Experiment: KB with Harmine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1743 and Psyr_RNA31 are separated by 296 nucleotides Psyr_RNA31 and Psyr_RNA32 are separated by 51 nucleotides Psyr_RNA32 and Psyr_RNA33 are separated by 56 nucleotides Psyr_RNA33 and Psyr_1744 are separated by 189 nucleotides Psyr_1744 and Psyr_1745 are separated by 85 nucleotides
Psyr_1743: Psyr_1743 - methenyltetrahydrofolate cyclohydrolase / 5,10-methylenetetrahydrofolate dehydrogenase (NADP+), at 1,980,210 to 1,981,064
_1743
Psyr_RNA31: Psyr_RNA31 - tRNA-Arg, at 1,981,361 to 1,981,437
_RNA31
Psyr_RNA32: Psyr_RNA32 - tRNA-His, at 1,981,489 to 1,981,564
_RNA32
Psyr_RNA33: Psyr_RNA33 - tRNA-Leu, at 1,981,621 to 1,981,705
_RNA33
Psyr_1744: Psyr_1744 - lipoprotein, putative, at 1,981,895 to 1,982,386
_1744
Psyr_1745: Psyr_1745 - hypothetical protein, at 1,982,472 to 1,983,302
_1745
Position (kb)
1981
1982 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1981.051 kb on - strand at 1981.157 kb on + strand at 1981.167 kb on - strand at 1981.568 kb on + strand at 1981.568 kb on + strand at 1981.568 kb on + strand at 1981.568 kb on + strand at 1981.568 kb on + strand at 1981.588 kb on + strand at 1981.710 kb on + strand at 1981.788 kb on + strand at 1981.791 kb on + strand at 1981.791 kb on + strand at 1981.792 kb on - strand at 1981.792 kb on - strand at 1981.913 kb on + strand at 1981.913 kb on + strand at 1981.913 kb on + strand at 1981.913 kb on + strand at 1981.914 kb on - strand at 1981.995 kb on - strand, within Psyr_1744 at 1982.041 kb on + strand, within Psyr_1744 at 1982.042 kb on - strand, within Psyr_1744 at 1982.043 kb on + strand, within Psyr_1744 at 1982.043 kb on + strand, within Psyr_1744 at 1982.043 kb on + strand, within Psyr_1744 at 1982.043 kb on + strand, within Psyr_1744 at 1982.043 kb on + strand, within Psyr_1744 at 1982.044 kb on - strand, within Psyr_1744 at 1982.044 kb on - strand, within Psyr_1744 at 1982.044 kb on - strand, within Psyr_1744 at 1982.109 kb on + strand, within Psyr_1744 at 1982.202 kb on + strand, within Psyr_1744 at 1982.202 kb on + strand, within Psyr_1744 at 1982.202 kb on + strand, within Psyr_1744 at 1982.202 kb on + strand, within Psyr_1744 at 1982.203 kb on - strand, within Psyr_1744 at 1982.203 kb on - strand, within Psyr_1744 at 1982.203 kb on - strand, within Psyr_1744 at 1982.203 kb on - strand, within Psyr_1744 at 1982.203 kb on - strand, within Psyr_1744 at 1982.203 kb on - strand, within Psyr_1744 at 1982.203 kb on - strand, within Psyr_1744 at 1982.203 kb on - strand, within Psyr_1744 at 1982.203 kb on - strand, within Psyr_1744 at 1982.251 kb on + strand, within Psyr_1744 at 1982.251 kb on + strand, within Psyr_1744 at 1982.252 kb on - strand, within Psyr_1744 at 1982.274 kb on + strand, within Psyr_1744 at 1982.274 kb on + strand, within Psyr_1744 at 1982.274 kb on + strand, within Psyr_1744 at 1982.274 kb on + strand, within