Experiment: KB with Harmine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1085 and Psyr_1086 are separated by 156 nucleotides Psyr_1086 and Psyr_1087 are separated by 123 nucleotides Psyr_1087 and Psyr_1088 overlap by 4 nucleotides
Psyr_1085: Psyr_1085 - 23S rRNA m(6)A-1618 methyltransferase, at 1,226,571 to 1,227,557
_1085
Psyr_1086: Psyr_1086 - valyl-tRNA synthetase, at 1,227,714 to 1,230,560
_1086
Psyr_1087: Psyr_1087 - CDS, at 1,230,684 to 1,230,956
_1087
Psyr_1088: Psyr_1088 - CDS, at 1,230,953 to 1,231,684
_1088
Position (kb) 1227
1228
1229
1230
1231 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1227.115 kb on + strand, within Psyr_1085 at 1227.115 kb on + strand, within Psyr_1085 at 1227.116 kb on - strand, within Psyr_1085 at 1227.116 kb on - strand, within Psyr_1085 at 1227.116 kb on - strand, within Psyr_1085 at 1227.139 kb on + strand, within Psyr_1085 at 1227.139 kb on + strand, within Psyr_1085 at 1227.244 kb on + strand, within Psyr_1085 at 1227.310 kb on + strand, within Psyr_1085 at 1227.595 kb on + strand at 1227.648 kb on + strand at 1227.649 kb on - strand at 1227.649 kb on - strand at 1227.657 kb on - strand at 1227.715 kb on + strand at 1230.603 kb on - strand at 1230.605 kb on - strand at 1230.605 kb on - strand at 1230.664 kb on + strand at 1230.664 kb on + strand at 1230.665 kb on - strand at 1230.719 kb on + strand, within Psyr_1087 at 1230.719 kb on + strand, within Psyr_1087 at 1230.720 kb on - strand, within Psyr_1087 at 1230.720 kb on - strand, within Psyr_1087 at 1230.786 kb on + strand, within Psyr_1087 at 1230.877 kb on + strand, within Psyr_1087 at 1230.878 kb on - strand, within Psyr_1087 at 1231.064 kb on + strand, within Psyr_1088 at 1231.065 kb on - strand, within Psyr_1088 at 1231.070 kb on + strand, within Psyr_1088 at 1231.070 kb on + strand, within Psyr_1088 at 1231.070 kb on + strand, within Psyr_1088 at 1231.071 kb on - strand, within Psyr_1088 at 1231.128 kb on + strand, within Psyr_1088 at 1231.128 kb on + strand, within Psyr_1088 at 1231.128 kb on + strand, within Psyr_1088 at 1231.128 kb on + strand, within Psyr_1088 at 1231.129 kb on - strand, within Psyr_1088 at 1231.129 kb on - strand, within Psyr_1088 at 1231.129 kb on - strand, within Psyr_1088 at 1231.129 kb on - strand, within Psyr_1088 at 1231.170 kb on + strand, within Psyr_1088 at 1231.171 kb on - strand, within Psyr_1088 at 1231.171 kb on - strand, within Psyr_1088 at 1231.219 kb on + strand, within Psyr_1088 at 1231.219 kb on + strand, within Psyr_1088 at 1231.219 kb on + strand, within Psyr_1088 at 1231.222 kb on + strand, within Psyr_1088 at 1231.222 kb on + strand, within Psyr_1088 at 1231.222 kb on + strand, within Psyr_1088 at 1231.222 kb on + strand, within Psyr_1088 at 1231.222 kb on + strand, within Psyr_1088 at 1231.222 kb on + strand, within Psyr_1088 at 1231.222 kb on + strand, within Psyr_1088 at 1231.222 kb on + strand, within Psyr_1088 at 1231.223 kb on - strand, within Psyr_1088 at 1231.223 kb on - strand, within Psyr_1088 at 1231.223 kb on - strand, within Psyr_1088 at 1231.223 kb on - strand, within Psyr_1088 at 1231.263 kb on + strand, within Psyr_1088 at 1231.264 kb on - strand, within Psyr_1088 at 1231.267 kb on + strand, within Psyr_1088 at 1231.267 kb on + strand, within Psyr_1088 at 1231.278 kb on + strand, within Psyr_1088 at 1231.299 kb on + strand, within Psyr_1088 at 1231.300 kb on - strand, within Psyr_1088 at 1231.300 kb on - strand, within Psyr_1088 at 1231.321 kb