Strain Fitness in Escherichia coli BW25113 around b1798

Experiment: Trimethoprim 0.005 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyeaQ and yoaG are separated by 147 nucleotidesyoaG and yeaR are separated by 3 nucleotidesyeaR and yeaS are separated by 172 nucleotidesyeaS and yeaT are separated by 126 nucleotides b1795: yeaQ - conserved inner membrane protein (NCBI), at 1,877,031 to 1,877,279 yeaQ b1796: yoaG - hypothetical protein (NCBI), at 1,877,427 to 1,877,609 yoaG b1797: yeaR - hypothetical protein (NCBI), at 1,877,613 to 1,877,972 yeaR b1798: yeaS - neutral amino-acid efflux system (NCBI), at 1,878,145 to 1,878,783 yeaS b1799: yeaT - putative transcriptional regulator LYSR-type (VIMSS), at 1,878,910 to 1,879,833 yeaT Position (kb) 1878 1879Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1877.145 kb on - strand, within yeaQat 1877.145 kb on - strand, within yeaQat 1877.174 kb on - strand, within yeaQat 1877.175 kb on - strand, within yeaQat 1877.260 kb on + strandat 1877.433 kb on + strandat 1877.476 kb on + strand, within yoaGat 1877.476 kb on + strand, within yoaGat 1877.484 kb on - strand, within yoaGat 1877.513 kb on - strand, within yoaGat 1877.571 kb on - strand, within yoaGat 1877.602 kb on - strandat 1877.658 kb on - strand, within yeaRat 1877.663 kb on + strand, within yeaRat 1877.663 kb on + strand, within yeaRat 1877.714 kb on + strand, within yeaRat 1877.715 kb on - strand, within yeaRat 1877.764 kb on + strand, within yeaRat 1877.765 kb on + strand, within yeaRat 1877.765 kb on + strand, within yeaRat 1877.791 kb on + strand, within yeaRat 1877.804 kb on - strand, within yeaRat 1877.804 kb on - strand, within yeaRat 1877.804 kb on - strand, within yeaRat 1877.814 kb on - strand, within yeaRat 1877.859 kb on - strand, within yeaRat 1877.985 kb on + strandat 1878.018 kb on - strandat 1878.085 kb on - strandat 1878.142 kb on - strandat 1878.142 kb on - strandat 1878.186 kb on + strandat 1878.236 kb on + strand, within yeaSat 1878.236 kb on + strand, within yeaSat 1878.236 kb on + strand, within yeaSat 1878.236 kb on + strand, within yeaSat 1878.244 kb on - strand, within yeaSat 1878.244 kb on - strand, within yeaSat 1878.244 kb on - strand, within yeaSat 1878.244 kb on - strand, within yeaSat 1878.303 kb on - strand, within yeaSat 1878.340 kb on + strand, within yeaSat 1878.363 kb on + strand, within yeaSat 1878.363 kb on + strand, within yeaSat 1878.364 kb on - strand, within yeaSat 1878.388 kb on - strand, within yeaSat 1878.414 kb on - strand, within yeaSat 1878.446 kb on + strand, within yeaSat 1878.454 kb on - strand, within yeaSat 1878.484 kb on + strand, within yeaSat 1878.484 kb on + strand, within yeaSat 1878.492 kb on - strand, within yeaSat 1878.492 kb on - strand, within yeaSat 1878.492 kb on - strand, within yeaSat 1878.511 kb on + strand, within yeaSat 1878.598 kb on - strand, within yeaSat 1878.598 kb on - strand, within yeaSat 1878.598 kb on - strand, within yeaSat 1878.598 kb on - strand, within yeaSat 1878.608 kb on - strand, within yeaSat 1878.656 kb on - strand, within yeaSat 1878.664 kb on - strand, within yeaSat 1878.721 kb on - strandat 1878.738 kb on + strandat 1878.746 kb on - strandat 1878.752 kb on - strandat 1878.757 kb on + strandat 1878.757 kb on + strandat 1878.775 kb on + strandat 1878.841 kb on + strandat 1878.940 kb on + strandat 1879.004 kb on - strand, within yeaTat 1879.009 kb on + strand, within yeaTat 1879.027 kb on - strand, within yeaTat 1879.118 kb on - strand, within yeaTat 1879.175 kb on - strand, within yeaTat 1879.175 kb on - strand, within yeaTat 1879.210 kb on - strand, within yeaTat 1879.210 kb on - strand, within yeaTat 1879.231 kb on - strand, within yeaTat 1879.245 kb on + strand, within yeaTat 1879.253 kb on - strand, within yeaTat 1879.266 kb on + strand, within yeaTat 1879.340 kb on - strand, within yeaTat 1879.340 kb on - strand, within yeaTat 1879.410 kb on + strand, within yeaTat 1879.426 kb on + strand, within yeaTat 1879.601 kb on - strand, within yeaT

