Experiment: KB with Harmine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0118 and Psyr_0119 are separated by 84 nucleotides Psyr_0119 and Psyr_0120 are separated by 343 nucleotides Psyr_0120 and Psyr_0121 are separated by 81 nucleotides Psyr_0121 and Psyr_0122 are separated by 89 nucleotides
Psyr_0118: Psyr_0118 - hypothetical protein, at 128,434 to 129,486
_0118
Psyr_0119: Psyr_0119 - CDS, at 129,571 to 129,822
_0119
Psyr_0120: Psyr_0120 - Short-chain dehydrogenase/reductase SDR, at 130,166 to 130,936
_0120
Psyr_0121: Psyr_0121 - transcriptional regulator, TetR family, at 131,018 to 131,647
_0121
Psyr_0122: Psyr_0122 - NAD(P)H dehydrogenase (quinone), at 131,737 to 132,393
_0122
Position (kb) 130
131 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 129.176 kb on + strand, within Psyr_0118 at 129.176 kb on + strand, within Psyr_0118 at 129.176 kb on + strand, within Psyr_0118 at 129.176 kb on + strand, within Psyr_0118 at 129.176 kb on + strand, within Psyr_0118 at 129.176 kb on + strand, within Psyr_0118 at 129.176 kb on + strand, within Psyr_0118 at 129.177 kb on - strand, within Psyr_0118 at 129.177 kb on - strand, within Psyr_0118 at 129.177 kb on - strand, within Psyr_0118 at 129.233 kb on - strand, within Psyr_0118 at 129.266 kb on + strand, within Psyr_0118 at 129.274 kb on + strand, within Psyr_0118 at 129.275 kb on - strand, within Psyr_0118 at 129.284 kb on - strand, within Psyr_0118 at 129.284 kb on - strand, within Psyr_0118 at 129.284 kb on - strand, within Psyr_0118 at 129.284 kb on - strand, within Psyr_0118 at 129.284 kb on - strand, within Psyr_0118 at 129.429 kb on + strand at 129.433 kb on + strand at 129.434 kb on - strand at 129.434 kb on - strand at 129.515 kb on + strand at 129.547 kb on + strand at 129.619 kb on + strand, within Psyr_0119 at 129.619 kb on + strand, within Psyr_0119 at 129.620 kb on - strand, within Psyr_0119 at 129.623 kb on + strand, within Psyr_0119 at 129.623 kb on + strand, within Psyr_0119 at 129.623 kb on + strand, within Psyr_0119 at 129.624 kb on - strand, within Psyr_0119 at 129.624 kb on - strand, within Psyr_0119 at 129.624 kb on - strand, within Psyr_0119 at 129.624 kb on - strand, within Psyr_0119 at 129.624 kb on - strand, within Psyr_0119 at 129.640 kb on + strand, within Psyr_0119 at 129.641 kb on - strand, within Psyr_0119 at 129.715 kb on + strand, within Psyr_0119 at 129.715 kb on + strand, within Psyr_0119 at 129.716 kb on - strand, within Psyr_0119 at 129.839 kb on + strand at 129.840 kb on - strand at 129.847 kb on + strand at 129.938 kb on - strand at 129.939 kb on + strand at 129.939 kb on + strand at 129.939 kb on + strand at 129.955 kb on + strand at 130.000 kb on + strand at 130.000 kb on + strand at 130.000 kb on + strand at 130.000 kb on + strand at 130.001 kb on - strand at 130.001 kb on - strand at 130.001 kb on - strand at 130.001 kb on - strand at 130.110 kb on + strand at 130.110 kb on + strand at 130.110 kb on + strand at 130.110 kb