Experiment: KB with Harmine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1744 and Psyr_1745 are separated by 85 nucleotides Psyr_1745 and Psyr_1746 are separated by 78 nucleotides Psyr_1746 and Psyr_1747 are separated by 91 nucleotides Psyr_1747 and Psyr_1748 are separated by 106 nucleotides
Psyr_1744: Psyr_1744 - lipoprotein, putative, at 1,981,895 to 1,982,386
_1744
Psyr_1745: Psyr_1745 - hypothetical protein, at 1,982,472 to 1,983,302
_1745
Psyr_1746: Psyr_1746 - Trigger factor, at 1,983,381 to 1,984,691
_1746
Psyr_1747: Psyr_1747 - ATP-dependent Clp protease proteolytic subunit ClpP, at 1,984,783 to 1,985,424
_1747
Psyr_1748: Psyr_1748 - ATP-dependent Clp protease ATP-binding subunit ClpX, at 1,985,531 to 1,986,814
_1748
Position (kb)
1983
1984
1985 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1982.382 kb on + strand at 1982.382 kb on + strand at 1982.382 kb on + strand at 1982.383 kb on - strand at 1982.413 kb on + strand at 1982.413 kb on + strand at 1982.414 kb on - strand at 1982.438 kb on - strand at 1982.446 kb on + strand at 1982.529 kb on + strand at 1982.530 kb on - strand at 1982.530 kb on - strand at 1982.543 kb on + strand at 1982.544 kb on - strand at 1982.544 kb on - strand at 1982.551 kb on + strand at 1982.551 kb on + strand at 1982.551 kb on + strand at 1982.552 kb on - strand at 1982.552 kb on - strand at 1982.552 kb on - strand at 1982.552 kb on - strand at 1982.673 kb on - strand, within Psyr_1745 at 1982.673 kb on - strand, within Psyr_1745 at 1982.694 kb on - strand, within Psyr_1745 at 1982.797 kb on - strand, within Psyr_1745 at 1982.797 kb on - strand, within Psyr_1745 at 1982.797 kb on - strand, within Psyr_1745 at 1982.797 kb on - strand, within Psyr_1745 at 1982.919 kb on + strand, within Psyr_1745 at 1982.919 kb on + strand, within Psyr_1745 at 1982.919 kb on + strand, within Psyr_1745 at 1982.919 kb on + strand, within Psyr_1745 at 1982.920 kb on - strand, within Psyr_1745 at 1982.994 kb on + strand, within Psyr_1745 at 1982.994 kb on + strand, within Psyr_1745 at 1983.085 kb on - strand, within Psyr_1745 at 1983.145 kb on + strand, within Psyr_1745 at 1983.145 kb on + strand, within Psyr_1745 at 1983.186 kb on + strand, within Psyr_1745 at 1983.186 kb on + strand, within Psyr_1745 at 1983.187 kb on - strand, within Psyr_1745 at 1983.187 kb on - strand, within Psyr_1745 at 1983.187 kb on - strand, within Psyr_1745 at 1983.187 kb on - strand, within Psyr_1745 at 1983.187 kb on - strand, within Psyr_1745 at 1983.188 kb on + strand, within Psyr_1745 at 1983.190 kb on + strand, within Psyr_1745 at 1983.190 kb on + strand, within Psyr_1745 at 1983.190 kb on + strand, within Psyr_1745 at 1983.190 kb on + strand, within Psyr_1745 at 1983.191 kb on - strand, within Psyr_1745 at 1983.191 kb on - strand, within Psyr_1745 at 1983.191 kb on - strand, within Psyr_1745 at 1983.191 kb on - strand, within Psyr_1745 at 1983.191 kb on - strand, within Psyr_1745 at 1983.266 kb on + strand at 1983.268 kb on + strand at 1983.269 kb on - strand at 1983.305 kb on - strand at 1983.436 kb on + strand at 1983.437 kb on - strand at 1983.606 kb on + strand, within Psyr_1746 at 1983.606 kb on + strand, within Psyr_1746 at 1983.607 kb on - strand, within Psyr_1746 at 1983.655 kb on + strand, within Psyr_1746 at 1983.713 kb on - strand, within Psyr_1746 at 1983.713 kb on - strand, within Psyr_1746 at 1984.531 kb on + strand, within Psyr_1746 at 1984.542 kb on + strand, within Psyr_1746 at 1984.542 kb on + strand, within Psyr_1746 at 1984.542 kb on + strand, within Psyr_1746 at 1984.570 kb on + strand at 1984.570 kb on + strand at 1984.570 kb on + strand at 1984.570 kb on + strand at 1984.570 kb on + strand at 1984.570 kb on + strand at 1984.600 kb on + strand at 1984.600 kb on + strand at 1984.600 kb on + strand at 1984.600 