ycbQ b0938 0.47 +2.5
997,347 - ycbQ b0938 0.47 +2.5
997,374 + ycbQ b0938 0.52 +1.5
997,390 + ycbQ b0938 0.55 -1.6
997,402 + ycbQ b0938 0.58 +2.5
997,423 + ycbQ b0938 0.61 +4.2
997,423 + ycbQ b0938 0.61 +3.9
997,424 + ycbQ b0938 0.62 -0.9
997,429 + ycbQ b0938 0.63 +0.9
997,454 + ycbQ b0938 0.67 -1.2
997,454 + ycbQ b0938 0.67 +1.5
997,454 + ycbQ b0938 0.67 +0.9
997,454 + ycbQ b0938 0.67 -1.1
997,485 - ycbQ b0938 0.73 +2.5
997,486 + ycbQ b0938 0.73 +2.4
997,486 + ycbQ b0938 0.73 +2.2
997,525 + ycbQ b0938 0.80 -1.1
997,525 + ycbQ b0938 0.80 +1.0
997,545 + ycbQ b0938 0.84 -0.2
997,566 + ycbQ b0938 0.88 -1.0
997,573 + ycbQ b0938 0.89 -1.4
997,573 + ycbQ b0938 0.89 -1.6
997,573 + ycbQ b0938 0.89 -0.2
997,586 + +1.8
997,586 + -0.3

Or see this region's nucleotide sequence