Experiment: KB with Harmine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4153 and Psyr_4154 are separated by 988 nucleotides Psyr_4154 and Psyr_4155 are separated by 107 nucleotides Psyr_4155 and Psyr_4156 are separated by 48 nucleotides
Psyr_4153: Psyr_4153 - conserved hypothetical protein, at 4,938,877 to 4,939,338
_4153
Psyr_4154: Psyr_4154 - microcin-processing peptidase 1, Unknown type peptidase, MEROPS family U62, at 4,940,327 to 4,941,673
_4154
Psyr_4155: Psyr_4155 - Protein of unknown function DUF615, at 4,941,781 to 4,942,302
_4155
Psyr_4156: Psyr_4156 - microcin-processing peptidase 2, Unknown type peptidase, MEROPS family U62, at 4,942,351 to 4,943,790
_4156
Position (kb)
4940
4941
4942 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4939.344 kb on - strand at 4939.344 kb on - strand at 4939.344 kb on - strand at 4939.425 kb on + strand at 4939.425 kb on + strand at 4939.426 kb on - strand at 4939.426 kb on - strand at 4939.426 kb on - strand at 4939.426 kb on - strand at 4939.503 kb on - strand at 4939.863 kb on - strand at 4939.977 kb on - strand at 4939.977 kb on - strand at 4939.977 kb on - strand at 4939.977 kb on - strand at 4940.090 kb on - strand at 4940.121 kb on + strand at 4940.517 kb on + strand, within Psyr_4154 at 4940.518 kb on - strand, within Psyr_4154 at 4940.685 kb on + strand, within Psyr_4154 at 4940.685 kb on + strand, within Psyr_4154 at 4940.686 kb on - strand, within Psyr_4154 at 4940.686 kb on - strand, within Psyr_4154 at 4940.686 kb on - strand, within Psyr_4154 at 4940.700 kb on + strand, within Psyr_4154 at 4940.700 kb on + strand, within Psyr_4154 at 4940.700 kb on + strand, within Psyr_4154 at 4940.700 kb on + strand, within Psyr_4154 at 4940.701 kb on - strand, within Psyr_4154 at 4940.701 kb on - strand, within Psyr_4154 at 4940.701 kb on - strand, within Psyr_4154 at 4940.701 kb on - strand, within Psyr_4154 at 4940.701 kb on - strand, within Psyr_4154 at 4940.701 kb on - strand, within Psyr_4154 at 4940.701 kb on - strand, within Psyr_4154 at 4941.061 kb on - strand, within Psyr_4154 at 4941.066 kb on + strand, within Psyr_4154 at 4941.196 kb on + strand, within Psyr_4154 at 4941.468 kb on + strand, within Psyr_4154 at 4941.469 kb on - strand, within Psyr_4154 at 4941.793 kb on + strand at 4941.793 kb on + strand at 4942.072 kb on + strand, within Psyr_4155 at 4942.072 kb on + strand, within Psyr_4155 at 4942.072 kb on + strand, within Psyr_4155 at 4942.072 kb on + strand, within Psyr_4155 at 4942.072 kb on + strand, within Psyr_4155 at 4942.072 kb on + strand, within Psyr_4155 at 4942.073 kb on - strand, within Psyr_4155 at 4942.073 kb on - strand, within Psyr_4155 at 4942.073 kb on - strand, within Psyr_4155 at 4942.073 kb on - strand, within Psyr_4155 at 4942.261 kb on + strand at 4942.261 kb on + strand at 4942.261 kb on + strand at 4942.261 kb on + strand at 4942.261 kb on + strand at 4942.261 kb