Strain Fitness in Escherichia coli BW25113 around b3177

Experiment: Trimethoprim 0.0025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntglmM and folP overlap by 8 nucleotidesfolP and ftsH are separated by 89 nucleotides b3176: glmM - phosphoglucosamine mutase (NCBI), at 3,320,755 to 3,322,092 glmM b3177: folP - 7,8-dihydropteroate synthase (VIMSS), at 3,322,085 to 3,322,933 folP b3178: ftsH - protease, ATP-dependent zinc-metallo (NCBI), at 3,323,023 to 3,324,957 ftsH Position (kb) 3322 3323Strain fitness (log2 ratio) -1 0 1at 3322.094 kb on - strandat 3322.095 kb on - strandat 3322.096 kb on - strandat 3322.096 kb on - strandat 3322.115 kb on - strandat 3322.944 kb on - strandat 3322.944 kb on - strandat 3323.027 kb on - strandat 3323.048 kb on - strandat 3323.048 kb on - strandat 3323.059 kb on - strandat 3323.059 kb on - strandat 3323.059 kb on - strandat 3323.074 kb on - strandat 3323.092 kb on - strandat 3323.096 kb on - strandat 3323.105 kb on - strandat 3323.105 kb on - strandat 3323.168 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Trimethoprim 0.0025 mM
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3,322,094 - -0.0
3,322,095 - -0.1
3,322,096 - -0.8
3,322,096 - -1.6
3,322,115 - +0.0
3,322,944 - -0.7
3,322,944 - -0.6
3,323,027 - +0.6
3,323,048 - -0.7
3,323,048 - +0.4
3,323,059 - +1.1
3,323,059 - -0.7
3,323,059 - -0.5
3,323,074 - +0.6
3,323,092 - -0.1
3,323,096 - -0.3
3,323,105 - +0.3
3,323,105 - +0.9
3,323,168 - -0.1

Or see this region's nucleotide sequence