Experiment: KB with Harmine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1106 and Psyr_1107 are separated by 59 nucleotides Psyr_1107 and Psyr_1108 are separated by 137 nucleotides Psyr_1108 and Psyr_1109 are separated by 219 nucleotides
Psyr_1106: Psyr_1106 - FecR protein, at 1,253,132 to 1,254,097
_1106
Psyr_1107: Psyr_1107 - Sigma-70 region 2, at 1,254,157 to 1,254,720
_1107
Psyr_1108: Psyr_1108 - glyceraldehyde-3-phosphate dehydrogenase, at 1,254,858 to 1,255,859
_1108
Psyr_1109: Psyr_1109 - 6-phosphogluconate dehydratase, at 1,256,079 to 1,257,905
_1109
Position (kb)
1254
1255
1256 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1253.889 kb on + strand, within Psyr_1106 at 1254.187 kb on + strand at 1254.188 kb on - strand at 1254.223 kb on + strand, within Psyr_1107 at 1254.223 kb on + strand, within Psyr_1107 at 1254.224 kb on - strand, within Psyr_1107 at 1254.266 kb on + strand, within Psyr_1107 at 1254.266 kb on + strand, within Psyr_1107 at 1254.266 kb on + strand, within Psyr_1107 at 1254.267 kb on - strand, within Psyr_1107 at 1254.618 kb on + strand, within Psyr_1107 at 1254.622 kb on + strand, within Psyr_1107 at 1254.622 kb on + strand, within Psyr_1107 at 1254.622 kb on + strand, within Psyr_1107 at 1254.622 kb on + strand, within Psyr_1107 at 1254.622 kb on + strand, within Psyr_1107 at 1254.623 kb on - strand, within Psyr_1107 at 1254.749 kb on - strand at 1254.752 kb on + strand at 1254.752 kb on + strand at 1254.753 kb on - strand at 1254.753 kb on - strand at 1254.753 kb on - strand at 1254.753 kb on - strand at 1254.859 kb on + strand at 1254.925 kb on + strand at 1254.937 kb on - strand at 1255.036 kb on + strand, within Psyr_1108 at 1255.037 kb on - strand, within Psyr_1108 at 1255.283 kb on - strand, within Psyr_1108 at 1255.313 kb on - strand, within Psyr_1108 at 1255.313 kb on - strand, within Psyr_1108 at 1255.365 kb on + strand, within Psyr_1108 at 1255.365 kb on + strand, within Psyr_1108 at 1255.366 kb on - strand, within Psyr_1108 at 1255.366 kb on - strand, within Psyr_1108 at 1255.456 kb on + strand, within Psyr_1108 at 1255.496 kb on + strand, within Psyr_1108 at 1255.496 kb on + strand, within Psyr_1108 at 1255.581 kb on + strand, within Psyr_1108 at 1255.581 kb on + strand, within Psyr_1108 at 1255.581 kb on + strand, within Psyr_1108 at 1255.581 kb on + strand, within Psyr_1108 at 1255.581 kb on + strand, within Psyr_1108 at 1255.581 kb on + strand, within Psyr_1108 at 1255.581 kb on + strand, within Psyr_1108 at 1255.581 kb on + strand, within Psyr_1108 at 1255.582 kb on - strand, within Psyr_1108 at 1255.582 kb on - strand, within Psyr_1108 at 1255.582 kb on - strand, within Psyr_1108 at 1255.582 kb on - strand, within Psyr_1108 at 1255.582 kb on - strand, within Psyr_1108 at 1255.582 kb on - strand, within Psyr_1108 at 1255.582 kb on - strand, within Psyr_1108 at 1255.637 kb on + strand, within Psyr_1108 at 1255.637 kb on + strand, within Psyr_1108 at 1255.637 kb on + strand, within Psyr_1108 at 1255.638 kb on - strand, within Psyr_1108 at 1255.701 kb on + strand, within Psyr_1108 at 1255.701 kb on + strand, within Psyr_1108 at 1255.702 kb on - strand, within Psyr_1108 at 1255.702 kb on - strand, within Psyr_1108 at 1255.795 kb on + strand at 1255.795 kb on + strand at 1255.795 kb on + strand at 1255.795 kb on + strand at 1255.795 kb on + strand at 1255.795 kb on + strand at 1255.795 kb on + strand at 1255.795 kb on + strand at 1255.795 kb on + strand at 1255.796 kb on - strand at 1255.806 kb on + strand at 1255.891 kb on + strand at 1255.960 kb on - strand at 1256.101 kb on + strand at 1256.145 kb on + strand at 1256.299 kb on - strand, within Psyr_1109 at 1256.362 kb on + strand, within Psyr_1109 at 1256.362 kb on + strand, within Psyr_1109 at 1256.363 kb on - strand, within Psyr_1109 at 1256.507 kb on + strand, within Psyr_1109 at 1256.507 kb on + strand, within Psyr_1109 at 1256.507 kb on + strand, within Psyr_1109 at 1256.507 kb on + strand, within Psyr_1109 at 1256.507 kb on + strand, within Psyr_1109 at 1256.507 kb on + strand, within Psyr_1109 at 1256.507 kb on + strand, within Psyr_1109 at 1256.507 kb on + strand, within Psyr_1109 at 1256.508 kb on - strand, within Psyr_1109 at 1256.508 kb on - strand, within Psyr_1109 at 1256.508 kb on - strand, within Psyr_1109 at 1256.508 kb on - strand, within Psyr_1109 at 1256.508 kb on - strand, within Psyr_1109 at 1256.508 kb on - strand, within Psyr_1109 at 1256.508 kb on - strand, within Psyr_1109 at 1256.508 kb on - strand, within Psyr_1109 at 1256.508 kb on - strand, within Psyr_1109 at 1256.657 kb on - strand, within Psyr_1109 at 1256.657 kb on - strand, within Psyr_1109 at 1256.667 kb on + strand, within Psyr_1109 at 1256.828 kb on + strand, within Psyr_1109 at 1256.828 kb on + strand, within Psyr_1109 at 1256.828 kb on + strand, within Psyr_1109 at 1256.828 kb on + strand, within Psyr_1109 at 1256.828 kb on + strand, within Psyr_1109 at 1256.828 kb on + strand, within Psyr_1109 at 1256.829 kb on - strand, within Psyr_1109 at 1256.829 kb on - strand, within Psyr_1109
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmine 10 mM remove 1,253,889 + Psyr_1106 0.78 -0.8 1,254,187 + +0.1 1,254,188 - -1.1 1,254,223 + Psyr_1107 0.12 +0.2 1,254,223 + Psyr_1107 0.12 -0.6 1,254,224 - Psyr_1107 0.12 +1.2 1,254,266 + Psyr_1107 0.19 -0.3 1,254,266 + Psyr_1107 0.19 +0.4 1,254,266 + Psyr_1107 0.19 -0.9 1,254,267 - Psyr_1107 0.20 +0.5 1,254,618 + Psyr_1107 0.82 +0.0 1,254,622 + Psyr_1107 0.82 -0.7 1,254,622 + Psyr_1107 0.82 -0.3 1,254,622 + Psyr_1107 0.82 +0.3 1,254,622 + Psyr_1107 0.82 -0.2 1,254,622 + Psyr_1107 0.82 -0.1 1,254,623 - Psyr_1107 0.83 -1.6 1,254,749 - -0.4 1,254,752 + +0.1 1,254,752 + +0.6 1,254,753 - +0.2 1,254,753 - -1.2 1,254,753 - -0.6 1,254,753 - -1.3 1,254,859 + +0.1 1,254,925 + -0.6 1,254,937 - -1.1 1,255,036 + Psyr_1108 0.18 -0.2 1,255,037 - Psyr_1108 0.18 +0.9 1,255,283 - Psyr_1108 0.42 -1.3 1,255,313 - Psyr_1108 0.45 +0.5 1,255,313 - Psyr_1108 0.45 -0.5 1,255,365 + Psyr_1108 0.51 +0.1 1,255,365 + Psyr_1108 0.51 +0.2 1,255,366 - Psyr_1108 0.51 +0.3 1,255,366 - Psyr_1108 0.51 -0.2 1,255,456 + Psyr_1108 0.60 -0.2 1,255,496 + Psyr_1108 0.64 -0.1 1,255,496 + Psyr_1108 0.64 -1.5 1,255,581 + Psyr_1108 0.72 +0.7 1,255,581 + Psyr_1108 0.72 -1.9 1,255,581 + Psyr_1108 0.72 -1.1 1,255,581 + Psyr_1108 0.72 +0.3 1,255,581 + Psyr_1108 0.72 +0.3 1,255,581 + Psyr_1108 0.72 -0.3 1,255,581 + Psyr_1108 0.72 +0.3 1,255,581 + Psyr_1108 0.72 +0.1 1,255,582 - Psyr_1108 0.72 -2.0 1,255,582 - Psyr_1108 0.72 -0.1 1,255,582 - Psyr_1108 0.72 -0.9 1,255,582 - Psyr_1108 0.72 -0.3 1,255,582 - Psyr_1108 0.72 -1.0 1,255,582 - Psyr_1108 0.72 -0.8 1,255,582 - Psyr_1108 0.72 -1.9 1,255,637 + Psyr_1108 0.78 -1.8 1,255,637 + Psyr_1108 0.78 -0.1 1,255,637 + Psyr_1108 0.78 +1.3 1,255,638 - Psyr_1108 0.78 +1.5 1,255,701 + Psyr_1108 0.84 -0.4 1,255,701 + Psyr_1108 0.84 -0.4 1,255,702 - Psyr_1108 0.84 -2.2 1,255,702 - Psyr_1108 0.84 -0.5 1,255,795 + -0.8 1,255,795 + -1.8 1,255,795 + -0.1 1,255,795 + +0.2 1,255,795 + +0.3 1,255,795 + +0.5 1,255,795 + -1.1 1,255,795 + +0.6 1,255,795 + -0.0 1,255,796 - +0.1 1,255,806 + -2.1 1,255,891 + -0.1 1,255,960 - -0.3 1,256,101 + -2.2 1,256,145 + -1.6 1,256,299 - Psyr_1109 0.12 -0.1 1,256,362 + Psyr_1109 0.15 -0.4 1,256,362 + Psyr_1109 0.15 +0.8 1,256,363 - Psyr_1109 0.16 -1.4 1,256,507 + Psyr_1109 0.23 -1.0 1,256,507 + Psyr_1109 0.23 -0.3 1,256,507 + Psyr_1109 0.23 +1.5 1,256,507 + Psyr_1109 0.23 +1.0 1,256,507 + Psyr_1109 0.23 -1.0 1,256,507 + Psyr_1109 0.23 -1.5 1,256,507 + Psyr_1109 0.23 -1.9 1,256,507 + Psyr_1109 0.23 +0.2 1,256,508 - Psyr_1109 0.23 +1.0 1,256,508 - Psyr_1109 0.23 -1.2 1,256,508 - Psyr_1109 0.23 +3.1 1,256,508 - Psyr_1109 0.23 -1.6 1,256,508 - Psyr_1109 0.23 -1.6 1,256,508 - Psyr_1109 0.23 +0.5 1,256,508 - Psyr_1109 0.23 -1.7 1,256,508 - Psyr_1109 0.23 +0.5 1,256,508 - Psyr_1109 0.23 -0.0 1,256,657 - Psyr_1109 0.32 -1.0 1,256,657 - Psyr_1109 0.32 -0.3 1,256,667 + Psyr_1109 0.32 -0.7 1,256,828 + Psyr_1109 0.41 -0.2 1,256,828 + Psyr_1109 0.41 +0.4 1,256,828 + Psyr_1109 0.41 -1.5 1,256,828 + Psyr_1109 0.41 -2.0 1,256,828 + Psyr_1109 0.41 -1.0 1,256,828 + Psyr_1109 0.41 +0.8 1,256,829 - Psyr_1109 0.41 -0.5 1,256,829 - Psyr_1109 0.41 -1.4
Or see this region's nucleotide sequence