on + strand at 130.110 kb on + strand at 130.111 kb on - strand at 130.111 kb on - strand at 130.111 kb on - strand at 130.111 kb on - strand at 130.111 kb on - strand at 130.166 kb on - strand at 130.281 kb on + strand, within Psyr_0120 at 130.340 kb on + strand, within Psyr_0120 at 130.341 kb on - strand, within Psyr_0120 at 130.451 kb on - strand, within Psyr_0120 at 130.451 kb on - strand, within Psyr_0120 at 130.455 kb on + strand, within Psyr_0120 at 130.455 kb on + strand, within Psyr_0120 at 130.456 kb on - strand, within Psyr_0120 at 130.456 kb on - strand, within Psyr_0120 at 130.456 kb on - strand, within Psyr_0120 at 130.456 kb on - strand, within Psyr_0120 at 130.459 kb on + strand, within Psyr_0120 at 130.459 kb on + strand, within Psyr_0120 at 130.460 kb on - strand, within Psyr_0120 at 130.460 kb on - strand, within Psyr_0120 at 130.575 kb on + strand, within Psyr_0120 at 130.575 kb on + strand, within Psyr_0120 at 130.575 kb on + strand, within Psyr_0120 at 130.576 kb on - strand, within Psyr_0120 at 130.576 kb on - strand, within Psyr_0120 at 130.576 kb on - strand, within Psyr_0120 at 130.576 kb on - strand, within Psyr_0120 at 130.636 kb on + strand, within Psyr_0120 at 130.636 kb on + strand, within Psyr_0120 at 130.664 kb on + strand, within Psyr_0120 at 130.670 kb on + strand, within Psyr_0120 at 130.670 kb on + strand, within Psyr_0120 at 130.670 kb on + strand, within Psyr_0120 at 130.670 kb on + strand, within Psyr_0120 at 130.671 kb on - strand, within Psyr_0120 at 130.671 kb on - strand, within Psyr_0120 at 130.671 kb on - strand, within Psyr_0120 at 130.747 kb on + strand, within Psyr_0120 at 130.747 kb on + strand, within Psyr_0120 at 130.747 kb on + strand, within Psyr_0120 at 130.747 kb on + strand, within Psyr_0120 at 130.747 kb on + strand, within Psyr_0120 at 130.747 kb on + strand, within Psyr_0120 at 130.747 kb on + strand, within Psyr_0120 at 130.761 kb on + strand, within Psyr_0120 at 130.761 kb on + strand, within Psyr_0120 at 130.761 kb on + strand, within Psyr_0120 at 130.762 kb on - strand, within Psyr_0120 at 130.762 kb on - strand, within Psyr_0120 at 130.869 kb on + strand at 130.967 kb on + strand at 130.968 kb on - strand at 131.147 kb on + strand, within Psyr_0121 at 131.147 kb on + strand, within Psyr_0121 at 131.147 kb on + strand, within Psyr_0121 at 131.147 kb on + strand, within Psyr_0121 at 131.147 kb on + strand, within Psyr_0121 at 131.148 kb on - strand, within Psyr_0121 at 131.148 kb on - strand, within Psyr_0121 at 131.148 kb on - strand, within Psyr_0121 at 131.148 kb on - strand, within Psyr_0121 at 131.230 kb on + strand, within Psyr_0121 at 131.230 kb on + strand, within Psyr_0121 at 131.231 kb on - strand, within Psyr_0121 at 131.231 kb on - strand, within Psyr_0121 at 131.231 kb on - strand, within Psyr_0121 at 131.239 kb on - strand, within Psyr_0121 at 131.319 kb on - strand, within Psyr_0121 at 131.331 kb on + strand, within Psyr_0121 at 131.331 kb on + strand, within Psyr_0121 at 131.331 kb on + strand, within Psyr_0121 at 131.331 kb on + strand, within Psyr_0121 at 131.331 kb on + strand, within Psyr_0121 at 131.331 kb on + strand, within Psyr_0121 at 131.331 kb on + strand, within Psyr_0121 at 131.331 kb on + strand, within Psyr_0121 at 131.332 kb on - strand, within Psyr_0121 at 131.332 kb on - strand, within Psyr_0121 at 131.332 kb on - strand, within Psyr_0121 at 131.332 kb on - strand, within Psyr_0121 at 131.332 kb on - strand, within Psyr_0121 at 131.332 kb on - strand, within Psyr_0121 at 131.332 kb on - strand, within Psyr_0121 at 131.332 kb on - strand, within Psyr_0121 at 131.332 kb on - strand, within Psyr_0121 at 131.342 kb on + strand, within Psyr_0121 at 131.343 kb on - strand, within Psyr_0121 at 131.354 kb on + strand, within Psyr_0121 at 131.430 kb on + strand, within Psyr_0121 at 131.430 kb on + strand, within Psyr_0121 at 131.430 kb on + strand, within Psyr_0121 at 131.430 kb on + strand, within Psyr_0121 at 131.431 kb on - strand, within Psyr_0121 at 131.431 kb on - strand, within Psyr_0121 at 131.431 kb on - strand, within Psyr_0121 at 131.431 kb on - strand, within Psyr_0121 at 131.472 kb on + strand, within Psyr_0121 at 131.472 kb on + strand, within Psyr_0121 at 131.473 kb on - strand, within Psyr_0121 at 131.651 kb on + strand at 131.652 kb on - strand at 131.654 kb on - strand at 131.656 kb on - strand at 131.656 kb on - strand at 131.664 kb on + strand at 131.735 kb on + strand at 131.735 kb on + strand at 131.735 kb on + strand at 131.735 kb on + strand at 131.736 kb on - strand at 131.819 kb on + strand, within Psyr_0122 at 131.819 kb on + strand, within Psyr_0122 at 131.819 kb on + strand, within Psyr_0122 at 131.819 kb on + strand, within Psyr_0122 at 131.819 kb on + strand, within Psyr_0122 at 131.819 kb on + strand, within Psyr_0122 at 131.819 kb on + strand, within Psyr_0122 at 131.820 kb on - strand, within Psyr_0122 at 131.820 kb on - strand, within Psyr_0122 at 131.820 kb on - strand, within Psyr_0122 at 131.820 kb on - strand, within Psyr_0122 at 131.820 kb on - strand, within Psyr_0122 at 131.820 kb on - strand, within Psyr_0122 at 131.820 kb on - strand, within Psyr_0122 at 131.822 kb on + strand, within Psyr_0122 at 131.822 kb on + strand, within Psyr_0122 at 131.822 kb on + strand, within Psyr_0122 at 131.823 kb on - strand, within Psyr_0122 at 131.823 kb on - strand, within Psyr_0122 at 131.823 kb on - strand, within Psyr_0122 at 131.823 kb on - strand, within Psyr_0122 at 131.823 kb on - strand, within Psyr_0122 at 131.823 kb on - strand, within Psyr_0122 at 131.843 kb on + strand, within Psyr_0122 at 131.906 kb on + strand, within Psyr_0122 at 131.906 kb on + strand, within Psyr_0122 at 131.906 kb on + strand, within Psyr_0122 at 131.906 kb on + strand, within Psyr_0122 at 131.906 kb on + strand, within Psyr_0122 at 131.906 kb on + strand, within Psyr_0122 at 131.906 kb on + strand, within Psyr_0122 at 131.907 kb on - strand, within Psyr_0122 at 131.907 kb on - strand, within Psyr_0122 at 131.907 kb on - strand, within Psyr_0122 at 131.907 kb on - strand, within Psyr_0122 at 131.907 kb on - strand, within Psyr_0122 at 131.907 kb on - strand, within Psyr_0122