on + strand, within Psyr_1088 at 1231.322 kb on - strand, within Psyr_1088 at 1231.322 kb on - strand, within Psyr_1088 at 1231.324 kb on - strand, within Psyr_1088 at 1231.353 kb on + strand, within Psyr_1088 at 1231.353 kb on + strand, within Psyr_1088 at 1231.353 kb on + strand, within Psyr_1088 at 1231.354 kb on - strand, within Psyr_1088 at 1231.354 kb on - strand, within Psyr_1088 at 1231.354 kb on - strand, within Psyr_1088 at 1231.354 kb on - strand, within Psyr_1088 at 1231.354 kb on - strand, within Psyr_1088 at 1231.354 kb on - strand, within Psyr_1088 at 1231.486 kb on + strand, within Psyr_1088 at 1231.522 kb on + strand, within Psyr_1088 at 1231.522 kb on + strand, within Psyr_1088 at 1231.523 kb on - strand, within Psyr_1088 at 1231.560 kb on + strand, within Psyr_1088
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmine 10 mM remove 1,227,115 + Psyr_1085 0.55 -0.6 1,227,115 + Psyr_1085 0.55 +0.9 1,227,116 - Psyr_1085 0.55 -0.3 1,227,116 - Psyr_1085 0.55 -0.6 1,227,116 - Psyr_1085 0.55 +0.3 1,227,139 + Psyr_1085 0.58 +0.4 1,227,139 + Psyr_1085 0.58 +0.9 1,227,244 + Psyr_1085 0.68 -0.0 1,227,310 + Psyr_1085 0.75 +1.3 1,227,595 + +0.5 1,227,648 + +1.1 1,227,649 - +1.1 1,227,649 - +0.1 1,227,657 - -0.2 1,227,715 + -2.3 1,230,603 - +0.4 1,230,605 - +0.1 1,230,605 - -1.0 1,230,664 + -1.6 1,230,664 + +1.0 1,230,665 - -2.0 1,230,719 + Psyr_1087 0.13 +2.2 1,230,719 + Psyr_1087 0.13 -2.7 1,230,720 - Psyr_1087 0.13 +0.6 1,230,720 - Psyr_1087 0.13 +0.7 1,230,786 + Psyr_1087 0.37 +0.6 1,230,877 + Psyr_1087 0.71 +0.6 1,230,878 - Psyr_1087 0.71 -1.1 1,231,064 + Psyr_1088 0.15 -1.3 1,231,065 - Psyr_1088 0.15 +0.5 1,231,070 + Psyr_1088 0.16 +3.4 1,231,070 + Psyr_1088 0.16 +1.2 1,231,070 + Psyr_1088 0.16 -0.0 1,231,071 - Psyr_1088 0.16 +0.2 1,231,128 + Psyr_1088 0.24 -0.9 1,231,128 + Psyr_1088 0.24 +0.2 1,231,128 + Psyr_1088 0.24 -2.6 1,231,128 + Psyr_1088 0.24 +0.0 1,231,129 - Psyr_1088 0.24 -1.6 1,231,129 - Psyr_1088 0.24 -1.3 1,231,129 - Psyr_1088 0.24 +0.5 1,231,129 - Psyr_1088 0.24 -0.0 1,231,170 + Psyr_1088 0.30 -0.0 1,231,171 - Psyr_1088 0.30 +0.9 1,231,171 - Psyr_1088 0.30 +0.6 1,231,219 + Psyr_1088 0.36 +0.6 1,231,219 + Psyr_1088 0.36 +1.0 1,231,219 + Psyr_1088 0.36 -0.1 1,231,222 + Psyr_1088 0.37 +2.3 1,231,222 + Psyr_1088 0.37 -0.6 1,231,222 + Psyr_1088 0.37 +0.5 1,231,222 + Psyr_1088 0.37 +1.0 1,231,222 + Psyr_1088 0.37 +0.7 1,231,222 + Psyr_1088 0.37 +0.9 1,231,222 + Psyr_1088 0.37 -1.6 1,231,222 + Psyr_1088 0.37 -0.1 1,231,223 - Psyr_1088 0.37 +0.3 1,231,223 - Psyr_1088 0.37 +1.5 1,231,223 - Psyr_1088 0.37 -0.5 1,231,223 - Psyr_1088 0.37 -0.0 1,231,263 + Psyr_1088 0.42 -1.1 1,231,264 - Psyr_1088 0.42 -0.8 1,231,267 + Psyr_1088 0.43 -2.2 1,231,267 + Psyr_1088 0.43 -0.1 1,231,278 + Psyr_1088 0.44 +0.6 1,231,299 + Psyr_1088 0.47 -1.3 1,231,300 - Psyr_1088 0.47 -1.0 1,231,300 - Psyr_1088 0.47 -0.1 1,231,321 + Psyr_1088 0.50 -0.2 1,231,322 - Psyr_1088 0.50 +2.9 1,231,322 - Psyr_1088 0.50 -0.0 1,231,324 - Psyr_1088 0.51 -2.0 1,231,353 + Psyr_1088 0.55 -0.7 1,231,353 + Psyr_1088 0.55 -0.3 1,231,353 + Psyr_1088 0.55 +0.4 1,231,354 - Psyr_1088 0.55 -0.8 1,231,354 - Psyr_1088 0.55 +0.2 1,231,354 - Psyr_1088 0.55 -0.3 1,231,354 - Psyr_1088 0.55 -0.9 1,231,354 - Psyr_1088 0.55 -1.5 1,231,354 - Psyr_1088 0.55 -0.8 1,231,486 + Psyr_1088 0.73 +0.0 1,231,522 + Psyr_1088 0.78 -2.3 1,231,522 + Psyr_1088 0.78 +0.9 1,231,523 - Psyr_1088 0.78 +0.1 1,231,560 + Psyr_1088 0.83 +0.5
Or see this region's nucleotide sequence