Experiment: KB with Harmine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0512 and Psyr_0513 are separated by 226 nucleotides Psyr_0513 and Psyr_0514 overlap by 38 nucleotides Psyr_0514 and Psyr_0515 are separated by 137 nucleotides
Psyr_0512: Psyr_0512 - GCN5-related N-acetyltransferase, at 567,673 to 568,131
_0512
Psyr_0513: Psyr_0513 - amino acid ABC transporter substrate-binding protein, PAAT family, at 568,358 to 569,113
_0513
Psyr_0514: Psyr_0514 - Intergral membrane protein, YccS:Integral membrane protein, YccS/YhfK, at 569,076 to 571,274
_0514
Psyr_0515: Psyr_0515 - HI0933-like protein, at 571,412 to 572,590
_0515
Position (kb) 569
570
571
572 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 568.076 kb on + strand, within Psyr_0512 at 568.076 kb on + strand, within Psyr_0512 at 568.077 kb on - strand, within Psyr_0512 at 568.077 kb on - strand, within Psyr_0512 at 568.077 kb on - strand, within Psyr_0512 at 568.125 kb on + strand at 568.166 kb on + strand at 568.167 kb on - strand at 568.207 kb on + strand at 568.208 kb on - strand at 568.208 kb on - strand at 568.251 kb on + strand at 568.251 kb on + strand at 568.275 kb on + strand at 568.275 kb on + strand at 568.275 kb on + strand at 568.276 kb on - strand at 568.276 kb on - strand at 568.276 kb on - strand at 568.317 kb on - strand at 568.544 kb on + strand, within Psyr_0513 at 568.544 kb on + strand, within Psyr_0513 at 568.544 kb on + strand, within Psyr_0513 at 568.544 kb on + strand, within Psyr_0513 at 568.544 kb on + strand, within Psyr_0513 at 568.545 kb on - strand, within Psyr_0513 at 568.545 kb on - strand, within Psyr_0513 at 568.545 kb on - strand, within Psyr_0513 at 568.545 kb on - strand, within Psyr_0513 at 568.545 kb on - strand, within Psyr_0513 at 568.661 kb on + strand, within Psyr_0513 at 568.661 kb on + strand, within Psyr_0513 at 568.661 kb on + strand, within Psyr_0513 at 568.662 kb on - strand, within Psyr_0513 at 568.739 kb on + strand, within Psyr_0513 at 568.739 kb on + strand, within Psyr_0513 at 568.739 kb on + strand, within Psyr_0513 at 568.740 kb on - strand, within Psyr_0513 at 568.769 kb on + strand, within Psyr_0513 at 568.769 kb on + strand, within Psyr_0513 at 568.770 kb on - strand, within Psyr_0513 at 568.770 kb on - strand, within Psyr_0513 at 568.770 kb on - strand, within Psyr_0513 at 568.770 kb on - strand, within Psyr_0513 at 568.770 kb on - strand, within Psyr_0513 at 568.770 kb on - strand, within Psyr_0513 at 568.836 kb on + strand, within Psyr_0513 at 568.837 kb on - strand, within Psyr_0513 at 568.883 kb on + strand, within Psyr_0513 at 568.883 kb on + strand, within Psyr_0513 at 568.883 kb on + strand, within Psyr_0513 at 568.884 kb on - strand, within Psyr_0513 at 568.884 kb on - strand, within Psyr_0513 at 568.884 kb on - strand, within Psyr_0513 at 568.884 kb on - strand, within Psyr_0513 at 568.884 kb on - strand, within Psyr_0513 at 568.884 kb on - strand, within Psyr_0513 at 568.884 kb on - strand, within Psyr_0513 at 568.887 kb on + strand, within Psyr_0513 at 568.888 kb on - strand, within Psyr_0513 at 568.888 kb on - strand, within Psyr_0513 at 568.975 kb on + strand, within Psyr_0513 at 568.975 kb on + strand, within Psyr_0513 at 568.975 kb on + strand, within Psyr_0513 at 569.045 kb on - strand at 569.063 kb on + strand at 569.063 kb on + strand at 569.063 kb on + strand at 569.063 kb on + strand at 569.064 kb on - strand at 569.398 kb on + strand, within Psyr_0514 at 569.398 kb on + strand, within Psyr_0514 at 569.398 kb on + strand, within Psyr_0514 at 569.399 kb on - strand, within Psyr_0514 at 569.399 kb on - strand, within Psyr_0514 at 569.572 kb on + strand, within Psyr_0514 at 569.596 kb on + strand, within Psyr_0514 at 569.596 kb on + strand, within Psyr_0514 at 569.597 kb on - strand, within Psyr_0514 at 569.597 kb on - strand, within Psyr_0514 at 569.597 kb on - strand, within Psyr_0514 at 569.597 kb on - strand, within Psyr_0514 at 569.597 kb on - strand, within Psyr_0514 at 569.597 kb on - strand, within Psyr_0514 at 569.597 kb on - strand, within Psyr_0514 at 569.597 kb on - strand, within Psyr_0514 at 569.709 kb on - strand, within Psyr_0514 at 569.709 kb on - strand, within Psyr_0514 at 569.709 kb on - strand, within Psyr_0514 at 569.803 kb on + strand, within Psyr_0514 at 569.804 kb on - strand, within Psyr_0514 at 570.004 kb on + strand, within Psyr_0514 at 570.352 kb on + strand, within Psyr_0514 at 570.353 kb on - strand, within Psyr_0514 at 570.353 kb on - strand, within Psyr_0514 at 570.353 kb on - strand, within Psyr_0514 at 570.369 kb on + strand, within Psyr_0514 at 570.369 kb on + strand, within Psyr_0514 at 570.370 kb on - strand, within Psyr_0514 at 570.370 kb on - strand, within Psyr_0514 at 570.370 kb on - strand, within Psyr_0514 at 570.370 kb on - strand, within Psyr_0514 at 570.370 kb on - strand, within Psyr_0514 at 570.479 kb on - strand, within Psyr_0514 at 570.479 kb on - strand, within Psyr_0514 at 570.484 kb on + strand, within Psyr_0514 at 570.529 kb on + strand, within Psyr_0514 at 570.529 kb on + strand, within Psyr_0514 at 570.529 kb on + strand, within Psyr_0514 at 570.530 kb on - strand, within Psyr_0514 at 571.213 kb on + strand at 571.214 kb on - strand at 571.214 kb on - strand at 571.214 kb on - strand at 571.250 kb on + strand at 571.250 kb on + strand at 571.250 kb on + strand at 571.250 kb on + strand at 571.250 kb on + strand at 571.250 kb on + strand at 571.250 kb on + strand at 571.250 kb on + strand at 571.250 kb on + strand at 571.250 kb on + strand at 571.251 kb on - strand at 571.251 kb on - strand at 571.251 kb