Experiment: Trimethoprim 0.0025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fabR and yijD overlap by 1 nucleotides yijD and trmA are separated by 39 nucleotides trmA and btuB are separated by 368 nucleotides
b3963: fabR - DNA-binding transcriptional repressor (RefSeq), at 4,159,147 to 4,159,794
fabR
b3964: yijD - conserved inner membrane protein (NCBI), at 4,159,794 to 4,160,153
yijD
b3965: trmA - tRNA (uracil-5-)-methyltransferase (NCBI), at 4,160,193 to 4,161,293
trmA
b3966: btuB - vitamin B12/cobalamin outer membrane transporter (NCBI), at 4,161,662 to 4,163,506
btuB
Position (kb)
4160
4161
4162 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4159.215 kb on - strand, within fabR at 4159.220 kb on - strand, within fabR at 4159.220 kb on - strand, within fabR at 4159.237 kb on - strand, within fabR at 4159.237 kb on - strand, within fabR at 4159.238 kb on + strand, within fabR at 4159.238 kb on + strand, within fabR at 4159.278 kb on - strand, within fabR at 4159.291 kb on - strand, within fabR at 4159.291 kb on - strand, within fabR at 4159.321 kb on + strand, within fabR at 4159.351 kb on + strand, within fabR at 4159.352 kb on + strand, within fabR at 4159.354 kb on - strand, within fabR at 4159.356 kb on + strand, within fabR at 4159.402 kb on + strand, within fabR at 4159.402 kb on + strand, within fabR at 4159.403 kb on + strand, within fabR at 4159.424 kb on + strand, within fabR at 4159.445 kb on + strand, within fabR at 4159.559 kb on + strand, within fabR at 4159.559 kb on + strand, within fabR at 4159.629 kb on + strand, within fabR at 4159.643 kb on - strand, within fabR at 4159.656 kb on - strand, within fabR at 4159.697 kb on + strand, within fabR at 4159.697 kb on + strand, within fabR at 4159.699 kb on - strand, within fabR at 4159.699 kb on - strand, within fabR at 4159.726 kb on + strand, within fabR at 4159.726 kb on + strand, within fabR at 4159.734 kb on - strand at 4159.789 kb on + strand at 4159.799 kb on - strand at 4159.811 kb on + strand at 4159.811 kb on + strand at 4159.822 kb on + strand at 4159.822 kb on + strand at 4159.824 kb on - strand at 4159.905 kb on - strand, within yijD at 4159.932 kb on + strand, within yijD at 4159.932 kb on + strand, within yijD at 4159.953 kb on + strand, within yijD at 4159.954 kb on + strand, within yijD at 4159.963 kb on + strand, within yijD at 4159.966 kb on + strand, within yijD at 4160.019 kb on + strand, within yijD at 4160.019 kb on + strand, within yijD at 4160.019 kb on + strand, within yijD at 4160.019 kb on + strand, within yijD at 4160.021 kb on + strand, within yijD at 4160.021 kb on + strand, within yijD at 4160.021 kb on + strand, within yijD at 4160.022 kb on + strand, within yijD at 4160.027 kb on - strand, within yijD at 4160.027 kb on - strand, within yijD at 4160.032 kb on - strand, within yijD at 4160.035 kb on - strand, within yijD at 4160.035 kb on - strand, within yijD at 4160.110 kb on - strand, within yijD at 4160.113 kb on + strand, within yijD at 4160.136 kb on - strand at 4160.177 kb on - strand at 4160.177 kb on - strand at 4160.190 kb on + strand at 4160.196 kb on - strand at 4160.205 kb on + strand at 4160.207 kb on + strand at 4160.207 kb on + strand at 4160.207 kb on + strand at 4160.222 kb on + strand at 4160.222 kb on + strand at 4160.229 kb on - strand at 4160.229 kb on - strand at 4160.236 kb on + strand at 4160.236 kb on + strand at 4160.241 kb on - strand at 4160.244 kb on - strand at 4160.244 kb on - strand at 4160.244 kb on - strand at 4160.244 kb on - strand at 4160.244 kb on - strand at 4160.279 kb on - strand at 4160.291 kb on - strand at 4160.313 kb on + strand, within trmA at 4160.313 kb on + strand, within trmA at 4160.352 kb on - strand, within trmA at 4160.366 kb on + strand, within trmA at 4160.371 kb on + strand, within trmA at 4160.384 kb on - strand, within trmA at 4160.391 kb on - strand, within trmA at 4160.470 kb on + strand, within trmA at 4160.530 kb on - strand, within trmA at 4160.544 kb on + strand, within trmA at 4160.547 kb on - strand, within trmA at 4160.547 kb on - strand, within trmA at 4160.549 kb on - strand, within trmA at 4160.555 kb on - strand, within trmA at 4160.568 kb on - strand, within trmA at 4160.648 kb on - strand, within trmA at 4160.673 kb on + strand, within trmA at 4160.673 kb on + strand, within trmA at 4160.686 kb on + strand, within trmA at 4160.687 kb on - strand, within trmA at 4160.687 kb on - strand, within trmA at 4160.734 kb on - strand, within trmA at 4160.751 kb on - strand, within trmA at 4160.767 kb on - strand, within trmA at 4160.767 kb on - strand, within trmA at 4160.781 kb on + strand, within trmA at 4160.781 kb on - strand, within trmA at 4160.799 kb on - strand, within trmA at 4160.877 kb on - strand, within trmA at 4160.882 kb on - strand, within trmA at 4160.914 kb on + strand, within trmA at 4160.921 kb on + strand, within trmA at 4160.921 kb on - strand, within trmA at 4160.922 kb on + strand, within trmA at 4160.930 kb on - strand, within trmA at 4160.930 kb on - strand, within trmA at 4160.952 kb on - strand, within trmA at 4160.954 kb on + strand, within trmA at 4160.954 kb on + strand, within trmA at 4160.962 kb on - strand, within trmA at 4160.969 kb on - strand, within trmA at 4160.969 kb on - strand, within trmA at 4160.983 kb on - strand, within trmA at 4160.993 kb on + strand, within trmA at 4161.011 kb on - strand, within trmA at 4161.011 kb on - strand, within trmA at 4161.013 kb on + strand, within trmA at 4161.013 kb on + strand, within trmA at 4161.021 kb on - strand, within trmA at 4161.021 kb on - strand, within trmA at 4161.055 kb on - strand, within trmA at 4161.103 kb on + strand, within trmA at 4161.139 kb on + strand, within trmA at 4161.149 kb on - strand, within trmA at 4161.161 kb on + strand, within trmA at 4161.164 kb on - strand, within trmA at 4161.215 kb on + strand at 4161.223 kb on - strand at 4161.259 kb on + strand at 4161.264 kb on - strand at 4161.327 kb on + strand at 4161.337 kb on + strand at 4161.390 kb on - strand at 4161.436 kb on - strand at 4161.446 kb on + strand at 4161.448 kb on - strand at 4161.448 kb on - strand at 4161.537 kb on - strand at 4161.537 kb on - strand at 4161.538 kb on - strand at 4161.548 kb on - strand at 4161.548 kb on - strand at 4161.564 kb on + strand at 4161.564 kb on + strand at 4161.572 kb on - strand at 4161.609 kb on + strand at 4161.618 kb on - strand at 4161.618 kb on - strand at 4161.622 kb on - strand at 4161.622 kb on - strand at 4161.722 kb on - strand at 4161.741 kb on + strand at 4161.741 kb on + strand at 4161.783 kb on - strand at 4161.783 kb on - strand at 4161.791 kb on + strand at 4161.794 kb on - strand at 4161.826 kb on - strand at 4161.826 kb on - strand at 4161.836 kb on - strand at 4161.907 kb on + strand, within btuB at 4161.949 kb on + strand, within btuB at 4161.949 kb on + strand, within btuB