Strain Fitness in Escherichia coli BW25113 around b2531

Experiment: Trimethoprim 0.0025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntiscS and iscR are separated by 111 nucleotidesiscR and yfhQ are separated by 451 nucleotides b2530: iscS - cysteine desulfurase (NCBI), at 2,658,339 to 2,659,553 iscS b2531: iscR - DNA-binding transcriptional repressor (NCBI), at 2,659,665 to 2,660,153 iscR b2532: yfhQ - predicted methyltransferase (NCBI), at 2,660,605 to 2,661,345 yfhQ Position (kb) 2659 2660 2661Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2659.636 kb on - strandat 2659.636 kb on - strandat 2659.684 kb on - strandat 2659.834 kb on - strand, within iscRat 2659.875 kb on - strand, within iscRat 2659.897 kb on - strand, within iscRat 2660.133 kb on - strandat 2660.376 kb on + strandat 2660.376 kb on + strandat 2660.376 kb on + strandat 2660.376 kb on + strandat 2660.475 kb on - strandat 2660.558 kb on + strandat 2661.004 kb on + strand, within yfhQat 2661.004 kb on + strand, within yfhQat 2661.012 kb on - strand, within yfhQat 2661.023 kb on - strand, within yfhQat 2661.088 kb on - strand, within yfhQ

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Per-strain Table

Position Strand Gene LocusTag Fraction Trimethoprim 0.0025 mM
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2,659,636 - +1.8
2,659,636 - +1.0
2,659,684 - +0.1
2,659,834 - iscR b2531 0.35 +2.0
2,659,875 - iscR b2531 0.43 +3.7
2,659,897 - iscR b2531 0.47 +1.5
2,660,133 - +3.4
2,660,376 + -0.1
2,660,376 + -0.7
2,660,376 + +0.1
2,660,376 + +0.5
2,660,475 - -1.1
2,660,558 + -0.6
2,661,004 + yfhQ b2532 0.54 -1.2
2,661,004 + yfhQ b2532 0.54 -1.0
2,661,012 - yfhQ b2532 0.55 -1.6
2,661,023 - yfhQ b2532 0.56 -2.0
2,661,088 - yfhQ b2532 0.65 -0.8

Or see this region's nucleotide sequence