Experiment: Trimethoprim 0.005 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pps and ydiA are separated by 332 nucleotides ydiA and aroH are separated by 156 nucleotides
b1702: pps - phosphoenolpyruvate synthase (NCBI), at 1,782,758 to 1,785,136
pps
b1703: ydiA - hypothetical protein (NCBI), at 1,785,469 to 1,786,302
ydiA
b1704: aroH - 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, tryptophan repressible (NCBI), at 1,786,459 to 1,787,505
aroH
Position (kb)
1785
1786
1787 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1784.472 kb on - strand, within pps at 1784.598 kb on + strand, within pps at 1784.610 kb on + strand, within pps at 1784.832 kb on + strand, within pps at 1784.832 kb on + strand, within pps at 1784.954 kb on + strand at 1785.015 kb on + strand at 1785.056 kb on + strand at 1785.076 kb on + strand at 1785.101 kb on + strand at 1785.101 kb on + strand at 1785.111 kb on + strand at 1785.268 kb on + strand at 1785.268 kb on + strand at 1785.399 kb on - strand at 1785.739 kb on + strand, within ydiA at 1785.765 kb on + strand, within ydiA at 1785.814 kb on - strand, within ydiA at 1785.826 kb on - strand, within ydiA at 1785.827 kb on - strand, within ydiA at 1785.827 kb on - strand, within ydiA at 1785.918 kb on + strand, within ydiA at 1785.918 kb on + strand, within ydiA at 1786.154 kb on + strand, within ydiA at 1786.164 kb on + strand, within ydiA at 1786.192 kb on + strand, within ydiA at 1786.224 kb on + strand at 1786.412 kb on + strand at 1786.464 kb on - strand at 1786.530 kb on - strand at 1786.532 kb on + strand at 1786.532 kb on + strand at 1786.543 kb on - strand at 1786.543 kb on - strand at 1786.553 kb on - strand at 1786.553 kb on - strand at 1786.632 kb on + strand, within aroH at 1786.668 kb on - strand, within aroH at 1786.668 kb on - strand, within aroH at 1786.722 kb on + strand, within aroH at 1786.897 kb on - strand, within aroH at 1787.055 kb on - strand, within aroH at 1787.210 kb on - strand, within aroH at 1787.217 kb on - strand, within aroH
Per-strain Table
Position Strand Gene LocusTag Fraction Trimethoprim 0.005 mM remove 1,784,472 - pps b1702 0.72 -2.2 1,784,598 + pps b1702 0.77 -0.4 1,784,610 + pps b1702 0.78 -0.5 1,784,832 + pps b1702 0.87 -0.2 1,784,832 + pps b1702 0.87 +0.3 1,784,954 + +0.4 1,785,015 + +1.5 1,785,056 + -0.2 1,785,076 + -1.8 1,785,101 + +3.5 1,785,101 + +2.5 1,785,111 + +0.3 1,785,268 + +2.5 1,785,268 + +0.2 1,785,399 - +2.5 1,785,739 + ydiA b1703 0.32 -1.0 1,785,765 + ydiA b1703 0.35 -1.6 1,785,814 - ydiA b1703 0.41 -1.4 1,785,826 - ydiA b1703 0.43 +1.5 1,785,827 - ydiA b1703 0.43 -0.2 1,785,827 - ydiA b1703 0.43 -0.1 1,785,918 + ydiA b1703 0.54 -1.4 1,785,918 + ydiA b1703 0.54 +0.7 1,786,154 + ydiA b1703 0.82 -1.5 1,786,164 + ydiA b1703 0.83 -1.0 1,786,192 + ydiA b1703 0.87 -0.9 1,786,224 + +0.6 1,786,412 + -0.6 1,786,464 - +0.9 1,786,530 - +1.6 1,786,532 + -1.3 1,786,532 + -0.3 1,786,543 - +0.4 1,786,543 - -0.8 1,786,553 - -0.8 1,786,553 - -0.1 1,786,632 + aroH b1704 0.17 -1.3 1,786,668 - aroH b1704 0.20 -0.2 1,786,668 - aroH b1704 0.20 -0.4 1,786,722 + aroH b1704 0.25 -1.1 1,786,897 - aroH b1704 0.42 -1.0 1,787,055 - aroH b1704 0.57 -0.8 1,787,210 - aroH b1704 0.72 -0.4 1,787,217 - aroH b1704 0.72 -0.5
Or see this region's nucleotide sequence