at 4161.994 kb on - strand, within btuB at 4161.994 kb on - strand, within btuB at 4161.996 kb on - strand, within btuB at 4162.005 kb on + strand, within btuB at 4162.013 kb on - strand, within btuB at 4162.017 kb on + strand, within btuB at 4162.017 kb on + strand, within btuB at 4162.023 kb on - strand, within btuB at 4162.028 kb on - strand, within btuB at 4162.050 kb on + strand, within btuB at 4162.053 kb on - strand, within btuB at 4162.070 kb on + strand, within btuB at 4162.078 kb on - strand, within btuB at 4162.107 kb on - strand, within btuB at 4162.145 kb on + strand, within btuB at 4162.148 kb on + strand, within btuB at 4162.174 kb on + strand, within btuB at 4162.182 kb on + strand, within btuB at 4162.182 kb on + strand, within btuB at 4162.190 kb on + strand, within btuB at 4162.190 kb on - strand, within btuB at 4162.190 kb on - strand, within btuB at 4162.202 kb on + strand, within btuB at 4162.237 kb on + strand, within btuB at 4162.265 kb on + strand, within btuB at 4162.270 kb on + strand, within btuB at 4162.278 kb on - strand, within btuB
Per-strain Table
Position Strand Gene LocusTag Fraction Trimethoprim 0.0025 mM remove 4,159,215 - fabR b3963 0.10 -1.0 4,159,220 - fabR b3963 0.11 -1.1 4,159,220 - fabR b3963 0.11 -1.6 4,159,237 - fabR b3963 0.14 -0.6 4,159,237 - fabR b3963 0.14 -0.3 4,159,238 + fabR b3963 0.14 -1.7 4,159,238 + fabR b3963 0.14 -0.3 4,159,278 - fabR b3963 0.20 -1.1 4,159,291 - fabR b3963 0.22 -1.2 4,159,291 - fabR b3963 0.22 -2.2 4,159,321 + fabR b3963 0.27 -0.2 4,159,351 + fabR b3963 0.31 -1.4 4,159,352 + fabR b3963 0.32 -0.7 4,159,354 - fabR b3963 0.32 -1.4 4,159,356 + fabR b3963 0.32 -0.8 4,159,402 + fabR b3963 0.39 -0.3 4,159,402 + fabR b3963 0.39 -0.1 4,159,403 + fabR b3963 0.40 -0.0 4,159,424 + fabR b3963 0.43 +0.3 4,159,445 + fabR b3963 0.46 -0.8 4,159,559 + fabR b3963 0.64 -0.1 4,159,559 + fabR b3963 0.64 -0.9 4,159,629 + fabR b3963 0.74 +0.1 4,159,643 - fabR b3963 0.77 -1.5 4,159,656 - fabR b3963 0.79 -1.1 4,159,697 + fabR b3963 0.85 +0.0 4,159,697 + fabR b3963 0.85 +0.1 4,159,699 - fabR b3963 0.85 -1.6 4,159,699 - fabR b3963 0.85 -1.8 4,159,726 + fabR b3963 0.89 -1.2 4,159,726 + fabR b3963 0.89 +0.1 4,159,734 - -1.8 4,159,789 + -1.4 4,159,799 - -1.3 4,159,811 + -0.5 4,159,811 + -1.8 4,159,822 + +0.6 4,159,822 + -1.3 4,159,824 - -0.6 4,159,905 - yijD b3964 0.31 -2.0 4,159,932 + yijD b3964 0.38 -1.3 4,159,932 + yijD b3964 0.38 -1.3 4,159,953 + yijD b3964 0.44 -0.4 4,159,954 + yijD b3964 0.44 -1.6 4,159,963 + yijD b3964 0.47 -1.2 4,159,966 + yijD b3964 0.48 -1.6 4,160,019 + yijD b3964 0.62 -0.2 4,160,019 + yijD b3964 0.62 -2.0 4,160,019 + yijD b3964 0.62 -0.8 4,160,019 + yijD b3964 0.62 -1.6 4,160,021 + yijD b3964 0.63 -1.0 4,160,021 + yijD b3964 0.63 -1.1 4,160,021 + yijD b3964 0.63 -1.9 4,160,022 + yijD b3964 0.63 -1.8 4,160,027 - yijD b3964 0.65 -1.0 4,160,027 - yijD b3964 0.65 -2.8 4,160,032 - yijD b3964 0.66 +0.6 4,160,035 - yijD b3964 0.67 -0.6 4,160,035 - yijD b3964 0.67 -1.1 4,160,110 - yijD b3964 0.88 -1.4 4,160,113 + yijD b3964 0.89 -2.1 4,160,136 - -1.0 4,160,177 - -1.6 4,160,177 - -2.1 4,160,190 + -1.4 4,160,196 - +0.3 4,160,205 + -0.4 4,160,207 + -0.3 4,160,207 + -0.4 4,160,207 + +0.7 4,160,222 + +0.9 4,160,222 + -1.0 4,160,229 - -1.3 4,160,229 - +0.3 4,160,236 + +0.9 4,160,236 + +0.1 4,160,241 - +0.5 4,160,244 - -0.6 4,160,244 - -0.3 4,160,244 - +0.6 4,160,244 - -0.0 4,160,244 - -0.1 4,160,279 - +0.3 4,160,291 - +1.3 4,160,313 + trmA b3965 0.11 -0.7 4,160,313 + trmA b3965 0.11 -0.1 4,160,352 - trmA b3965 0.14 +0.2 4,160,366 + trmA b3965 0.16 +0.1 4,160,371 + trmA b3965 0.16 -0.0 4,160,384 - trmA b3965 0.17 +0.3 4,160,391 - trmA b3965 0.18 +0.4 4,160,470 + trmA b3965 0.25 -0.8 4,160,530 - trmA b3965 0.31 +0.2 4,160,544 + trmA b3965 0.32 +0.9 4,160,547 - trmA b3965 0.32 +0.6 4,160,547 - trmA b3965 0.32 -0.4 4,160,549 - trmA b3965 0.32 +0.7 4,160,555 - trmA b3965 0.33 +0.1 4,160,568 - trmA b3965 0.34 -0.2 4,160,648 - trmA b3965 0.41 +0.2 4,160,673 + trmA b3965 0.44 +0.4 4,160,673 + trmA b3965 0.44 +0.2 4,160,686 + trmA b3965 0.45 +0.3 4,160,687 - trmA b3965 0.45 +1.0 4,160,687 - trmA b3965 0.45 -0.3 4,160,734 - trmA b3965 0.49 -0.3 4,160,751 - trmA b3965 0.51 -1.1 4,160,767 - trmA b3965 0.52 -0.1 4,160,767 - trmA b3965 0.52 +0.7 4,160,781 + trmA b3965 0.53 -1.2 4,160,781 - trmA b3965 0.53 +0.1 4,160,799 - trmA b3965 0.55 +1.1 4,160,877 - trmA b3965 0.62 -0.3 4,160,882 - trmA b3965 0.63 -0.9 4,160,914 + trmA b3965 0.65 +0.7 4,160,921 + trmA b3965 0.66 +0.3 4,160,921 - trmA b3965 0.66 -0.4 4,160,922 + trmA b3965 0.66 +0.6 4,160,930 - trmA b3965 0.67 -0.6 4,160,930 - trmA b3965 0.67 -0.6 4,160,952 - trmA b3965 0.69 +0.6 4,160,954 + trmA b3965 0.69 +0.5 4,160,954 + trmA b3965 0.69 +0.1 4,160,962 - trmA b3965 0.70 +0.2 4,160,969 - trmA b3965 0.70 +0.3 4,160,969 - trmA b3965 0.70 +1.1 4,160,983 - trmA b3965 0.72 +1.0 4,160,993 + trmA b3965 0.73 +0.4 4,161,011 - trmA b3965 0.74 +0.2 4,161,011 - trmA b3965 0.74 +0.0 4,161,013 + trmA b3965 0.74 +0.6 4,161,013 + trmA b3965 0.74 +0.4 4,161,021 - trmA b3965 0.75 -0.2 4,161,021 - trmA b3965 0.75 +0.1 4,161,055 - trmA b3965 0.78 -0.6 4,161,103 + trmA b3965 0.83 -0.3 4,161,139 + trmA b3965 0.86 +1.3 4,161,149 - trmA b3965 0.87 +0.7 4,161,161 + trmA b3965 0.88 -0.4 4,161,164 - trmA b3965 0.88 +0.6 4,161,215 + +0.4 4,161,223 - +1.1 4,161,259 + +0.1 4,161,264 - -0.4 4,161,327 + +0.4 4,161,337 + -0.2 4,161,390 - -1.0 4,161,436 - -1.4 4,161,446 + -0.6 4,161,448 - +0.7 4,161,448 - -0.5 4,161,537 - -0.5 4,161,537 - -0.3 4,161,538 - -0.3 4,161,548 - -0.9 4,161,548 - -1.6 4,161,564 + -0.5 4,161,564 + -0.1 4,161,572 - -0.7 4,161,609 + +0.3 4,161,618 - +0.5 4,161,618 - -0.5 4,161,622 - -0.3 4,161,622 - +0.3 4,161,722 - -1.9 4,161,741 + -1.2 4,161,741 + -2.2 4,161,783 - -1.0 4,161,783 - -0.5 4,161,791 + -0.9 4,161,794 - -0.2 4,161,826 - +0.1 4,161,826 - -0.6 4,161,836 - -1.3 4,161,907 + btuB b3966 0.13 +0.1 4,161,949 + btuB b3966 0.16 -1.1 4,161,949 + btuB b3966 0.16 -0.3 4,161,994 - btuB b3966 0.18 -0.3 4,161,994 - btuB b3966 0.18 -0.2 4,161,996 - btuB b3966 0.18 -0.4 4,162,005 + btuB b3966 0.19 -1.2 4,162,013 - btuB b3966 0.19 -1.4 4,162,017 + btuB b3966 0.19 +0.5 4,162,017 + btuB b3966 0.19 -1.1 4,162,023 - btuB b3966 0.20 -0.7 4,162,028 - btuB b3966 0.20 -0.0 4,162,050 + btuB b3966 0.21 -1.0 4,162,053 - btuB b3966 0.21 -0.4 4,162,070 + btuB b3966 0.22 -0.4 4,162,078 - btuB b3966 0.23 -0.9 4,162,107 - btuB b3966 0.24 -0.3 4,162,145 + btuB b3966 0.26 +0.0 4,162,148 + btuB b3966 0.26 -1.1 4,162,174 + btuB b3966 0.28 -0.7 4,162,182 + btuB b3966 0.28 -0.6 4,162,182 + btuB b3966 0.28 -1.4 4,162,190 + btuB b3966 0.29 -2.0 4,162,190 - btuB b3966 0.29 -0.2 4,162,190 - btuB b3966 0.29 -0.3 4,162,202 + btuB b3966 0.29 -0.7 4,162,237 + btuB b3966 0.31 -0.4 4,162,265 + btuB b3966 0.33 -0.7 4,162,270 + btuB b3966 0.33 -0.6 4,162,278 - btuB b3966 0.33 -0.6
Or see this region's nucleotide sequence