Strain Fitness in Escherichia coli BW25113 around b0810

Experiment: Ciprofloxacin Hydrochloride 0.0024 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybiO and glnQ are separated by 260 nucleotidesglnQ and glnP overlap by 4 nucleotidesglnP and glnH are separated by 138 nucleotides b0808: ybiO - putative transport protein (VIMSS), at 842,478 to 844,703 ybiO b0809: glnQ - glutamine ABC transporter ATP-binding protein (NCBI), at 844,964 to 845,686 glnQ b0810: glnP - glutamine ABC transporter permease protein (NCBI), at 845,683 to 846,342 glnP b0811: glnH - glutamine ABC transporter periplasmic protein (NCBI), at 846,481 to 847,227 glnH Position (kb) 845 846 847Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 844.694 kb on - strandat 844.712 kb on - strandat 845.109 kb on + strand, within glnQat 845.154 kb on + strand, within glnQat 845.243 kb on + strand, within glnQat 845.243 kb on + strand, within glnQat 845.288 kb on + strand, within glnQat 845.547 kb on - strand, within glnQat 845.569 kb on + strand, within glnQat 845.596 kb on - strand, within glnQat 845.596 kb on - strand, within glnQat 845.994 kb on + strand, within glnPat 845.994 kb on + strand, within glnPat 845.997 kb on - strand, within glnPat 845.998 kb on + strand, within glnPat 845.998 kb on + strand, within glnPat 846.069 kb on + strand, within glnPat 846.069 kb on + strand, within glnPat 846.069 kb on + strand, within glnPat 846.085 kb on + strand, within glnPat 846.154 kb on - strand, within glnPat 846.209 kb on - strand, within glnPat 846.326 kb on + strandat 846.326 kb on + strandat 846.420 kb on + strandat 846.420 kb on + strandat 846.434 kb on + strandat 846.434 kb on + strandat 846.502 kb on - strandat 846.583 kb on + strand, within glnHat 846.583 kb on + strand, within glnHat 846.604 kb on + strand, within glnHat 846.658 kb on - strand, within glnHat 846.694 kb on + strand, within glnHat 846.727 kb on - strand, within glnHat 846.729 kb on + strand, within glnHat 846.731 kb on + strand, within glnHat 846.734 kb on - strand, within glnHat 846.745 kb on - strand, within glnHat 846.745 kb on - strand, within glnHat 846.812 kb on - strand, within glnHat 846.817 kb on + strand, within glnHat 846.817 kb on + strand, within glnHat 847.125 kb on + strand, within glnHat 847.125 kb on + strand, within glnHat 847.218 kb on - strandat 847.218 kb on - strandat 847.222 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ciprofloxacin Hydrochloride 0.0024 mM
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844,694 - +2.6
844,712 - +0.1
845,109 + glnQ b0809 0.20 -0.3
845,154 + glnQ b0809 0.26 -3.0
845,243 + glnQ b0809 0.39 -1.7
845,243 + glnQ b0809 0.39 +0.2
845,288 + glnQ b0809 0.45 +0.6
845,547 - glnQ b0809 0.81 +0.3
845,569 + glnQ b0809 0.84 -1.1
845,596 - glnQ b0809 0.87 -0.4
845,596 - glnQ b0809 0.87 -2.8
845,994 + glnP b0810 0.47 -1.7
845,994 + glnP b0810 0.47 -1.8
845,997 - glnP b0810 0.48 -0.2
845,998 + glnP b0810 0.48 -0.0
845,998 + glnP b0810 0.48 -0.2
846,069 + glnP b0810 0.58 -2.0
846,069 + glnP b0810 0.58 -1.3
846,069 + glnP b0810 0.58 -0.3
846,085 + glnP b0810 0.61 -1.8
846,154 - glnP b0810 0.71 -0.2
846,209 - glnP b0810 0.80 -1.7
846,326 + -0.4
846,326 + +0.0
846,420 + -0.5
846,420 + -1.7
846,434 + -0.6
846,434 + +0.1
846,502 - -1.9
846,583 + glnH b0811 0.14 +1.7
846,583 + glnH b0811 0.14 +2.7
846,604 + glnH b0811 0.16 +0.0
846,658 - glnH b0811 0.24 +0.8
846,694 + glnH b0811 0.29 +1.3
846,727 - glnH b0811 0.33 +0.3
846,729 + glnH b0811 0.33 -2.3
846,731 + glnH b0811 0.33 -0.9
846,734 - glnH b0811 0.34 -0.5
846,745 - glnH b0811 0.35 -3.5
846,745 - glnH b0811 0.35 -0.3
846,812 - glnH b0811 0.44 -3.4
846,817 + glnH b0811 0.45 -1.1
846,817 + glnH b0811 0.45 -0.9
847,125 + glnH b0811 0.86 +1.3
847,125 + glnH b0811 0.86 -0.2
847,218 - +0.9
847,218 - -0.0
847,222 + +0.6

Or see this region's nucleotide sequence