on + strand at 4942.262 kb on - strand at 4942.262 kb on - strand at 4942.262 kb on - strand at 4942.262 kb on - strand at 4942.262 kb on - strand at 4942.262 kb on - strand at 4942.395 kb on + strand at 4942.640 kb on + strand, within Psyr_4156 at 4942.640 kb on + strand, within Psyr_4156 at 4942.640 kb on + strand, within Psyr_4156 at 4942.640 kb on + strand, within Psyr_4156 at 4942.641 kb on - strand, within Psyr_4156 at 4942.641 kb on - strand, within Psyr_4156 at 4942.641 kb on - strand, within Psyr_4156 at 4942.641 kb on - strand, within Psyr_4156 at 4942.641 kb on - strand, within Psyr_4156 at 4942.641 kb on - strand, within Psyr_4156 at 4942.641 kb on - strand, within Psyr_4156 at 4942.641 kb on - strand, within Psyr_4156 at 4942.641 kb on - strand, within Psyr_4156
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmine 10 mM remove 4,939,344 - +0.3 4,939,344 - +0.1 4,939,344 - +0.2 4,939,425 + -1.3 4,939,425 + +0.2 4,939,426 - +0.4 4,939,426 - -0.3 4,939,426 - -2.7 4,939,426 - -0.8 4,939,503 - +0.8 4,939,863 - -1.2 4,939,977 - +0.1 4,939,977 - -0.6 4,939,977 - -1.0 4,939,977 - -0.5 4,940,090 - +1.4 4,940,121 + +1.7 4,940,517 + Psyr_4154 0.14 +0.7 4,940,518 - Psyr_4154 0.14 +0.0 4,940,685 + Psyr_4154 0.27 +1.7 4,940,685 + Psyr_4154 0.27 +0.6 4,940,686 - Psyr_4154 0.27 -0.0 4,940,686 - Psyr_4154 0.27 -0.4 4,940,686 - Psyr_4154 0.27 +0.9 4,940,700 + Psyr_4154 0.28 +0.3 4,940,700 + Psyr_4154 0.28 -0.5 4,940,700 + Psyr_4154 0.28 -2.0 4,940,700 + Psyr_4154 0.28 +0.7 4,940,701 - Psyr_4154 0.28 -1.4 4,940,701 - Psyr_4154 0.28 +0.9 4,940,701 - Psyr_4154 0.28 +0.0 4,940,701 - Psyr_4154 0.28 -0.2 4,940,701 - Psyr_4154 0.28 +0.7 4,940,701 - Psyr_4154 0.28 -1.7 4,940,701 - Psyr_4154 0.28 +0.0 4,941,061 - Psyr_4154 0.54 +0.6 4,941,066 + Psyr_4154 0.55 +3.2 4,941,196 + Psyr_4154 0.65 -1.7 4,941,468 + Psyr_4154 0.85 -1.3 4,941,469 - Psyr_4154 0.85 +0.9 4,941,793 + +0.7 4,941,793 + +1.3 4,942,072 + Psyr_4155 0.56 -1.1 4,942,072 + Psyr_4155 0.56 +0.5 4,942,072 + Psyr_4155 0.56 +1.0 4,942,072 + Psyr_4155 0.56 +0.8 4,942,072 + Psyr_4155 0.56 +0.9 4,942,072 + Psyr_4155 0.56 -1.0 4,942,073 - Psyr_4155 0.56 -0.3 4,942,073 - Psyr_4155 0.56 +0.3 4,942,073 - Psyr_4155 0.56 -1.0 4,942,073 - Psyr_4155 0.56 -1.8 4,942,261 + +1.1 4,942,261 + +0.9 4,942,261 + +0.7 4,942,261 + -0.2 4,942,261 + +3.0 4,942,261 + -0.4 4,942,262 - -1.2 4,942,262 - +0.2 4,942,262 - +1.1 4,942,262 - +0.3 4,942,262 - -0.3 4,942,262 - -0.4 4,942,395 + -0.1 4,942,640 + Psyr_4156 0.20 +0.7 4,942,640 + Psyr_4156 0.20 +1.4 4,942,640 + Psyr_4156 0.20 +0.5 4,942,640 + Psyr_4156 0.20 +0.4 4,942,641 - Psyr_4156 0.20 -0.6 4,942,641 - Psyr_4156 0.20 +1.6 4,942,641 - Psyr_4156 0.20 +0.1 4,942,641 - Psyr_4156 0.20 -0.4 4,942,641 - Psyr_4156 0.20 +0.5 4,942,641 - Psyr_4156 0.20 -0.6 4,942,641 - Psyr_4156 0.20 +0.5 4,942,641 - Psyr_4156 0.20 +1.0 4,942,641 - Psyr_4156 0.20 +1.1
Or see this region's nucleotide sequence