on - strand at 571.251 kb on - strand at 571.251 kb on - strand at 571.279 kb on + strand at 571.323 kb on + strand at 571.323 kb on + strand at 571.323 kb on + strand at 571.323 kb on + strand at 571.324 kb on - strand at 571.324 kb on - strand at 571.324 kb on - strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.348 kb on + strand at 571.348 kb on + strand at 571.348 kb on + strand at 571.348 kb on + strand at 571.348 kb on + strand at 571.348 kb on + strand at 571.349 kb on - strand at 571.349 kb on - strand at 571.368 kb on + strand at 571.370 kb on + strand at 571.398 kb on + strand at 571.398 kb on + strand at 571.398 kb on + strand at 571.419 kb on + strand at 571.419 kb on + strand at 571.419 kb on + strand at 571.419 kb on + strand at 571.419 kb on + strand at 571.420 kb on - strand at 571.420 kb on - strand at 571.420 kb on - strand at 571.420 kb on - strand at 571.420 kb on - strand at 571.420 kb on - strand at 571.420 kb on - strand at 571.434 kb on + strand at 571.434 kb on + strand at 571.435 kb on - strand at 571.509 kb on + strand at 571.509 kb on + strand at 571.510 kb on - strand at 571.672 kb on - strand, within Psyr_0515 at 571.672 kb on - strand, within Psyr_0515 at 571.672 kb on - strand, within Psyr_0515
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmine 10 mM remove 568,076 + Psyr_0512 0.88 +0.0 568,076 + Psyr_0512 0.88 -0.8 568,077 - Psyr_0512 0.88 -1.2 568,077 - Psyr_0512 0.88 +0.8 568,077 - Psyr_0512 0.88 -0.6 568,125 + +1.4 568,166 + +0.8 568,167 - -2.6 568,207 + -0.6 568,208 - +0.2 568,208 - -0.5 568,251 + -0.4 568,251 + -0.9 568,275 + -4.3 568,275 + +1.0 568,275 + +0.1 568,276 - +0.4 568,276 - +0.3 568,276 - -0.2 568,317 - +0.8 568,544 + Psyr_0513 0.25 +0.4 568,544 + Psyr_0513 0.25 +0.3 568,544 + Psyr_0513 0.25 +0.8 568,544 + Psyr_0513 0.25 -1.2 568,544 + Psyr_0513 0.25 -0.6 568,545 - Psyr_0513 0.25 +0.3 568,545 - Psyr_0513 0.25 -1.7 568,545 - Psyr_0513 0.25 -0.6 568,545 - Psyr_0513 0.25 -0.2 568,545 - Psyr_0513 0.25 -0.4 568,661 + Psyr_0513 0.40 +1.1 568,661 + Psyr_0513 0.40 +0.1 568,661 + Psyr_0513 0.40 +0.8 568,662 - Psyr_0513 0.40 -0.4 568,739 + Psyr_0513 0.50 -0.2 568,739 + Psyr_0513 0.50 +0.7 568,739 + Psyr_0513 0.50 +1.0 568,740 - Psyr_0513 0.51 +0.0 568,769 + Psyr_0513 0.54 -0.4 568,769 + Psyr_0513 0.54 -0.5 568,770 - Psyr_0513 0.54 -0.5 568,770 - Psyr_0513 0.54 +0.1 568,770 - Psyr_0513 0.54 +0.1 568,770 - Psyr_0513 0.54 +0.2 568,770 - Psyr_0513 0.54 -1.1 568,770 - Psyr_0513 0.54 -1.1 568,836 + Psyr_0513 0.63 +0.8 568,837 - Psyr_0513 0.63 -0.4 568,883 + Psyr_0513 0.69 +0.2 568,883 + Psyr_0513 0.69 +0.6 568,883 + Psyr_0513 0.69 +0.4 568,884 - Psyr_0513 0.70 +1.4 568,884 - Psyr_0513 0.70 -0.1 568,884 - Psyr_0513 0.70 -0.7 568,884 - Psyr_0513 0.70 +0.2 568,884 - Psyr_0513 0.70 +0.2 568,884 - Psyr_0513 0.70 +0.4 568,884 - Psyr_0513 0.70 +0.3 568,887 + Psyr_0513 0.70 -0.4 568,888 - Psyr_0513 0.70 -0.1 568,888 - Psyr_0513 0.70 +0.6 568,975 + Psyr_0513 0.82 -1.3 568,975 + Psyr_0513 0.82 -0.1 568,975 + Psyr_0513 0.82 -0.4 569,045 - +0.0 569,063 + -0.8 569,063 + +0.0 569,063 + +0.7 569,063 + +0.8 569,064 - -0.6 569,398 + Psyr_0514 0.15 +1.7 569,398 + Psyr_0514 0.15 +0.8 569,398 + Psyr_0514 0.15 +0.8 569,399 - Psyr_0514 0.15 -0.4 569,399 - Psyr_0514 0.15 -0.5 569,572 + Psyr_0514 0.23 +0.6 569,596 + Psyr_0514 0.24 +0.4 569,596 + Psyr_0514 0.24 +0.4 569,597 - Psyr_0514 0.24 -1.3 569,597 - Psyr_0514 0.24 +0.1 569,597 - Psyr_0514 0.24 +0.3 569,597 - Psyr_0514 0.24 -0.0 569,597 - Psyr_0514 0.24 +0.2 569,597 - Psyr_0514 0.24 +0.9 569,597 - Psyr_0514 0.24 -0.2 569,597 - Psyr_0514 0.24 +1.5 569,709 - Psyr_0514 0.29 +1.2 569,709 - Psyr_0514 0.29 +1.3 569,709 - Psyr_0514 0.29 +0.8 569,803 + Psyr_0514 0.33 +0.9 569,804 - Psyr_0514 0.33 +0.8 570,004 + Psyr_0514 0.42 +0.3 570,352 + Psyr_0514 0.58 +1.9 570,353 - Psyr_0514 0.58 -0.3 570,353 - Psyr_0514 0.58 -0.9 570,353 - Psyr_0514 0.58 -1.0 570,369 + Psyr_0514 0.59 -0.4 570,369 + Psyr_0514 0.59 -0.8 570,370 - Psyr_0514 0.59 +0.0 570,370 - Psyr_0514 0.59 -1.4 570,370 - Psyr_0514 0.59 -0.2 570,370 - Psyr_0514 0.59 -0.4 570,370 - Psyr_0514 0.59 -0.4 570,479 - Psyr_0514 0.64 -0.2 570,479 - Psyr_0514 0.64 +3.4 570,484 + Psyr_0514 0.64 +2.2 570,529 + Psyr_0514 0.66 +1.3 570,529 + Psyr_0514 0.66 +0.4 570,529 + Psyr_0514 0.66 +0.7 570,530 - Psyr_0514 0.66 +0.2 571,213 + -0.3 571,214 - -0.6 571,214 - +0.2 571,214 - -0.7 571,250 + -0.7 571,250 + +1.4 571,250 + +0.2 571,250 + -1.4 571,250 + -0.6 571,250 + +0.1 571,250 + +0.3 571,250 + +1.5 571,250 + +2.8 571,250 + +0.8 571,251 - -1.6 571,251 - -0.9 571,251 - -0.2 571,251 - -1.3 571,251 - -0.4 571,279 + -0.2 571,323 + +0.3 571,323 + +0.4 571,323 + +1.0 571,323 + +0.9 571,324 - -1.9 571,324 - -1.3 571,324 - -2.3 571,327 + -0.7 571,327 + +0.4 571,327 + +0.0 571,327 + +0.6 571,327 + -1.0 571,327 + +0.1 571,327 + +1.4 571,327 + +1.0 571,327 + +0.8 571,327 + -0.7 571,327 + +2.1 571,327 + +0.5 571,327 + -1.7 571,327 + -2.6 571,328 - -0.0 571,328 - -1.6 571,328 - -0.2 571,328 - -1.0 571,328 - +1.4 571,328 - +0.3 571,348 + -0.2 571,348 + -0.3 571,348 + -0.2 571,348 + +0.0 571,348 + +0.7 571,348 + -0.5 571,349 - +0.8 571,349 - -1.4 571,368 + +0.7 571,370 + +0.9 571,398 + +0.6 571,398 + -0.5 571,398 + -0.2 571,419 + +1.5 571,419 + +0.6 571,419 + -0.0 571,419 + +0.5 571,419 + +1.1 571,420 - -1.5 571,420 - -2.2 571,420 - +0.2 571,420 - -0.3 571,420 - -0.3 571,420 - +0.3 571,420 - +1.1 571,434 + -0.6 571,434 + -0.1 571,435 - +1.0 571,509 + +0.9 571,509 + +0.4 571,510 - -0.2 571,672 - Psyr_0515 0.22 +0.2 571,672 - Psyr_0515 0.22 -0.7 571,672 - Psyr_0515 0.22 -0.6
Or see this region's nucleotide sequence