Strain Fitness in Escherichia coli BW25113 around b2583

Experiment: Irgasan 0.0024 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfiF and trxC are separated by 206 nucleotidestrxC and yfiP are separated by 68 nucleotidesyfiP and yfiQ are separated by 31 nucleotides b2581: yfiF - predicted methyltransferase (NCBI), at 2,715,513 to 2,716,550 yfiF b2582: trxC - thioredoxin 2 (NCBI), at 2,716,757 to 2,717,176 trxC b2583: yfiP - orf, hypothetical protein (VIMSS), at 2,717,245 to 2,717,943 yfiP b2584: yfiQ - fused predicted acyl-CoA synthetase: NAD(P)-binding subunit/ATP-binding subunit (NCBI), at 2,717,975 to 2,720,635 yfiQ Position (kb) 2717 2718Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5 6 7at 2716.265 kb on - strand, within yfiFat 2716.265 kb on - strand, within yfiFat 2716.313 kb on + strand, within yfiFat 2716.313 kb on + strand, within yfiFat 2716.387 kb on - strand, within yfiFat 2716.387 kb on - strand, within yfiFat 2716.440 kb on + strand, within yfiFat 2716.446 kb on + strand, within yfiFat 2716.485 kb on + strandat 2716.742 kb on + strandat 2716.762 kb on - strandat 2716.832 kb on + strand, within trxCat 2717.016 kb on - strand, within trxCat 2717.103 kb on + strand, within trxCat 2717.387 kb on - strand, within yfiPat 2717.387 kb on - strand, within yfiPat 2717.445 kb on + strand, within yfiPat 2717.445 kb on + strand, within yfiPat 2717.458 kb on - strand, within yfiPat 2717.591 kb on - strand, within yfiPat 2717.591 kb on - strand, within yfiPat 2717.605 kb on + strand, within yfiPat 2717.612 kb on + strand, within yfiPat 2717.612 kb on + strand, within yfiPat 2717.767 kb on - strand, within yfiPat 2717.817 kb on - strand, within yfiPat 2717.846 kb on + strand, within yfiPat 2717.846 kb on + strand, within yfiPat 2717.854 kb on - strand, within yfiPat 2717.902 kb on + strandat 2717.902 kb on + strandat 2717.979 kb on - strandat 2718.064 kb on - strandat 2718.151 kb on - strandat 2718.151 kb on - strandat 2718.309 kb on + strand, within yfiQat 2718.337 kb on - strand, within yfiQat 2718.461 kb on + strand, within yfiQat 2718.469 kb on - strand, within yfiQat 2718.471 kb on + strand, within yfiQat 2718.481 kb on + strand, within yfiQat 2718.534 kb on + strand, within yfiQat 2718.534 kb on + strand, within yfiQat 2718.597 kb on - strand, within yfiQat 2718.681 kb on + strand, within yfiQat 2718.773 kb on + strand, within yfiQ

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Per-strain Table

Position Strand Gene LocusTag Fraction Irgasan 0.0024 mM
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2,716,265 - yfiF b2581 0.72 -0.3
2,716,265 - yfiF b2581 0.72 -2.8
2,716,313 + yfiF b2581 0.77 +0.1
2,716,313 + yfiF b2581 0.77 -0.7
2,716,387 - yfiF b2581 0.84 +0.8
2,716,387 - yfiF b2581 0.84 +0.6
2,716,440 + yfiF b2581 0.89 -0.2
2,716,446 + yfiF b2581 0.90 -1.0
2,716,485 + -1.0
2,716,742 + -0.1
2,716,762 - -0.2
2,716,832 + trxC b2582 0.18 -0.2
2,717,016 - trxC b2582 0.62 +0.8
2,717,103 + trxC b2582 0.82 -0.2
2,717,387 - yfiP b2583 0.20 +0.4
2,717,387 - yfiP b2583 0.20 -1.6
2,717,445 + yfiP b2583 0.29 -0.6
2,717,445 + yfiP b2583 0.29 +0.0
2,717,458 - yfiP b2583 0.30 -0.2
2,717,591 - yfiP b2583 0.49 -0.5
2,717,591 - yfiP b2583 0.49 +7.2
2,717,605 + yfiP b2583 0.52 +0.7
2,717,612 + yfiP b2583 0.53 -0.2
2,717,612 + yfiP b2583 0.53 -0.7
2,717,767 - yfiP b2583 0.75 -0.3
2,717,817 - yfiP b2583 0.82 +0.2
2,717,846 + yfiP b2583 0.86 -0.6
2,717,846 + yfiP b2583 0.86 +0.8
2,717,854 - yfiP b2583 0.87 -0.0
2,717,902 + +0.2
2,717,902 + +0.3
2,717,979 - +0.7
2,718,064 - -0.3
2,718,151 - +0.1
2,718,151 - +0.1
2,718,309 + yfiQ b2584 0.13 -0.2
2,718,337 - yfiQ b2584 0.14 -0.2
2,718,461 + yfiQ b2584 0.18 -0.1
2,718,469 - yfiQ b2584 0.19 +0.4
2,718,471 + yfiQ b2584 0.19 +0.2
2,718,481 + yfiQ b2584 0.19 -0.2
2,718,534 + yfiQ b2584 0.21 +0.3
2,718,534 + yfiQ b2584 0.21 +0.9
2,718,597 - yfiQ b2584 0.23 +0.5
2,718,681 + yfiQ b2584 0.27 -0.0
2,718,773 + yfiQ b2584 0.30 +1.3

Or see this region's nucleotide sequence