Experiment: KB with Harmine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3559 and Psyr_3560 are separated by 11 nucleotides Psyr_3560 and Psyr_3561 are separated by 61 nucleotides Psyr_3561 and Psyr_3562 are separated by 48 nucleotides
Psyr_3559: Psyr_3559 - succinylglutamate desuccinylase, at 4,239,998 to 4,241,005
_3559
Psyr_3560: Psyr_3560 - conserved hypothetical protein, at 4,241,017 to 4,241,304
_3560
Psyr_3561: Psyr_3561 - succinylarginine dihydrolase, at 4,241,366 to 4,242,712
_3561
Psyr_3562: Psyr_3562 - succinylglutamic semialdehyde dehydrogenase, at 4,242,761 to 4,244,227
_3562
Position (kb) 4241
4242
4243 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4240.395 kb on + strand, within Psyr_3559 at 4240.396 kb on - strand, within Psyr_3559 at 4240.396 kb on - strand, within Psyr_3559 at 4240.396 kb on - strand, within Psyr_3559 at 4240.396 kb on - strand, within Psyr_3559 at 4240.401 kb on + strand, within Psyr_3559 at 4240.401 kb on + strand, within Psyr_3559 at 4240.563 kb on + strand, within Psyr_3559 at 4240.747 kb on + strand, within Psyr_3559 at 4240.747 kb on + strand, within Psyr_3559 at 4241.304 kb on + strand at 4241.305 kb on - strand at 4241.306 kb on + strand at 4241.306 kb on + strand at 4241.307 kb on - strand at 4241.313 kb on - strand at 4241.330 kb on + strand at 4241.330 kb on + strand at 4241.330 kb on + strand at 4241.330 kb on + strand at 4241.331 kb on - strand at 4241.331 kb on - strand at 4241.331 kb on - strand at 4241.331 kb on - strand at 4241.331 kb on - strand at 4241.524 kb on - strand, within Psyr_3561 at 4241.695 kb on - strand, within Psyr_3561 at 4241.695 kb on - strand, within Psyr_3561 at 4241.788 kb on - strand, within Psyr_3561 at 4241.922 kb on + strand, within Psyr_3561 at 4241.922 kb on + strand, within Psyr_3561 at 4241.922 kb on + strand, within Psyr_3561 at 4241.969 kb on + strand, within Psyr_3561 at 4241.970 kb on - strand, within Psyr_3561 at 4242.004 kb on + strand, within Psyr_3561 at 4242.005 kb on - strand, within Psyr_3561 at 4242.005 kb on - strand, within Psyr_3561 at 4242.006 kb on + strand, within Psyr_3561 at 4242.006 kb on + strand, within Psyr_3561 at 4242.006 kb on + strand, within Psyr_3561 at 4242.006 kb on + strand, within Psyr_3561 at 4242.006 kb on + strand, within Psyr_3561 at 4242.007 kb on - strand, within Psyr_3561 at 4242.007 kb on - strand, within Psyr_3561 at 4242.306 kb on + strand, within Psyr_3561 at 4242.310 kb on + strand, within Psyr_3561 at 4242.310 kb on + strand, within Psyr_3561 at 4242.310 kb on + strand, within Psyr_3561 at 4242.310 kb on + strand, within Psyr_3561 at 4242.310 kb on + strand, within Psyr_3561 at 4242.311 kb on - strand, within Psyr_3561 at 4242.311 kb on - strand, within Psyr_3561 at 4242.311 kb on - strand, within Psyr_3561 at 4242.646 kb on - strand at 4242.646 kb on - strand at 4242.740 kb on + strand at 4242.855 kb on + strand at 4242.856 kb on - strand at 4242.856 kb on - strand at 4242.859 kb on - strand at 4243.313 kb on - strand, within Psyr_3562 at 4243.437 kb on + strand, within Psyr_3562 at 4243.437 kb on + strand, within Psyr_3562 at 4243.437 kb on + strand, within Psyr_3562 at 4243.437 kb on + strand, within Psyr_3562 at 4243.438 kb on - strand, within Psyr_3562 at 4243.438 kb on - strand, within Psyr_3562 at 4243.438 kb on - strand, within Psyr_3562 at 4243.438 kb on - strand, within Psyr_3562 at 4243.438 kb on - strand, within Psyr_3562
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmine 10 mM remove 4,240,395 + Psyr_3559 0.39 -1.3 4,240,396 - Psyr_3559 0.39 -0.2 4,240,396 - Psyr_3559 0.39 -0.5 4,240,396 - Psyr_3559 0.39 +0.1 4,240,396 - Psyr_3559 0.39 -0.9 4,240,401 + Psyr_3559 0.40 -1.6 4,240,401 + Psyr_3559 0.40 +0.2 4,240,563 + Psyr_3559 0.56 +0.5 4,240,747 + Psyr_3559 0.74 -0.3 4,240,747 + Psyr_3559 0.74 +0.7 4,241,304 + -0.5 4,241,305 - +0.0 4,241,306 + -0.3 4,241,306 + -0.0 4,241,307 - +1.1 4,241,313 - -1.9 4,241,330 + -1.4 4,241,330 + -1.6 4,241,330 + +0.2 4,241,330 + -0.9 4,241,331 - -1.2 4,241,331 - -0.4 4,241,331 - +0.8 4,241,331 - -0.7 4,241,331 - +1.5 4,241,524 - Psyr_3561 0.12 -1.8 4,241,695 - Psyr_3561 0.24 +0.4 4,241,695 - Psyr_3561 0.24 +0.7 4,241,788 - Psyr_3561 0.31 -1.9 4,241,922 + Psyr_3561 0.41 +0.4 4,241,922 + Psyr_3561 0.41 +0.5 4,241,922 + Psyr_3561 0.41 +2.1 4,241,969 + Psyr_3561 0.45 -1.2 4,241,970 - Psyr_3561 0.45 -0.2 4,242,004 + Psyr_3561 0.47 +0.9 4,242,005 - Psyr_3561 0.47 -0.0 4,242,005 - Psyr_3561 0.47 +0.8 4,242,006 + Psyr_3561 0.48 +0.7 4,242,006 + Psyr_3561 0.48 -0.3 4,242,006 + Psyr_3561 0.48 -0.6 4,242,006 + Psyr_3561 0.48 -0.1 4,242,006 + Psyr_3561 0.48 +0.1 4,242,007 - Psyr_3561 0.48 -0.2 4,242,007 - Psyr_3561 0.48 +0.5 4,242,306 + Psyr_3561 0.70 -2.4 4,242,310 + Psyr_3561 0.70 -1.0 4,242,310 + Psyr_3561 0.70 -0.3 4,242,310 + Psyr_3561 0.70 +0.1 4,242,310 + Psyr_3561 0.70 +0.9 4,242,310 + Psyr_3561 0.70 +0.1 4,242,311 - Psyr_3561 0.70 +2.1 4,242,311 - Psyr_3561 0.70 -0.6 4,242,311 - Psyr_3561 0.70 -1.5 4,242,646 - +0.4 4,242,646 - -0.4 4,242,740 + -0.6 4,242,855 + -3.4 4,242,856 - -0.4 4,242,856 - +0.2 4,242,859 - +0.8 4,243,313 - Psyr_3562 0.38 +0.5 4,243,437 + Psyr_3562 0.46 +0.6 4,243,437 + Psyr_3562 0.46 -0.6 4,243,437 + Psyr_3562 0.46 -0.2 4,243,437 + Psyr_3562 0.46 -0.2 4,243,438 - Psyr_3562 0.46 +2.1 4,243,438 - Psyr_3562 0.46 +1.4 4,243,438 - Psyr_3562 0.46 +0.8 4,243,438 - Psyr_3562 0.46 +0.6 4,243,438 - Psyr_3562 0.46 +1.2
Or see this region's nucleotide sequence