Strain Fitness in Escherichia coli BW25113 around b2319

Experiment: Trimethoprim 0.0025 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Trimethoprim 0.0025 mM
2,432,800	+				-0.7
2,432,808	-				-0.6
2,432,808	-				-0.5
2,433,004	+	truA	b2318	0.19	+1.7
2,433,141	+	truA	b2318	0.36	+2.1
2,433,141	+	truA	b2318	0.36	+1.5
2,433,157	+	truA	b2318	0.38	+1.5
2,433,160	-	truA	b2318	0.39	+1.6
2,433,160	-	truA	b2318	0.39	-0.0
2,433,170	-	truA	b2318	0.40	-0.5
2,433,363	-				+1.8
2,433,465	-	truA	b2318	0.76	+0.7
2,433,465	-	truA	b2318	0.76	+0.6
2,433,465	-	truA	b2318	0.76	+0.5
2,433,465	-	truA	b2318	0.76	-0.9
2,433,600	+				+0.5
2,433,600	+				+2.6
2,433,656	+				-0.9
2,433,664	-				-1.3
2,433,670	+				+4.6
2,433,670	+				+1.6
2,433,678	-				+0.1
2,433,753	-				-0.3
2,433,846	+	usg	b2319	0.19	+2.9
2,433,853	-	usg	b2319	0.19	-0.9
2,433,853	-	usg	b2319	0.19	-1.0
2,433,910	+	usg	b2319	0.25	+2.8
2,433,943	+	usg	b2319	0.28	+2.4
2,433,952	-	usg	b2319	0.29	-0.1
2,433,952	-	usg	b2319	0.29	-0.1
2,434,057	+	usg	b2319	0.39	+0.8
2,434,167	-	usg	b2319	0.50	-1.3
2,434,287	-	usg	b2319	0.62	+0.4
2,434,287	-	usg	b2319	0.62	+0.1
2,434,339	+	usg	b2319	0.67	+2.4
2,434,360	+	usg	b2319	0.69	+1.2
2,434,626	-				-1.1
2,434,647	+				+1.2
2,434,647	+				+2.1
2,434,690	-				-1.3
2,434,711	+				+0.1
2,434,761	+				+0.1
2,434,787	+				-2.4
2,434,881	-	pdxB	b2320	0.13	-0.6
2,434,881	-	pdxB	b2320	0.13	-1.0
2,434,932	+	pdxB	b2320	0.17	+0.9
2,434,932	+	pdxB	b2320	0.17	-0.2
2,434,999	-	pdxB	b2320	0.23	-0.9
2,435,038	-	pdxB	b2320	0.26	-0.0
2,435,105	-				-0.6
2,435,110	+	pdxB	b2320	0.33	+0.4
2,435,110	+	pdxB	b2320	0.33	+1.8
2,435,230	+	pdxB	b2320	0.43	+0.6
2,435,230	+	pdxB	b2320	0.43	+0.6
2,435,246	+	pdxB	b2320	0.45	-0.9
2,435,285	+	pdxB	b2320	0.48	+0.1
2,435,324	+	pdxB	b2320	0.52	-0.5
2,435,324	+	pdxB	b2320	0.52	-0.3
2,435,343	-	pdxB	b2320	0.53	-0.3
2,435,511	+	pdxB	b2320	0.68	+0.3