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmine 10 mM remove 129,176 + Psyr_0118 0.70 +0.1 129,176 + Psyr_0118 0.70 +1.0 129,176 + Psyr_0118 0.70 +0.5 129,176 + Psyr_0118 0.70 +0.2 129,176 + Psyr_0118 0.70 +0.0 129,176 + Psyr_0118 0.70 +0.0 129,176 + Psyr_0118 0.70 -0.5 129,177 - Psyr_0118 0.71 -0.2 129,177 - Psyr_0118 0.71 -2.6 129,177 - Psyr_0118 0.71 -1.9 129,233 - Psyr_0118 0.76 -0.3 129,266 + Psyr_0118 0.79 -1.1 129,274 + Psyr_0118 0.80 -0.5 129,275 - Psyr_0118 0.80 -0.1 129,284 - Psyr_0118 0.81 +0.2 129,284 - Psyr_0118 0.81 +0.1 129,284 - Psyr_0118 0.81 -0.0 129,284 - Psyr_0118 0.81 +0.8 129,284 - Psyr_0118 0.81 +0.1 129,429 + +0.0 129,433 + +1.0 129,434 - -1.4 129,434 - -2.4 129,515 + -0.7 129,547 + +1.4 129,619 + Psyr_0119 0.19 -0.7 129,619 + Psyr_0119 0.19 -0.3 129,620 - Psyr_0119 0.19 -0.9 129,623 + Psyr_0119 0.21 -0.6 129,623 + Psyr_0119 0.21 +0.9 129,623 + Psyr_0119 0.21 -0.9 129,624 - Psyr_0119 0.21 +0.3 129,624 - Psyr_0119 0.21 -0.3 129,624 - Psyr_0119 0.21 -2.7 129,624 - Psyr_0119 0.21 -1.1 129,624 - Psyr_0119 0.21 -1.2 129,640 + Psyr_0119 0.27 -0.5 129,641 - Psyr_0119 0.28 -0.5 129,715 + Psyr_0119 0.57 -0.9 129,715 + Psyr_0119 0.57 -0.2 129,716 - Psyr_0119 0.58 +0.5 129,839 + -1.8 129,840 - +0.6 129,847 + +0.0 129,938 - +0.3 129,939 + -0.7 129,939 + +1.1 129,939 + +0.9 129,955 + -0.5 130,000 + -1.1 130,000 + -1.0 130,000 + +0.4 130,000 + +0.4 130,001 - +1.1 130,001 - -1.9 130,001 - +0.2 130,001 - +1.7 130,110 + +0.6 130,110 + +0.1 130,110 + +1.0 130,110 + -0.3 130,110 + -0.0 130,111 - +1.5 130,111 - -0.7 130,111 - -0.2 130,111 - +0.5 130,111 - -0.4 130,166 - -1.5 130,281 + Psyr_0120 0.15 -0.9 130,340 + Psyr_0120 0.23 +0.1 130,341 - Psyr_0120 0.23 +1.9 130,451 - Psyr_0120 0.37 -1.1 130,451 - Psyr_0120 0.37 +0.9 130,455 + Psyr_0120 0.37 -0.5 130,455 + Psyr_0120 0.37 -1.1 130,456 - Psyr_0120 0.38 -1.2 130,456 - Psyr_0120 0.38 +0.2 130,456 - Psyr_0120 0.38 +1.5 130,456 - Psyr_0120 0.38 +0.5 130,459 + Psyr_0120 0.38 -0.4 130,459 + Psyr_0120 0.38 +0.0 130,460 - Psyr_0120 0.38 -0.5 130,460 - Psyr_0120 0.38 -0.5 130,575 + Psyr_0120 0.53 +2.5 130,575 + Psyr_0120 0.53 +1.1 130,575 + Psyr_0120 0.53 +0.4 130,576 - Psyr_0120 0.53 -0.4 130,576 - Psyr_0120 0.53 +1.7 130,576 - Psyr_0120 0.53 +1.5 130,576 - Psyr_0120 0.53 +1.1 130,636 + Psyr_0120 0.61 +0.8 130,636 + Psyr_0120 0.61 -1.1 130,664 + Psyr_0120 0.65 +0.1 130,670 + Psyr_0120 0.65 -0.8 130,670 + Psyr_0120 0.65 -1.2 130,670 + Psyr_0120 0.65 -0.1 130,670 + Psyr_0120 0.65 -1.4 130,671 - Psyr_0120 0.65 -2.5 130,671 - Psyr_0120 0.65 +0.4 130,671 - Psyr_0120 0.65 -1.5 130,747 + Psyr_0120 0.75 +0.6 130,747 + Psyr_0120 0.75 -1.1 130,747 + Psyr_0120 0.75 -0.6 130,747 + Psyr_0120 0.75 +0.5 130,747 + Psyr_0120 0.75 -0.5 130,747 + Psyr_0120 0.75 -0.3 130,747 + Psyr_0120 0.75 +0.2 130,761 + Psyr_0120 0.77 +1.9 130,761 + Psyr_0120 0.77 +0.8 130,761 + Psyr_0120 0.77 +0.4 130,762 - Psyr_0120 0.77 -0.5 130,762 - Psyr_0120 0.77 +1.3 130,869 + -0.1 130,967 + -1.4 130,968 - +0.9 131,147 + Psyr_0121 0.20 -0.1 131,147 + Psyr_0121 0.20 -0.9 131,147 + Psyr_0121 0.20 +0.2 131,147 + Psyr_0121 0.20 -0.4 131,147 + Psyr_0121 0.20 -0.8 131,148 - Psyr_0121 0.21 +0.1 131,148 - Psyr_0121 0.21 -0.4 131,148 - Psyr_0121 0.21 +0.6 131,148 - Psyr_0121 0.21 -0.8 131,230 + Psyr_0121 0.34 -1.2 131,230 + Psyr_0121 0.34 -1.1 131,231 - Psyr_0121 0.34 -1.5 131,231 - Psyr_0121 0.34 +0.6 131,231 - Psyr_0121 0.34 +0.2 131,239 - Psyr_0121 0.35 -1.9 131,319 - Psyr_0121 0.48 -0.4 131,331 + Psyr_0121 0.50 -2.9 131,331 + Psyr_0121 0.50 +0.3 131,331 + Psyr_0121 0.50 +0.1 131,331 + Psyr_0121 0.50 -0.4 131,331 + Psyr_0121 0.50 +1.0 131,331 + Psyr_0121 0.50 +0.9 131,331 + Psyr_0121 0.50 -1.4 131,331 + Psyr_0121 0.50 -0.7 131,332 - Psyr_0121 0.50 -1.7 131,332 - Psyr_0121 0.50 +0.2 131,332 - Psyr_0121 0.50 +0.2 131,332 - Psyr_0121 0.50 -2.2 131,332 - Psyr_0121 0.50 +0.3 131,332 - Psyr_0121 0.50 +0.4 131,332 - Psyr_0121 0.50 -0.2 131,332 - Psyr_0121 0.50 +0.7 131,332 - Psyr_0121 0.50 +0.2 131,342 + Psyr_0121 0.51 +0.4 131,343 - Psyr_0121 0.52 +1.9 131,354 + Psyr_0121 0.53 +1.5 131,430 + Psyr_0121 0.65 +0.5 131,430 + Psyr_0121 0.65 +0.0 131,430 + Psyr_0121 0.65 -2.6 131,430 + Psyr_0121 0.65 +0.3 131,431 - Psyr_0121 0.66 +0.6 131,431 - Psyr_0121 0.66 -1.2 131,431 - Psyr_0121 0.66 +0.1 131,431 - Psyr_0121 0.66 -0.2 131,472 + Psyr_0121 0.72 +1.5 131,472 + Psyr_0121 0.72 +0.1 131,473 - Psyr_0121 0.72 -1.5 131,651 + -0.1 131,652 - +0.2 131,654 - +1.2 131,656 - -0.3 131,656 - +0.0 131,664 + -0.2 131,735 + +0.5 131,735 + -0.4 131,735 + -2.8 131,735 + +0.8 131,736 - -0.1 131,819 + Psyr_0122 0.12 -0.3 131,819 + Psyr_0122 0.12 -0.1 131,819 + Psyr_0122 0.12 -0.5 131,819 + Psyr_0122 0.12 -2.2 131,819 + Psyr_0122 0.12 +0.3 131,819 + Psyr_0122 0.12 -0.1 131,819 + Psyr_0122 0.12 +0.9 131,820 - Psyr_0122 0.13 -1.9 131,820 - Psyr_0122 0.13 +0.6 131,820 - Psyr_0122 0.13 -0.5 131,820 - Psyr_0122 0.13 -1.1 131,820 - Psyr_0122 0.13 -3.6 131,820 - Psyr_0122 0.13 -0.3 131,820 - Psyr_0122 0.13 -0.4 131,822 + Psyr_0122 0.13 +0.1 131,822 + Psyr_0122 0.13 -1.4 131,822 + Psyr_0122 0.13 -1.4 131,823 - Psyr_0122 0.13 -1.1 131,823 - Psyr_0122 0.13 -0.7 131,823 - Psyr_0122 0.13 -1.2 131,823 - Psyr_0122 0.13 -0.5 131,823 - Psyr_0122 0.13 +0.0 131,823 - Psyr_0122 0.13 -0.0 131,843 + Psyr_0122 0.16 -0.4 131,906 + Psyr_0122 0.26 -0.5 131,906 + Psyr_0122 0.26 +0.4 131,906 + Psyr_0122 0.26 -1.3 131,906 + Psyr_0122 0.26 -0.6 131,906 + Psyr_0122 0.26 +1.1 131,906 + Psyr_0122 0.26 +0.3 131,906 + Psyr_0122 0.26 -1.9 131,907 - Psyr_0122 0.26 -0.0 131,907 - Psyr_0122 0.26 +1.9 131,907 - Psyr_0122 0.26 -0.0 131,907 - Psyr_0122 0.26 +1.5 131,907 - Psyr_0122 0.26 +0.7 131,907 - Psyr_0122 0.26 +0.3
Or see this region's nucleotide sequence