Experiment: KB with Harmine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0578 and Psyr_RNA2 are separated by 106 nucleotides Psyr_RNA2 and Psyr_RNA3 are separated by 160 nucleotides Psyr_RNA3 and Psyr_0579 are separated by 225 nucleotides Psyr_0579 and Psyr_0580 overlap by 4 nucleotides
Psyr_0578: Psyr_0578 - Histidine kinase, HAMP region:Bacterial chemotaxis sensory transducer, at 657,338 to 659,272
_0578
Psyr_RNA2: Psyr_RNA2 - tRNA-Leu, at 659,379 to 659,465
_RNA2
Psyr_RNA3: Psyr_RNA3 - tRNA-Leu, at 659,626 to 659,712
_RNA3
Psyr_0579: Psyr_0579 - RNAse R, at 659,938 to 662,571
_0579
Psyr_0580: Psyr_0580 - 23S rRNA Gm-2251 2'-O-methyltransferase, at 662,568 to 663,323
_0580
Position (kb) 659
660
661
662
663 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 659.019 kb on + strand, within Psyr_0578 at 659.019 kb on + strand, within Psyr_0578 at 659.020 kb on - strand, within Psyr_0578 at 659.502 kb on + strand at 659.502 kb on + strand at 659.503 kb on - strand at 659.505 kb on - strand at 659.505 kb on - strand at 659.518 kb on + strand at 659.519 kb on - strand at 659.525 kb on + strand at 659.546 kb on + strand at 659.610 kb on - strand at 659.610 kb on - strand at 659.610 kb on - strand at 659.610 kb on - strand at 659.610 kb on - strand at 659.610 kb on - strand at 659.610 kb on - strand at 659.614 kb on + strand at 659.615 kb on - strand at 659.615 kb on - strand at 659.749 kb on + strand at 659.895 kb on + strand at 659.905 kb on + strand at 659.912 kb on + strand at 659.912 kb on + strand at 659.912 kb on + strand at 659.914 kb on + strand at 659.914 kb on + strand at 659.914 kb on + strand at 659.914 kb on + strand at 659.914 kb on + strand at 659.914 kb on + strand at 659.914 kb on + strand at 659.914 kb on + strand at 659.914 kb on + strand at 659.914 kb on + strand at 659.915 kb on - strand at 659.915 kb on - strand at 659.915 kb on - strand at 659.915 kb on - strand at 659.915 kb on - strand at 659.915 kb on - strand at 659.915 kb on - strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.916 kb on + strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.917 kb on - strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.918 kb on + strand at 659.919 kb on - strand at 659.919 kb on - strand at 659.919 kb on - strand at 659.919 kb on - strand at 659.936 kb on + strand at 659.936 kb on + strand at 659.937 kb on - strand at 659.937 kb on - strand at 660.001 kb on - strand at 660.157 kb on + strand at 660.157 kb on + strand at 660.175 kb on + strand at 660.175 kb on + strand at 660.176 kb on - strand at 660.415 kb on + strand, within Psyr_0579 at 660.598 kb on + strand, within Psyr_0579 at 660.598 kb on + strand, within Psyr_0579 at 660.598 kb on + strand, within Psyr_0579 at 660.598 kb on + strand, within Psyr_0579 at 660.598 kb on + strand, within Psyr_0579 at 660.598 kb on + strand, within Psyr_0579 at 660.598 kb on + strand, within Psyr_0579 at 660.598 kb on + strand, within Psyr_0579 at 660.599 kb on - strand, within Psyr_0579 at 660.599 kb on - strand, within Psyr_0579 at 660.599 kb on - strand, within Psyr_0579 at 660.599 kb on - strand, within Psyr_0579 at 660.636 kb on + strand, within Psyr_0579 at 660.636 kb on + strand, within Psyr_0579 at 660.636 kb on + strand, within Psyr_0579 at 660.637 kb on - strand, within Psyr_0579 at 660.641 kb on - strand, within Psyr_0579 at 660.799 kb on + strand, within Psyr_0579 at 660.799 kb on + strand, within Psyr_0579 at 660.881 kb on - strand, within Psyr_0579 at 661.069 kb on + strand, within Psyr_0579 at 661.114 kb on + strand, within Psyr_0579 at 661.114 kb on + strand, within Psyr_0579 at 661.115 kb on - strand, within Psyr_0579 at 661.115 kb on - strand, within Psyr_0579 at 661.213 kb on + strand, within Psyr_0579 at 661.213 kb on + strand, within Psyr_0579 at 661.214 kb on - strand, within Psyr_0579 at 661.214 kb on - strand, within Psyr_0579 at 661.214 kb on - strand, within Psyr_0579 at 661.214 kb on - strand, within Psyr_0579 at 661.222 kb on + strand, within Psyr_0579 at 661.222 kb on + strand, within Psyr_0579 at 661.222 kb on + strand, within Psyr_0579 at 661.222 kb on + strand, within Psyr_0579 at 661.223 kb on - strand, within Psyr_0579 at 661.223 kb on - strand, within Psyr_0579 at 661.223 kb on - strand, within Psyr_0579 at 661.223 kb on - strand, within Psyr_0579 at 661.223 kb on - strand, within Psyr_0579 at 661.223 kb on - strand, within Psyr_0579 at 661.223 kb on - strand, within Psyr_0579 at 661.229 kb on + strand, within Psyr_0579 at 661.229 kb on + strand, within Psyr_0579 at 661.230 kb on - strand, within Psyr_0579 at 661.339 kb on - strand, within Psyr_0579 at 661.430 kb on - strand, within Psyr_0579 at 661.672 kb on + strand, within Psyr_0579 at 661.672 kb on + strand, within Psyr_0579 at 661.672 kb on + strand, within Psyr_0579 at 661.672 kb on + strand, within Psyr_0579 at 661.896 kb on - strand, within Psyr_0579 at 661.918 kb on + strand, within Psyr_0579 at 661.918 kb on + strand, within Psyr_0579 at 661.918 kb on + strand, within Psyr_0579 at 661.918 kb on + strand, within Psyr_0579 at 661.918 kb on + strand, within Psyr_0579 at 661.919 kb on - strand, within Psyr_0579 at 661.967 kb on + strand, within Psyr_0579 at 662.001 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.047 kb on + strand, within Psyr_0579 at 662.048 kb on - strand, within Psyr_0579 at 662.048 kb on - strand, within Psyr_0579 at 662.050 kb on + strand, within Psyr_0579 at 662.051 kb on - strand, within Psyr_0579 at 662.227 kb on - strand, within Psyr_0579 at 662.227 kb on - strand, within Psyr_0579 at 662.299 kb on - strand, within Psyr_0579 at 662.373 kb on + strand at 662.421 kb on + strand at 662.550 kb on + strand at 662.550 kb on + strand at 662.589 kb on + strand at 662.590 kb on - strand at 662.605 kb on + strand at 662.606 kb on - strand at 662.606 kb on - strand at 662.606 kb on - strand at 663.152 kb on + strand, within Psyr_0580 at 663.152 kb on + strand, within Psyr_0580 at 663.152 kb on + strand, within Psyr_0580 at 663.207 kb on + strand, within Psyr_0580 at 663.321 