Experiment: Trimethoprim 0.005 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yehY and yehZ are separated by 6 nucleotides yehZ and bglX are separated by 210 nucleotides
b2130: yehY - predicted transporter subunit: membrane component of ABC superfamily (NCBI), at 2,215,422 to 2,216,579
yehY
b2131: yehZ - predicted transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 2,216,586 to 2,217,503
yehZ
b2132: bglX - beta-D-glucoside glucohydrolase, periplasmic (NCBI), at 2,217,714 to 2,220,011
bglX
Position (kb)
2216
2217
2218 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2215.683 kb on - strand, within yehY at 2215.683 kb on - strand, within yehY at 2215.698 kb on - strand, within yehY at 2215.698 kb on - strand, within yehY at 2215.701 kb on - strand, within yehY at 2215.805 kb on + strand, within yehY at 2215.876 kb on - strand, within yehY at 2215.909 kb on - strand, within yehY at 2215.909 kb on - strand, within yehY at 2215.998 kb on + strand, within yehY at 2216.025 kb on + strand, within yehY at 2216.025 kb on + strand, within yehY at 2216.209 kb on + strand, within yehY at 2216.209 kb on + strand, within yehY at 2216.217 kb on - strand, within yehY at 2216.217 kb on - strand, within yehY at 2216.355 kb on - strand, within yehY at 2216.544 kb on - strand at 2216.627 kb on + strand at 2216.745 kb on - strand, within yehZ at 2216.745 kb on - strand, within yehZ at 2216.748 kb on - strand, within yehZ at 2216.767 kb on - strand, within yehZ at 2216.782 kb on + strand, within yehZ at 2216.950 kb on - strand, within yehZ at 2216.950 kb on - strand, within yehZ at 2216.955 kb on + strand, within yehZ at 2216.955 kb on + strand, within yehZ at 2217.019 kb on + strand, within yehZ at 2217.153 kb on - strand, within yehZ at 2217.153 kb on - strand, within yehZ at 2217.260 kb on + strand, within yehZ at 2217.311 kb on - strand, within yehZ at 2217.311 kb on - strand, within yehZ at 2217.328 kb on + strand, within yehZ at 2217.376 kb on + strand, within yehZ at 2217.463 kb on + strand at 2217.470 kb on + strand at 2217.560 kb on - strand at 2217.621 kb on - strand at 2217.682 kb on - strand at 2217.769 kb on + strand at 2218.066 kb on + strand, within bglX at 2218.089 kb on + strand, within bglX at 2218.089 kb on + strand, within bglX at 2218.305 kb on - strand, within bglX at 2218.470 kb on + strand, within bglX at 2218.470 kb on + strand, within bglX at 2218.476 kb on - strand, within bglX at 2218.476 kb on - strand, within bglX at 2218.483 kb on - strand, within bglX at 2218.483 kb on - strand, within bglX
Per-strain Table
Position Strand Gene LocusTag Fraction Trimethoprim 0.005 mM remove 2,215,683 - yehY b2130 0.23 -0.4 2,215,683 - yehY b2130 0.23 -0.9 2,215,698 - yehY b2130 0.24 -0.8 2,215,698 - yehY b2130 0.24 -2.0 2,215,701 - yehY b2130 0.24 -0.6 2,215,805 + yehY b2130 0.33 -0.7 2,215,876 - yehY b2130 0.39 -0.6 2,215,909 - yehY b2130 0.42 -2.1 2,215,909 - yehY b2130 0.42 -0.4 2,215,998 + yehY b2130 0.50 +2.7 2,216,025 + yehY b2130 0.52 -0.8 2,216,025 + yehY b2130 0.52 -1.2 2,216,209 + yehY b2130 0.68 +1.2 2,216,209 + yehY b2130 0.68 -1.2 2,216,217 - yehY b2130 0.69 -0.3 2,216,217 - yehY b2130 0.69 +1.3 2,216,355 - yehY b2130 0.81 -0.8 2,216,544 - -0.6 2,216,627 + -1.6 2,216,745 - yehZ b2131 0.17 -1.3 2,216,745 - yehZ b2131 0.17 +4.3 2,216,748 - yehZ b2131 0.18 -1.7 2,216,767 - yehZ b2131 0.20 -2.0 2,216,782 + yehZ b2131 0.21 -1.3 2,216,950 - yehZ b2131 0.40 +0.7 2,216,950 - yehZ b2131 0.40 +0.1 2,216,955 + yehZ b2131 0.40 +0.6 2,216,955 + yehZ b2131 0.40 -1.1 2,217,019 + yehZ b2131 0.47 -0.3 2,217,153 - yehZ b2131 0.62 +1.6 2,217,153 - yehZ b2131 0.62 -0.3 2,217,260 + yehZ b2131 0.73 -1.4 2,217,311 - yehZ b2131 0.79 -0.7 2,217,311 - yehZ b2131 0.79 +0.1 2,217,328 + yehZ b2131 0.81 -0.6 2,217,376 + yehZ b2131 0.86 -1.8 2,217,463 + -0.8 2,217,470 + -0.7 2,217,560 - +1.3 2,217,621 - -1.6 2,217,682 - -0.7 2,217,769 + -2.9 2,218,066 + bglX b2132 0.15 +0.8 2,218,089 + bglX b2132 0.16 -0.9 2,218,089 + bglX b2132 0.16 -0.7 2,218,305 - bglX b2132 0.26 -1.3 2,218,470 + bglX b2132 0.33 -2.0 2,218,470 + bglX b2132 0.33 +1.4 2,218,476 - bglX b2132 0.33 -1.0 2,218,476 - bglX b2132 0.33 -0.4 2,218,483 - bglX b2132 0.33 -0.5 2,218,483 - bglX b2132 0.33 -1.6
Or see this region's nucleotide sequence