kb on + strand at 1984.604 kb on + strand at 1984.604 kb on + strand at 1984.798 kb on + strand at 1984.882 kb on + strand, within Psyr_1747 at 1984.891 kb on + strand, within Psyr_1747 at 1984.891 kb on + strand, within Psyr_1747 at 1984.891 kb on + strand, within Psyr_1747 at 1984.892 kb on - strand, within Psyr_1747 at 1984.892 kb on - strand, within Psyr_1747 at 1984.945 kb on + strand, within Psyr_1747 at 1984.945 kb on + strand, within Psyr_1747 at 1984.945 kb on + strand, within Psyr_1747 at 1984.945 kb on + strand, within Psyr_1747 at 1984.945 kb on + strand, within Psyr_1747 at 1984.946 kb on - strand, within Psyr_1747 at 1984.946 kb on - strand, within Psyr_1747 at 1984.946 kb on - strand, within Psyr_1747 at 1985.017 kb on + strand, within Psyr_1747 at 1985.017 kb on + strand, within Psyr_1747 at 1985.018 kb on - strand, within Psyr_1747 at 1985.062 kb on + strand, within Psyr_1747 at 1985.639 kb on - strand at 1985.639 kb on - strand at 1985.639 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmine 10 mM remove 1,982,382 + -0.4 1,982,382 + +1.7 1,982,382 + -0.3 1,982,383 - -0.6 1,982,413 + +0.5 1,982,413 + +1.0 1,982,414 - +0.2 1,982,438 - -1.6 1,982,446 + -0.9 1,982,529 + -0.8 1,982,530 - -0.0 1,982,530 - +2.0 1,982,543 + -0.5 1,982,544 - -0.5 1,982,544 - -1.5 1,982,551 + -0.1 1,982,551 + -0.0 1,982,551 + +0.2 1,982,552 - -0.7 1,982,552 - +0.5 1,982,552 - +1.5 1,982,552 - -0.1 1,982,673 - Psyr_1745 0.24 +0.1 1,982,673 - Psyr_1745 0.24 -0.4 1,982,694 - Psyr_1745 0.27 +1.1 1,982,797 - Psyr_1745 0.39 +0.6 1,982,797 - Psyr_1745 0.39 -0.6 1,982,797 - Psyr_1745 0.39 +0.2 1,982,797 - Psyr_1745 0.39 -0.1 1,982,919 + Psyr_1745 0.54 +2.6 1,982,919 + Psyr_1745 0.54 -0.5 1,982,919 + Psyr_1745 0.54 -0.6 1,982,919 + Psyr_1745 0.54 +0.9 1,982,920 - Psyr_1745 0.54 +0.7 1,982,994 + Psyr_1745 0.63 -0.1 1,982,994 + Psyr_1745 0.63 -1.3 1,983,085 - Psyr_1745 0.74 -1.2 1,983,145 + Psyr_1745 0.81 +0.8 1,983,145 + Psyr_1745 0.81 -1.9 1,983,186 + Psyr_1745 0.86 -0.0 1,983,186 + Psyr_1745 0.86 -0.9 1,983,187 - Psyr_1745 0.86 +0.1 1,983,187 - Psyr_1745 0.86 +1.8 1,983,187 - Psyr_1745 0.86 +0.0 1,983,187 - Psyr_1745 0.86 +1.9 1,983,187 - Psyr_1745 0.86 -0.1 1,983,188 + Psyr_1745 0.86 +0.8 1,983,190 + Psyr_1745 0.86 +2.3 1,983,190 + Psyr_1745 0.86 +1.5 1,983,190 + Psyr_1745 0.86 +0.1 1,983,190 + Psyr_1745 0.86 +0.2 1,983,191 - Psyr_1745 0.87 -0.5 1,983,191 - Psyr_1745 0.87 +0.4 1,983,191 - Psyr_1745 0.87 -2.7 1,983,191 - Psyr_1745 0.87 -0.4 1,983,191 - Psyr_1745 0.87 -1.9 1,983,266 + -0.6 1,983,268 + +0.4 1,983,269 - +0.5 1,983,305 - +2.0 1,983,436 + -0.4 1,983,437 - -0.1 1,983,606 + Psyr_1746 0.17 -1.3 1,983,606 + Psyr_1746 0.17 +0.7 1,983,607 - Psyr_1746 0.17 +0.0 1,983,655 + Psyr_1746 0.21 -0.3 1,983,713 - Psyr_1746 0.25 -0.1 1,983,713 - Psyr_1746 0.25 -1.3 1,984,531 + Psyr_1746 0.88 +0.7 1,984,542 + Psyr_1746 0.89 +0.1 1,984,542 + Psyr_1746 0.89 +1.2 1,984,542 + Psyr_1746 0.89 +0.7 1,984,570 + -0.8 1,984,570 + +1.1 1,984,570 + +0.7 1,984,570 + +0.9 1,984,570 + -0.9 1,984,570 + +0.1 1,984,600 + +0.9 1,984,600 + -0.7 1,984,600 + -0.2 1,984,600 + -0.7 1,984,604 + -0.2 1,984,604 + -1.8 1,984,798 + +1.9 1,984,882 + Psyr_1747 0.15 +0.1 1,984,891 + Psyr_1747 0.17 -0.9 1,984,891 + Psyr_1747 0.17 -1.5 1,984,891 + Psyr_1747 0.17 -0.5 1,984,892 - Psyr_1747 0.17 -1.1 1,984,892 - Psyr_1747 0.17 +0.5 1,984,945 + Psyr_1747 0.25 -1.6 1,984,945 + Psyr_1747 0.25 +0.5 1,984,945 + Psyr_1747 0.25 -0.9 1,984,945 + Psyr_1747 0.25 +1.2 1,984,945 + Psyr_1747 0.25 -2.3 1,984,946 - Psyr_1747 0.25 -0.8 1,984,946 - Psyr_1747 0.25 +1.5 1,984,946 - Psyr_1747 0.25 -1.9 1,985,017 + Psyr_1747 0.36 -2.1 1,985,017 + Psyr_1747 0.36 +1.5 1,985,018 - Psyr_1747 0.37 -1.3 1,985,062 + Psyr_1747 0.43 +0.0 1,985,639 - +1.5 1,985,639 - +1.5 1,985,639 - +1.0
Or see this region's nucleotide sequence