kb on + strand at 663.322 kb on - strand at 663.322 kb on - strand at 663.324 kb on + strand at 663.324 kb on + strand at 663.324 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.353 kb on - strand at 663.353 kb on - strand at 663.353 kb on - strand at 663.359 kb on + strand at 663.359 kb on + strand at 663.359 kb on + strand at 663.359 kb on + strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.376 kb on + strand at 663.376 kb on + strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.382 kb on + strand at 663.382 kb on + strand at 663.383 kb on - strand at 663.383 kb on - strand at 663.383 kb on - strand at 663.383 kb on - strand at 663.421 kb on + strand at 663.421 kb on + strand at 663.422 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmine 10 mM remove 659,019 + Psyr_0578 0.87 +0.3 659,019 + Psyr_0578 0.87 +1.5 659,020 - Psyr_0578 0.87 +1.5 659,502 + -1.0 659,502 + +0.5 659,503 - -0.8 659,505 - +0.1 659,505 - +0.0 659,518 + +0.5 659,519 - -1.4 659,525 + -0.9 659,546 + -0.3 659,610 - +0.1 659,610 - +0.5 659,610 - +1.1 659,610 - -0.5 659,610 - +0.4 659,610 - -0.3 659,610 - +0.3 659,614 + -0.1 659,615 - -0.3 659,615 - -1.6 659,749 + +0.9 659,895 + +0.5 659,905 + -1.1 659,912 + +0.3 659,912 + -1.4 659,912 + +0.3 659,914 + +0.6 659,914 + -0.4 659,914 + -2.3 659,914 + -1.3 659,914 + -0.2 659,914 + +0.5 659,914 + -1.4 659,914 + -0.1 659,914 + +0.1 659,914 + -1.8 659,915 - -1.5 659,915 - -0.9 659,915 - -1.1 659,915 - -0.9 659,915 - +0.0 659,915 - +0.5 659,915 - -2.9 659,916 + +0.2 659,916 + -1.8 659,916 + -1.6 659,916 + -1.6 659,916 + -0.7 659,916 + +4.1 659,916 + +0.2 659,916 + -0.6 659,916 + -1.5 659,916 + -0.3 659,916 + +0.4 659,916 + -1.1 659,916 + -1.1 659,916 + +0.1 659,916 + -0.9 659,916 + +0.9 659,916 + +0.5 659,916 + +0.6 659,916 + +0.1 659,916 + +0.2 659,916 + +0.1 659,916 + -1.1 659,916 + -1.9 659,916 + +0.5 659,917 - -2.5 659,917 - -1.1 659,917 - -0.5 659,917 - -0.7 659,917 - +0.1 659,917 - -1.3 659,917 - -1.6 659,918 + +0.3 659,918 + +0.1 659,918 + -1.9 659,918 + +0.4 659,918 + -0.8 659,918 + -0.6 659,918 + +1.3 659,918 + -1.4 659,918 + -0.3 659,918 + +0.5 659,918 + +0.3 659,919 - -2.0 659,919 - -0.7 659,919 - -1.3 659,919 - -1.1 659,936 + +0.3 659,936 + -0.3 659,937 - -0.8 659,937 - -2.2 660,001 - -1.0 660,157 + +0.9 660,157 + -0.6 660,175 + -2.1 660,175 + -0.8 660,176 - -3.8 660,415 + Psyr_0579 0.18 -1.3 660,598 + Psyr_0579 0.25 -0.4 660,598 + Psyr_0579 0.25 -1.4 660,598 + Psyr_0579 0.25 -0.3 660,598 + Psyr_0579 0.25 -1.0 660,598 + Psyr_0579 0.25 +0.5 660,598 + Psyr_0579 0.25 -1.7 660,598 + Psyr_0579 0.25 -1.1 660,598 + Psyr_0579 0.25 -1.7 660,599 - Psyr_0579 0.25 -0.3 660,599 - Psyr_0579 0.25 -1.0 660,599 - Psyr_0579 0.25 +0.1 660,599 - Psyr_0579 0.25 -2.5 660,636 + Psyr_0579 0.26 -1.2 660,636 + Psyr_0579 0.26 +1.5 660,636 + Psyr_0579 0.26 -1.9 660,637 - Psyr_0579 0.27 -1.9 660,641 - Psyr_0579 0.27 -0.5 660,799 + Psyr_0579 0.33 -1.3 660,799 + Psyr_0579 0.33 +0.2 660,881 - Psyr_0579 0.36 -1.7 661,069 + Psyr_0579 0.43 -3.2 661,114 + Psyr_0579 