Experiment: Ciprofloxacin Hydrochloride 0.0024 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fiu and ybiM are separated by 264 nucleotides ybiM and ybiN are separated by 275 nucleotides ybiN and ybiO overlap by 4 nucleotides
b0805: fiu - predicted iron outer membrane transporter (NCBI), at 838,472 to 840,754
fiu
b0806: ybiM - orf, hypothetical protein (VIMSS), at 841,019 to 841,279
ybiM
b0807: ybiN - orf, hypothetical protein (VIMSS), at 841,555 to 842,481
ybiN
b0808: ybiO - putative transport protein (VIMSS), at 842,478 to 844,703
ybiO
Position (kb) 841
842
843 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 840.820 kb on + strand at 840.832 kb on - strand at 840.832 kb on - strand at 841.192 kb on + strand, within ybiM at 841.223 kb on - strand, within ybiM at 841.327 kb on + strand at 841.327 kb on + strand at 841.546 kb on - strand at 841.559 kb on - strand at 841.559 kb on - strand at 841.559 kb on - strand at 841.658 kb on + strand, within ybiN at 841.678 kb on - strand, within ybiN at 841.682 kb on + strand, within ybiN at 841.689 kb on - strand, within ybiN at 841.769 kb on - strand, within ybiN at 841.792 kb on + strand, within ybiN at 841.976 kb on + strand, within ybiN at 841.979 kb on - strand, within ybiN at 841.986 kb on + strand, within ybiN at 842.073 kb on - strand, within ybiN at 842.220 kb on - strand, within ybiN at 842.461 kb on + strand at 842.519 kb on - strand at 842.601 kb on - strand at 842.671 kb on + strand at 842.941 kb on - strand, within ybiO at 842.962 kb on - strand, within ybiO at 843.218 kb on - strand, within ybiO at 843.370 kb on - strand, within ybiO
Per-strain Table
Position Strand Gene LocusTag Fraction Ciprofloxacin Hydrochloride 0.0024 mM remove 840,820 + -3.4 840,832 - -0.7 840,832 - +0.6 841,192 + ybiM b0806 0.66 +0.3 841,223 - ybiM b0806 0.78 +0.1 841,327 + -0.0 841,327 + -1.0 841,546 - -0.4 841,559 - +0.9 841,559 - -0.5 841,559 - -1.6 841,658 + ybiN b0807 0.11 -0.9 841,678 - ybiN b0807 0.13 -0.2 841,682 + ybiN b0807 0.14 +0.6 841,689 - ybiN b0807 0.14 -2.1 841,769 - ybiN b0807 0.23 +0.4 841,792 + ybiN b0807 0.26 +1.3 841,976 + ybiN b0807 0.45 -0.3 841,979 - ybiN b0807 0.46 +2.3 841,986 + ybiN b0807 0.46 +0.3 842,073 - ybiN b0807 0.56 +0.6 842,220 - ybiN b0807 0.72 +0.8 842,461 + +0.5 842,519 - -0.3 842,601 - -2.3 842,671 + +1.0 842,941 - ybiO b0808 0.21 -0.4 842,962 - ybiO b0808 0.22 -0.0 843,218 - ybiO b0808 0.33 +0.2 843,370 - ybiO b0808 0.40 +2.3
Or see this region's nucleotide sequence