Strain Fitness in Pseudomonas syringae pv. syringae B728a ΔmexB around Psyr_1734

Experiment: KB with Harmine 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_1732 and Psyr_1733 overlap by 4 nucleotidesPsyr_1733 and Psyr_1734 are separated by 207 nucleotidesPsyr_1734 and Psyr_1735 are separated by 24 nucleotides Psyr_1732: Psyr_1732 - UDP-2,3-diacylglucosamine hydrolase, at 1,967,309 to 1,968,055 _1732 Psyr_1733: Psyr_1733 - Peptidyl-prolyl cis-trans isomerase, cyclophilin type, at 1,968,052 to 1,968,555 _1733 Psyr_1734: Psyr_1734 - glutaminyl-tRNA synthetase, at 1,968,763 to 1,970,457 _1734 Psyr_1735: Psyr_1735 - cysteinyl-tRNA synthetase, at 1,970,482 to 1,971,864 _1735 Position (kb) 1968 1969 1970 1971Strain fitness (log2 ratio) -1 0 1at 1968.092 kb on - strandat 1968.306 kb on - strand, within Psyr_1733at 1968.457 kb on - strand, within Psyr_1733at 1968.462 kb on - strand, within Psyr_1733at 1968.462 kb on - strand, within Psyr_1733at 1968.615 kb on + strandat 1968.654 kb on + strandat 1970.463 kb on + strandat 1970.463 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Harmine 10 mM
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1,968,092 - -0.3
1,968,306 - Psyr_1733 0.50 -0.1
1,968,457 - Psyr_1733 0.80 +0.9
1,968,462 - Psyr_1733 0.81 +0.0
1,968,462 - Psyr_1733 0.81 +0.8
1,968,615 + -0.1
1,968,654 + -0.5
1,970,463 + +0.8
1,970,463 + +0.9

Or see this region's nucleotide sequence