Experiment: KB with Harmine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0542 and Psyr_0543 are separated by 226 nucleotides Psyr_0543 and Psyr_0544 are separated by 426 nucleotides
Psyr_0542: Psyr_0542 - Three-deoxy-D-manno-octulosonic-acid transferase, N-terminal, at 611,935 to 613,080
_0542
Psyr_0543: Psyr_0543 - Type I secretion outer membrane protein, TolC, at 613,307 to 614,746
_0543
Psyr_0544: Psyr_0544 - hydroxymethylpyrimidine synthase, at 615,173 to 617,062
_0544
Position (kb) 613
614
615 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6
7
8 at 613.137 kb on - strand at 613.137 kb on - strand at 613.386 kb on + strand at 613.386 kb on + strand at 613.386 kb on + strand at 613.476 kb on + strand, within Psyr_0543 at 613.476 kb on + strand, within Psyr_0543 at 613.476 kb on + strand, within Psyr_0543 at 613.476 kb on + strand, within Psyr_0543 at 613.477 kb on - strand, within Psyr_0543 at 613.477 kb on - strand, within Psyr_0543 at 613.477 kb on - strand, within Psyr_0543 at 613.497 kb on + strand, within Psyr_0543 at 613.497 kb on + strand, within Psyr_0543 at 613.498 kb on - strand, within Psyr_0543 at 613.498 kb on - strand, within Psyr_0543 at 613.515 kb on + strand, within Psyr_0543 at 613.515 kb on + strand, within Psyr_0543 at 613.515 kb on + strand, within Psyr_0543 at 613.515 kb on + strand, within Psyr_0543 at 613.515 kb on + strand, within Psyr_0543 at 613.515 kb on + strand, within Psyr_0543 at 613.515 kb on + strand, within Psyr_0543 at 613.515 kb on + strand, within Psyr_0543 at 613.515 kb on + strand, within Psyr_0543 at 613.516 kb on - strand, within Psyr_0543 at 613.516 kb on - strand, within Psyr_0543 at 613.748 kb on + strand, within Psyr_0543 at 613.776 kb on + strand, within Psyr_0543 at 613.776 kb on + strand, within Psyr_0543 at 613.845 kb on + strand, within Psyr_0543 at 613.845 kb on + strand, within Psyr_0543 at 613.845 kb on + strand, within Psyr_0543 at 613.845 kb on + strand, within Psyr_0543 at 613.845 kb on + strand, within Psyr_0543 at 613.845 kb on + strand, within Psyr_0543 at 613.845 kb on + strand, within Psyr_0543 at 613.845 kb on + strand, within Psyr_0543 at 613.845 kb on + strand, within Psyr_0543 at 613.846 kb on - strand, within Psyr_0543 at 613.846 kb on - strand, within Psyr_0543 at 613.846 kb on - strand, within Psyr_0543 at 613.846 kb on - strand, within Psyr_0543 at 613.890 kb on + strand, within Psyr_0543 at 613.891 kb on - strand, within Psyr_0543 at 613.891 kb on - strand, within Psyr_0543 at 613.891 kb on - strand, within Psyr_0543 at 613.891 kb on - strand, within Psyr_0543 at 613.968 kb on + strand, within Psyr_0543 at 613.969 kb on - strand, within Psyr_0543 at 613.969 kb on - strand, within Psyr_0543 at 614.085 kb on + strand, within Psyr_0543 at 614.173 kb on - strand, within Psyr_0543 at 614.173 kb on - strand, within Psyr_0543 at 614.306 kb on + strand, within Psyr_0543 at 614.306 kb on + strand, within Psyr_0543 at 614.316 kb on + strand, within Psyr_0543 at 614.316 kb on + strand, within Psyr_0543 at 614.316 kb on + strand, within Psyr_0543 at 614.317 kb on - strand, within Psyr_0543 at 614.317 kb on - strand, within Psyr_0543 at 614.640 kb on + strand at 614.774 kb on - strand at 614.777 kb on + strand at 614.777 kb on + strand at 614.778 kb on - strand at 614.814 kb on + strand at 614.815 kb on - strand at 614.815 kb on - strand at 614.815 kb on - strand at 614.857 kb on + strand at 614.876 kb on - strand at 614.904 kb on + strand at 614.904 kb on + strand at 614.905 kb on - strand at 614.937 kb on + strand at 614.937 kb on + strand at 614.937 kb on + strand at 615.062 kb on + strand at 615.142 kb on - strand at 615.142 kb on - strand at 615.142 kb on - strand at 615.142 kb on - strand at 615.142 kb on - strand at 615.142 kb on - strand at 615.178 kb on + strand at 615.178 kb on + strand at 615.179 kb on - strand at 615.179 kb on - strand at 615.180 kb on + strand at 615.180 kb on + strand at 615.180 kb on + strand at 615.180 kb on + strand at 615.180 kb on + strand at 615.180 kb on + strand at 615.180 kb on + strand at 615.180 kb on + strand at 615.181 kb on - strand at 615.181 kb on - strand at 615.181 kb on - strand at 615.267 kb on - strand at 615.371 kb on + strand, within Psyr_0544 at 615.371 kb on + strand, within Psyr_0544 at 615.372 kb on - strand, within Psyr_0544 at 615.372 kb on - strand, within Psyr_0544 at 615.372 kb on - strand, within Psyr_0544 at 615.606 kb on - strand, within Psyr_0544 at 615.641 kb on + strand, within Psyr_0544 at 615.641 kb on + strand, within Psyr_0544 at 615.641 kb on + strand, within Psyr_0544 at 615.642 kb on - strand, within Psyr_0544 at 615.642 kb on - strand, within Psyr_0544 at 615.733 kb on + strand, within Psyr_0544
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmine 10 mM remove 613,137 - -0.7 613,137 - +0.1 613,386 + -2.2 613,386 + -2.4 613,386 + -0.7 613,476 + Psyr_0543 0.12 -1.9 613,476 + Psyr_0543 0.12 -1.4 613,476 + Psyr_0543 0.12 -2.6 613,476 + Psyr_0543 0.12 -2.7 613,477 - Psyr_0543 0.12 -0.2 613,477 - Psyr_0543 0.12 -0.9 613,477 - Psyr_0543 0.12 -3.6 613,497 + Psyr_0543 0.13 +0.4 613,497 + Psyr_0543 0.13 +0.4 613,498 - Psyr_0543 0.13 -0.4 613,498 - Psyr_0543 0.13 -1.7 613,515 + Psyr_0543 0.14 -0.7 613,515 + Psyr_0543 0.14 -4.6 613,515 + Psyr_0543 0.14 -2.4 613,515 + Psyr_0543 0.14 -2.3 613,515 + Psyr_0543 0.14 -2.3 613,515 + Psyr_0543 0.14 +1.4 613,515 + Psyr_0543 0.14 -2.2 613,515 + Psyr_0543 0.14 -1.7 613,515 + Psyr_0543 0.14 -2.2 613,516 - Psyr_0543 0.15 -1.8 613,516 - Psyr_0543 0.15 -2.4 613,748 + Psyr_0543 0.31 -2.3 613,776 + Psyr_0543 0.33 -0.4 613,776 + Psyr_0543 0.33 -1.4 613,845 + Psyr_0543 0.37 -1.7 613,845 + Psyr_0543 0.37 +0.9 613,845 + Psyr_0543 0.37 +0.7 613,845 + Psyr_0543 0.37 -1.2 613,845 + Psyr_0543 0.37 -1.4 613,845 + Psyr_0543 0.37 -1.7 613,845 + Psyr_0543 0.37 -3.2 613,845 + Psyr_0543 0.37 -0.9 613,845 + Psyr_0543 0.37 -1.2 613,846 - Psyr_0543 0.37 -0.9 613,846 - Psyr_0543 0.37 -3.0 613,846 - Psyr_0543 0.37 -1.5 613,846 - Psyr_0543 0.37 +0.7 613,890 + Psyr_0543 0.40 -3.4 613,891 - Psyr_0543 0.41 -0.4 613,891 - Psyr_0543 0.41 -2.7 613,891 - Psyr_0543 0.41 -0.6 613,891 - Psyr_0543 0.41 -2.5 613,968 + Psyr_0543 0.46 -2.7 613,969 - Psyr_0543 0.46 -2.3 613,969 - Psyr_0543 0.46 -3.3 614,085 + Psyr_0543 0.54 +2.0 614,173 - Psyr_0543 0.60 -2.2 614,173 - Psyr_0543 0.60 +0.2 614,306 + Psyr_0543 0.69 -0.3 614,306 + Psyr_0543 0.69 -1.7 614,316 + Psyr_0543 0.70 -1.4 614,316 + Psyr_0543 0.70 -2.4 614,316 + Psyr_0543 0.70 -2.9 614,317 - Psyr_0543 0.70 -1.4 614,317 - Psyr_0543 0.70 +1.2 614,640 + -3.4 614,774 - +5.2 614,777 + -0.3 614,777 + -0.9 614,778 - +3.1 614,814 + -1.3 614,815 - +6.6 614,815 - +6.5 614,815 - +8.1 614,857 + -2.5 614,876 - +6.4 614,904 + -1.3 614,904 + -2.5 614,905 - +5.1 614,937 + -1.6 614,937 + -1.4 614,937 + -0.9 615,062 + -2.3 615,142 - +4.6 615,142 - +5.4 615,142 - +4.8 615,142 - +5.0 615,142 - +4.9 615,142 - +5.1 615,178 + -2.8 615,178 + -1.7 615,179 - +4.2 615,179 - +4.3 615,180 + +0.1 615,180 + -2.9 615,180 + -1.3 615,180 + -0.1 615,180 + -1.2 615,180 + -0.9 615,180 + -1.0 615,180 + +0.1 615,181 - +3.4 615,181 - +4.0 615,181 - +1.7 615,267 - +3.7 615,371 + Psyr_0544 0.10 -1.1 615,371 + Psyr_0544 0.10 -2.4 615,372 - Psyr_0544 0.11 +0.7 615,372 - Psyr_0544 0.11 +3.4 615,372 - Psyr_0544 0.11 +3.6 615,606 - Psyr_0544 0.23 +1.8 615,641 + Psyr_0544 0.25 -2.3 615,641 + Psyr_0544 0.25 +1.4 615,641 + Psyr_0544 0.25 +0.4 615,642 - Psyr_0544 0.25 +2.5 615,642 - Psyr_0544 0.25 +2.2 615,733 + Psyr_0544 0.30 -0.4
Or see this region's nucleotide sequence