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Per-strain Table

Position Strand Gene LocusTag Fraction Trimethoprim 0.005 mM
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1,877,145 - yeaQ b1795 0.46 -0.3
1,877,145 - yeaQ b1795 0.46 -1.6
1,877,174 - yeaQ b1795 0.57 +1.0
1,877,175 - yeaQ b1795 0.58 -0.3
1,877,260 + +0.7
1,877,433 + -1.1
1,877,476 + yoaG b1796 0.27 -1.4
1,877,476 + yoaG b1796 0.27 -0.8
1,877,484 - yoaG b1796 0.31 -0.6
1,877,513 - yoaG b1796 0.47 -0.3
1,877,571 - yoaG b1796 0.79 +0.2
1,877,602 - -0.0
1,877,658 - yeaR b1797 0.12 -1.1
1,877,663 + yeaR b1797 0.14 -0.4
1,877,663 + yeaR b1797 0.14 -1.1
1,877,714 + yeaR b1797 0.28 -1.3
1,877,715 - yeaR b1797 0.28 +0.4
1,877,764 + yeaR b1797 0.42 -1.0
1,877,765 + yeaR b1797 0.42 -0.7
1,877,765 + yeaR b1797 0.42 -0.3
1,877,791 + yeaR b1797 0.49 +1.3
1,877,804 - yeaR b1797 0.53 -0.4
1,877,804 - yeaR b1797 0.53 +3.3
1,877,804 - yeaR b1797 0.53 -1.9
1,877,814 - yeaR b1797 0.56 +0.7
1,877,859 - yeaR b1797 0.68 -1.7
1,877,985 + -0.7
1,878,018 - +0.4
1,878,085 - +0.7
1,878,142 - +0.1
1,878,142 - -1.4
1,878,186 + +2.3
1,878,236 + yeaS b1798 0.14 +0.7
1,878,236 + yeaS b1798 0.14 +2.3
1,878,236 + yeaS b1798 0.14 +2.3
1,878,236 + yeaS b1798 0.14 +1.3
1,878,244 - yeaS b1798 0.15 +1.3
1,878,244 - yeaS b1798 0.15 -1.3
1,878,244 - yeaS b1798 0.15 +1.9
1,878,244 - yeaS b1798 0.15 +3.3
1,878,303 - yeaS b1798 0.25 -0.8
1,878,340 + yeaS b1798 0.31 +0.5
1,878,363 + yeaS b1798 0.34 +1.7
1,878,363 + yeaS b1798 0.34 +1.3
1,878,364 - yeaS b1798 0.34 +3.4
1,878,388 - yeaS b1798 0.38 -0.7
1,878,414 - yeaS b1798 0.42 +0.2
1,878,446 + yeaS b1798 0.47 +2.6
1,878,454 - yeaS b1798 0.48 -0.8
1,878,484 + yeaS b1798 0.53 +3.3
1,878,484 + yeaS b1798 0.53 +2.3
1,878,492 - yeaS b1798 0.54 -0.7
1,878,492 - yeaS b1798 0.54 +1.7
1,878,492 - yeaS b1798 0.54 +1.2
1,878,511 + yeaS b1798 0.57 +3.3
1,878,598 - yeaS b1798 0.71 +1.0
1,878,598 - yeaS b1798 0.71 -1.8
1,878,598 - yeaS b1798 0.71 -0.4
1,878,598 - yeaS b1798 0.71 +1.4
1,878,608 - yeaS b1798 0.72 +1.2
1,878,656 - yeaS b1798 0.80 +0.7
1,878,664 - yeaS b1798 0.81 -0.1
1,878,721 - +0.7
1,878,738 + +3.3
1,878,746 - -0.0
1,878,752 - +1.5
1,878,757 + +3.3
1,878,757 + +3.3
1,878,775 + +3.3
1,878,841 + +3.3
1,878,940 + +3.3
1,879,004 - yeaT b1799 0.10 -0.2
1,879,009 + yeaT b1799 0.11 +3.3
1,879,027 - yeaT b1799 0.13 -0.7
1,879,118 - yeaT b1799 0.23 +1.0
1,879,175 - yeaT b1799 0.29 -0.6
1,879,175 - yeaT b1799 0.29 -2.4
1,879,210 - yeaT b1799 0.32 +0.2
1,879,210 - yeaT b1799 0.32 +0.7
1,879,231 - yeaT b1799 0.35 -0.3
1,879,245 + yeaT b1799 0.36 +3.3
1,879,253 - yeaT b1799 0.37 +2.3
1,879,266 + yeaT b1799 0.39 +0.9
1,879,340 - yeaT b1799 0.47 +1.0
1,879,340 - yeaT b1799 0.47 +0.5
1,879,410 + yeaT b1799 0.54 +2.3
1,879,426 + yeaT b1799 0.56 +2.3
1,879,601 - yeaT b1799 0.75 +0.3

Or see this region's nucleotide sequence