Psyr_1744 at 1982.275 kb on - strand, within Psyr_1744 at 1982.275 kb on - strand, within Psyr_1744 at 1982.275 kb on - strand, within Psyr_1744 at 1982.382 kb on + strand at 1982.382 kb on + strand at 1982.382 kb on + strand at 1982.383 kb on - strand at 1982.413 kb on + strand at 1982.413 kb on + strand at 1982.414 kb on - strand at 1982.438 kb on - strand at 1982.446 kb on + strand at 1982.529 kb on + strand at 1982.530 kb on - strand at 1982.530 kb on - strand at 1982.543 kb on + strand at 1982.544 kb on - strand at 1982.544 kb on - strand at 1982.551 kb on + strand at 1982.551 kb on + strand at 1982.551 kb on + strand at 1982.552 kb on - strand at 1982.552 kb on - strand at 1982.552 kb on - strand at 1982.552 kb on - strand at 1982.673 kb on - strand, within Psyr_1745 at 1982.673 kb on - strand, within Psyr_1745 at 1982.694 kb on - strand, within Psyr_1745
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmine 10 mM remove 1,981,051 - -0.5 1,981,157 + -0.2 1,981,167 - -0.1 1,981,568 + +0.5 1,981,568 + +1.3 1,981,568 + +0.1 1,981,568 + -0.5 1,981,568 + +1.8 1,981,588 + +1.6 1,981,710 + -0.9 1,981,788 + +1.0 1,981,791 + +0.5 1,981,791 + -0.5 1,981,792 - -1.1 1,981,792 - +1.1 1,981,913 + -1.3 1,981,913 + -0.4 1,981,913 + +0.0 1,981,913 + -2.0 1,981,914 - +2.3 1,981,995 - Psyr_1744 0.20 +0.8 1,982,041 + Psyr_1744 0.30 -0.2 1,982,042 - Psyr_1744 0.30 +0.6 1,982,043 + Psyr_1744 0.30 -0.3 1,982,043 + Psyr_1744 0.30 -0.2 1,982,043 + Psyr_1744 0.30 +0.1 1,982,043 + Psyr_1744 0.30 -0.0 1,982,043 + Psyr_1744 0.30 -0.1 1,982,044 - Psyr_1744 0.30 +1.7 1,982,044 - Psyr_1744 0.30 +0.1 1,982,044 - Psyr_1744 0.30 +0.1 1,982,109 + Psyr_1744 0.43 -1.5 1,982,202 + Psyr_1744 0.62 -1.4 1,982,202 + Psyr_1744 0.62 -1.3 1,982,202 + Psyr_1744 0.62 +0.1 1,982,202 + Psyr_1744 0.62 +0.1 1,982,203 - Psyr_1744 0.63 +0.9 1,982,203 - Psyr_1744 0.63 -0.9 1,982,203 - Psyr_1744 0.63 -2.1 1,982,203 - Psyr_1744 0.63 -2.2 1,982,203 - Psyr_1744 0.63 +0.1 1,982,203 - Psyr_1744 0.63 -0.1 1,982,203 - Psyr_1744 0.63 -0.7 1,982,203 - Psyr_1744 0.63 +1.3 1,982,203 - Psyr_1744 0.63 -0.0 1,982,251 + Psyr_1744 0.72 +0.1 1,982,251 + Psyr_1744 0.72 -1.8 1,982,252 - Psyr_1744 0.73 -2.2 1,982,274 + Psyr_1744 0.77 -0.4 1,982,274 + Psyr_1744 0.77 -0.7 1,982,274 + Psyr_1744 0.77 -1.7 1,982,274 + Psyr_1744 0.77 +2.0 1,982,275 - Psyr_1744 0.77 -0.3 1,982,275 - Psyr_1744 0.77 +0.5 1,982,275 - Psyr_1744 0.77 +0.3 1,982,382 + -0.4 1,982,382 + +1.7 1,982,382 + -0.3 1,982,383 - -0.6 1,982,413 + +0.5 1,982,413 + +1.0 1,982,414 - +0.2 1,982,438 - -1.6 1,982,446 + -0.9 1,982,529 + -0.8 1,982,530 - -0.0 1,982,530 - +2.0 1,982,543 + -0.5 1,982,544 - -0.5 1,982,544 - -1.5 1,982,551 + -0.1 1,982,551 + -0.0 1,982,551 + +0.2 1,982,552 - -0.7 1,982,552 - +0.5 1,982,552 - +1.5 1,982,552 - -0.1 1,982,673 - Psyr_1745 0.24 +0.1 1,982,673 - Psyr_1745 0.24 -0.4 1,982,694 - Psyr_1745 0.27 +1.1
Or see this region's nucleotide sequence