Experiment: KB with Harmine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1248 and Psyr_1249 are separated by 22 nucleotides Psyr_1249 and Psyr_1250 are separated by 39 nucleotides Psyr_1250 and Psyr_1251 are separated by 1 nucleotides
Psyr_1248: Psyr_1248 - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase, at 1,403,345 to 1,404,454
_1248
Psyr_1249: Psyr_1249 - histidyl-tRNA synthetase, at 1,404,477 to 1,405,766
_1249
Psyr_1250: Psyr_1250 - conserved hypothetical protein, at 1,405,806 to 1,406,447
_1250
Psyr_1251: Psyr_1251 - quinoprotein, at 1,406,449 to 1,407,594
_1251
Position (kb) 1404
1405
1406 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1404.482 kb on + strand at 1404.482 kb on + strand at 1404.582 kb on + strand at 1404.582 kb on + strand at 1404.678 kb on + strand, within Psyr_1249 at 1404.678 kb on + strand, within Psyr_1249 at 1404.678 kb on + strand, within Psyr_1249 at 1404.798 kb on + strand, within Psyr_1249 at 1405.029 kb on + strand, within Psyr_1249 at 1405.029 kb on + strand, within Psyr_1249 at 1405.230 kb on + strand, within Psyr_1249 at 1405.230 kb on + strand, within Psyr_1249 at 1405.476 kb on + strand, within Psyr_1249 at 1405.775 kb on + strand at 1406.073 kb on + strand, within Psyr_1250 at 1406.073 kb on + strand, within Psyr_1250 at 1406.073 kb on + strand, within Psyr_1250 at 1406.073 kb on + strand, within Psyr_1250 at 1406.349 kb on + strand, within Psyr_1250 at 1406.617 kb on + strand, within Psyr_1251 at 1406.656 kb on + strand, within Psyr_1251
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmine 10 mM remove 1,404,482 + -0.2 1,404,482 + -0.1 1,404,582 + +2.2 1,404,582 + +2.2 1,404,678 + Psyr_1249 0.16 +1.8 1,404,678 + Psyr_1249 0.16 +1.6 1,404,678 + Psyr_1249 0.16 +2.0 1,404,798 + Psyr_1249 0.25 +0.2 1,405,029 + Psyr_1249 0.43 +0.9 1,405,029 + Psyr_1249 0.43 -0.9 1,405,230 + Psyr_1249 0.58 +0.4 1,405,230 + Psyr_1249 0.58 +1.1 1,405,476 + Psyr_1249 0.77 -0.9 1,405,775 + -0.6 1,406,073 + Psyr_1250 0.42 +0.2 1,406,073 + Psyr_1250 0.42 -2.1 1,406,073 + Psyr_1250 0.42 +1.6 1,406,073 + Psyr_1250 0.42 +1.0 1,406,349 + Psyr_1250 0.85 -0.5 1,406,617 + Psyr_1251 0.15 +0.4 1,406,656 + Psyr_1251 0.18 +1.2
Or see this region's nucleotide sequence