0.45 -2.7 661,114 + Psyr_0579 0.45 -2.1 661,115 - Psyr_0579 0.45 -0.8 661,115 - Psyr_0579 0.45 +0.5 661,213 + Psyr_0579 0.48 -2.5 661,213 + Psyr_0579 0.48 -0.6 661,214 - Psyr_0579 0.48 +0.1 661,214 - Psyr_0579 0.48 -0.5 661,214 - Psyr_0579 0.48 +0.3 661,214 - Psyr_0579 0.48 -1.7 661,222 + Psyr_0579 0.49 -1.3 661,222 + Psyr_0579 0.49 -0.5 661,222 + Psyr_0579 0.49 -1.3 661,222 + Psyr_0579 0.49 -0.9 661,223 - Psyr_0579 0.49 -2.1 661,223 - Psyr_0579 0.49 -0.4 661,223 - Psyr_0579 0.49 -0.1 661,223 - Psyr_0579 0.49 +0.1 661,223 - Psyr_0579 0.49 -0.3 661,223 - Psyr_0579 0.49 -0.6 661,223 - Psyr_0579 0.49 -2.2 661,229 + Psyr_0579 0.49 -0.5 661,229 + Psyr_0579 0.49 -1.3 661,230 - Psyr_0579 0.49 +0.5 661,339 - Psyr_0579 0.53 -0.5 661,430 - Psyr_0579 0.57 -2.1 661,672 + Psyr_0579 0.66 -0.9 661,672 + Psyr_0579 0.66 +0.5 661,672 + Psyr_0579 0.66 +0.7 661,672 + Psyr_0579 0.66 -2.0 661,896 - Psyr_0579 0.74 -0.5 661,918 + Psyr_0579 0.75 -1.9 661,918 + Psyr_0579 0.75 -2.0 661,918 + Psyr_0579 0.75 -0.9 661,918 + Psyr_0579 0.75 -0.5 661,918 + Psyr_0579 0.75 +2.8 661,919 - Psyr_0579 0.75 -1.7 661,967 + Psyr_0579 0.77 -2.3 662,001 + Psyr_0579 0.78 -0.5 662,005 + Psyr_0579 0.78 -1.7 662,005 + Psyr_0579 0.78 -2.4 662,005 + Psyr_0579 0.78 -0.5 662,005 + Psyr_0579 0.78 -0.3 662,005 + Psyr_0579 0.78 -0.3 662,005 + Psyr_0579 0.78 +0.7 662,005 + Psyr_0579 0.78 +1.2 662,005 + Psyr_0579 0.78 -1.0 662,005 + Psyr_0579 0.78 -1.1 662,005 + Psyr_0579 0.78 -0.3 662,005 + Psyr_0579 0.78 -1.1 662,005 + Psyr_0579 0.78 -0.9 662,005 + Psyr_0579 0.78 -1.4 662,005 + Psyr_0579 0.78 -1.0 662,005 + Psyr_0579 0.78 -1.4 662,006 - Psyr_0579 0.79 -0.5 662,006 - Psyr_0579 0.79 +0.1 662,006 - Psyr_0579 0.79 -0.4 662,006 - Psyr_0579 0.79 -0.1 662,006 - Psyr_0579 0.79 +0.3 662,006 - Psyr_0579 0.79 -1.1 662,006 - Psyr_0579 0.79 +0.7 662,006 - Psyr_0579 0.79 +0.5 662,006 - Psyr_0579 0.79 -1.1 662,047 + Psyr_0579 0.80 +0.2 662,048 - Psyr_0579 0.80 -0.1 662,048 - Psyr_0579 0.80 -0.3 662,050 + Psyr_0579 0.80 -1.5 662,051 - Psyr_0579 0.80 +1.1 662,227 - Psyr_0579 0.87 -0.2 662,227 - Psyr_0579 0.87 -0.1 662,299 - Psyr_0579 0.90 -0.2 662,373 + +1.1 662,421 + +2.1 662,550 + -0.1 662,550 + +0.5 662,589 + -1.3 662,590 - -2.1 662,605 + -0.4 662,606 - -0.7 662,606 - +0.3 662,606 - +1.1 663,152 + Psyr_0580 0.77 -1.1 663,152 + Psyr_0580 0.77 +0.6 663,152 + Psyr_0580 0.77 -0.5 663,207 + Psyr_0580 0.85 -1.1 663,321 + +0.4 663,322 - +0.4 663,322 - -0.1 663,324 + +0.4 663,324 + +0.0 663,324 + +0.9 663,352 + +0.0 663,352 + -1.7 663,352 + -0.2 663,353 - +0.4 663,353 - +0.1 663,353 - +0.9 663,359 + +0.4 663,359 + +0.4 663,359 + +0.1 663,359 + -0.3 663,360 - -1.5 663,360 - +0.6 663,360 - -0.5 663,360 - +0.3 663,360 - -1.0 663,360 - +0.6 663,376 + -0.1 663,376 + +0.5 663,377 - -0.2 663,377 - -0.5 663,377 - -1.0 663,377 - -2.0 663,377 - +1.2 663,377 - +0.9 663,377 - -0.8 663,377 - +0.5 663,382 + +0.5 663,382 + -1.0 663,383 - -0.8 663,383 - +0.3 663,383 - +0.8 663,383 - +0.9 663,421 + -0.1 663,421 + -1.1 663,422 - -1.8
Or see this region's nucleotide sequence