Strain Fitness in Escherichia coli BW25113 around b4494

Experiment: Trimethoprim 0.0025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthrS and arpB are separated by 523 nucleotidesarpB and yniD are separated by 171 nucleotidesyniD and ydiY are separated by 52 nucleotides b1719: thrS - threonyl-tRNA synthetase (NCBI), at 1,798,666 to 1,800,594 thrS b4494: arpB - no description, at 1,801,118 to 1,803,017 arpB b4535: yniD - predicted protein (RefSeq), at 1,803,189 to 1,803,296 yniD b1722: ydiY - hypothetical protein (NCBI), at 1,803,349 to 1,804,107 ydiY Position (kb) 1801 1802 1803 1804Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 1800.838 kb on - strandat 1800.879 kb on - strandat 1800.891 kb on + strandat 1800.899 kb on - strandat 1800.906 kb on + strandat 1800.906 kb on + strandat 1800.906 kb on + strandat 1800.958 kb on + strandat 1800.995 kb on + strandat 1800.995 kb on + strandat 1800.995 kb on + strandat 1801.168 kb on - strandat 1801.177 kb on + strandat 1801.615 kb on - strand, within arpBat 1801.743 kb on + strand, within arpBat 1801.781 kb on + strand, within arpBat 1801.790 kb on + strand, within arpBat 1801.795 kb on + strand, within arpBat 1801.795 kb on + strand, within arpBat 1801.859 kb on + strand, within arpBat 1802.045 kb on + strand, within arpBat 1802.064 kb on + strand, within arpBat 1802.069 kb on + strand, within arpBat 1802.069 kb on + strand, within arpBat 1802.088 kb on + strand, within arpBat 1802.088 kb on + strand, within arpBat 1802.179 kb on + strand, within arpBat 1802.182 kb on + strand, within arpBat 1802.201 kb on + strand, within arpBat 1802.599 kb on - strand, within arpBat 1803.076 kb on - strandat 1803.076 kb on - strandat 1803.089 kb on - strandat 1803.091 kb on - strandat 1803.093 kb on - strandat 1803.139 kb on - strandat 1803.171 kb on - strandat 1803.189 kb on - strandat 1803.192 kb on - strandat 1803.192 kb on - strandat 1803.192 kb on - strandat 1803.232 kb on + strand, within yniDat 1803.236 kb on - strand, within yniDat 1803.261 kb on - strand, within yniDat 1803.261 kb on - strand, within yniDat 1803.279 kb on + strand, within yniDat 1803.483 kb on + strand, within ydiYat 1803.550 kb on + strand, within ydiYat 1803.588 kb on - strand, within ydiYat 1803.589 kb on - strand, within ydiYat 1803.613 kb on + strand, within ydiYat 1803.642 kb on - strand, within ydiYat 1803.644 kb on - strand, within ydiYat 1803.669 kb on - strand, within ydiYat 1803.672 kb on - strand, within ydiYat 1803.672 kb on - strand, within ydiYat 1803.870 kb on - strand, within ydiYat 1803.889 kb on - strand, within ydiYat 1803.889 kb on - strand, within ydiYat 1803.889 kb on - strand, within ydiYat 1803.889 kb on - strand, within ydiYat 1803.896 kb on - strand, within ydiYat 1803.938 kb on + strand, within ydiYat 1803.997 kb on + strand, within ydiYat 1803.997 kb on + strand, within ydiYat 1804.005 kb on - strand, within ydiYat 1804.005 kb on - strand, within ydiYat 1804.006 kb on + strand, within ydiYat 1804.014 kb on - strand, within ydiYat 1804.014 kb on - strand, within ydiY

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Per-strain Table

Position Strand Gene LocusTag Fraction Trimethoprim 0.0025 mM
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1,800,838 - +0.2
1,800,879 - -0.6
1,800,891 + +0.6
1,800,899 - +2.2
1,800,906 + +0.4
1,800,906 + +1.8
1,800,906 + -0.7
1,800,958 + -1.3
1,800,995 + +0.6
1,800,995 + +2.2
1,800,995 + -0.3
1,801,168 - +2.2
1,801,177 + +2.2
1,801,615 - arpB b4494 0.26 +2.2
1,801,743 + arpB b4494 0.33 +2.2
1,801,781 + arpB b4494 0.35 +1.8
1,801,790 + arpB b4494 0.35 +3.8
1,801,795 + arpB b4494 0.36 +1.2
1,801,795 + arpB b4494 0.36 +0.6
1,801,859 + arpB b4494 0.39 +2.2
1,802,045 + arpB b4494 0.49 -0.6
1,802,064 + arpB b4494 0.50 +2.2
1,802,069 + arpB b4494 0.50 +2.2
1,802,069 + arpB b4494 0.50 -0.4
1,802,088 + arpB b4494 0.51 +2.2
1,802,088 + arpB b4494 0.51 +1.6
1,802,179 + arpB b4494 0.56 +2.2
1,802,182 + arpB b4494 0.56 +2.2
1,802,201 + arpB b4494 0.57 +0.2
1,802,599 - arpB b4494 0.78 +0.6
1,803,076 - -1.1
1,803,076 - -1.1
1,803,089 - +0.3
1,803,091 - +1.2
1,803,093 - +2.8
1,803,139 - +3.2
1,803,171 - +1.5
1,803,189 - -0.8
1,803,192 - -2.0
1,803,192 - -0.2
1,803,192 - -0.6
1,803,232 + yniD b4535 0.40 +0.6
1,803,236 - yniD b4535 0.44 +2.2
1,803,261 - yniD b4535 0.67 +0.2
1,803,261 - yniD b4535 0.67 +1.2
1,803,279 + yniD b4535 0.83 +1.5
1,803,483 + ydiY b1722 0.18 +0.6
1,803,550 + ydiY b1722 0.26 -0.7
1,803,588 - ydiY b1722 0.31 -0.4
1,803,589 - ydiY b1722 0.32 -0.2
1,803,613 + ydiY b1722 0.35 +1.1
1,803,642 - ydiY b1722 0.39 +0.1
1,803,644 - ydiY b1722 0.39 +0.8
1,803,669 - ydiY b1722 0.42 +0.0
1,803,672 - ydiY b1722 0.43 -1.5
1,803,672 - ydiY b1722 0.43 +1.3
1,803,870 - ydiY b1722 0.69 +0.5
1,803,889 - ydiY b1722 0.71 -0.5
1,803,889 - ydiY b1722 0.71 -0.2
1,803,889 - ydiY b1722 0.71 +0.6
1,803,889 - ydiY b1722 0.71 -0.4
1,803,896 - ydiY b1722 0.72 -2.0
1,803,938 + ydiY b1722 0.78 -1.3
1,803,997 + ydiY b1722 0.85 +0.5
1,803,997 + ydiY b1722 0.85 +0.2
1,804,005 - ydiY b1722 0.86 +1.2
1,804,005 - ydiY b1722 0.86 +2.2
1,804,006 + ydiY b1722 0.87 +2.2
1,804,014 - ydiY b1722 0.88 +1.2
1,804,014 - ydiY b1722 0.88 +0.4

Or see this region's nucleotide sequence